NanoLuc® (Nluc) Luciferase Antibody
NanoLuc® (Nluc) Luciferase Antibody Summary
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of NanoLuc® Luciferase and Large BiT by Western Blot. Western blot shows Recombinant Large BiT and Recombinant NanoLuc®. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human NanoLuc® (Nluc) Luciferase Monoclonal Antibody (Catalog # MAB10026) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for NanoLuc® and Large BiT at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Luciferase
NanoLuc® (Nluc) luciferase is a small enzyme (19.1kDa) engineered for optimal performance as a luminescent reporter. NanoLuc® Binary Technology (NanoBiT™) is a two subunit system based on NanoLuc® luciferase. Large BiT (LgBiT; 17.6kDa) is the large subunit of the NanoBiT® system.
Product Datasheets
Product Specific Notices
NanoLuc and NanoBiT are registered trademarks of Promega Corporation.Citations for NanoLuc® (Nluc) Luciferase Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Real-time tracking of bioluminescent influenza A virus infection in mice
Authors: JH Kim, H Bryant, E Fiedler, T Cao, JO Rayner
Scientific Reports, 2022;12(1):3152.
Species: Canine
Sample Types: Cell Lysates
Applications: Western Blot -
PAX8 and MECOM are interaction partners driving ovarian cancer
Authors: M Bleu, F Mermet-Mei, V Apfel, L Barys, L Holzer, M Bachmann S, R Lopes, I Amorim Mon, C Delmas, A Hinniger, S Chau, M Kaufmann, S Haenni, K Berneiser, M Wahle, I Moravec, A Vissières, T Poetsch, E Ahrné, N Carte, J Voshol, E Bechter, J Hamon, M Meyerhofer, D Erdmann, M Fischer, T Stachyra, F Freuler, S Gutmann, C Fernández, T Schmelzle, U Naumann, G Roma, K Lawrenson, C Nieto-Ober, A Cobos-Corr, S Ferretti, D Schübeler, GG Galli
Nature Communications, 2021;12(1):2442.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
A point mutation decouples the lipid transfer activities of microsomal triglyceride transfer protein
Authors: MH Wilson, S Rajan, A Danoff, RJ White, MR Hensley, VH Quinlivan, R Recacha, JH Thierer, FJ Tan, EM Busch-Nent, L Ruddock, MM Hussain, SA Farber
PLoS Genet., 2020;16(8):e1008941.
Species: Zebrafish
Sample Types: Tissue Homogenates
Applications: Western Blot
FAQs
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What is the immunogen for the NanoLuc® (Nluc) Luciferase Antibody, Catalog # MAB10026? Does this antibody recognize only LgBiT and not SmBiT?
The immunoogen for Catalog # MAB10026 is Synthetic peptide of NanoLuc® (Nluc) Luciferase. For more details on the compound, Promega Corporation should be contacted. NanoBit (LgBit+SmBit) is a derivative of the original product NanoLuc, where portion of the protein is split to get two complementary peptides. This antibody detects LgBiT and not SmBiT.
Isotype Controls
Reconstitution Buffers
Secondary Antibodies
Reviews for NanoLuc® (Nluc) Luciferase Antibody
Average Rating: 4.5 (Based on 2 Reviews)
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