NanoLuc® (Nluc) Luciferase Antibody

Catalog # Availability Size / Price Qty
Detection  of NanoLuc® Luciferase and Large BiT by Western Blot.
1 Image
Product Details
Supplemental Products

NanoLuc® (Nluc) Luciferase Antibody Summary

Detects NanoLuc® (Nluc) Luciferase in direct ELISAs. Detects NanoLuc® (Nluc) Luciferase and Large BiT in Western blots.
Monoclonal Mouse IgG2B Clone # 965808
Protein A or G purified from hybridoma culture supernatant
Synthetic peptide of NanoLuc® (Nluc) Luciferase
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection  of NanoLuc® Luciferase and Large BiT by Western Blot. View Larger

Detection of NanoLuc® Luciferase and Large BiT by Western Blot. Western blot shows Recombinant Large BiT and Recombinant NanoLuc®. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human NanoLuc® (Nluc) Luciferase Monoclonal Antibody (Catalog # MAB10026) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for NanoLuc® and Large BiT at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Luciferase

NanoLuc® (Nluc) luciferase is a small enzyme (19.1kDa) engineered for optimal performance as a luminescent reporter. NanoLuc® Binary Technology (NanoBiT™) is a two subunit system based on NanoLuc® luciferase. Large BiT (LgBiT; 17.6kDa) is the large subunit of the NanoBiT® system.


Alternate Names
ec; Fluc; Luciferase; luciferin 4 monooxygenase

Product Datasheets


No product specific FAQs exist for this product, however you may

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2B Antibody

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