NanoLuc® (Nluc) Luciferase Antibody

Catalog # Availability Size / Price Qty
Detection of NanoLuc® Luciferase and Large BiT by Western Blot.
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Product Details
Citations (3)
Supplemental Products
Reviews (2)

NanoLuc® (Nluc) Luciferase Antibody Summary

Detects NanoLuc® (Nluc) Luciferase in direct ELISAs. Detects NanoLuc® (Nluc) Luciferase and Large BiT in Western blots.
Monoclonal Mouse IgG2B Clone # 965808
Protein A or G purified from hybridoma culture supernatant
Synthetic peptide of NanoLuc® (Nluc) Luciferase
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Recommended Concentration
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of NanoLuc® Luciferase and Large BiT antibody by Western Blot. View Larger

Detection of NanoLuc® Luciferase and Large BiT by Western Blot. Western blot shows Recombinant Large BiT and Recombinant NanoLuc®. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human NanoLuc® (Nluc) Luciferase Monoclonal Antibody (Catalog # MAB10026) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for NanoLuc® and Large BiT at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Luciferase

NanoLuc® (Nluc) luciferase is a small enzyme (19.1kDa) engineered for optimal performance as a luminescent reporter. NanoLuc® Binary Technology (NanoBiT™) is a two subunit system based on NanoLuc® luciferase. Large BiT (LgBiT; 17.6kDa) is the large subunit of the NanoBiT® system.


Alternate Names
ec; firefly luciferase; Fluc; LuC; Luciferase; luciferin 4 monooxygenase; Luciferin 4-monooxygenase

Product Datasheets

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Product Specific Notices

NanoLuc and NanoBiT are registered trademarks of Promega Corporation.

Citations for NanoLuc® (Nluc) Luciferase Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Real-time tracking of bioluminescent influenza A virus infection in mice
    Authors: JH Kim, H Bryant, E Fiedler, T Cao, JO Rayner
    Scientific Reports, 2022;12(1):3152.
    Species: Canine
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. PAX8 and MECOM are interaction partners driving ovarian cancer
    Authors: M Bleu, F Mermet-Mei, V Apfel, L Barys, L Holzer, M Bachmann S, R Lopes, I Amorim Mon, C Delmas, A Hinniger, S Chau, M Kaufmann, S Haenni, K Berneiser, M Wahle, I Moravec, A Vissières, T Poetsch, E Ahrné, N Carte, J Voshol, E Bechter, J Hamon, M Meyerhofer, D Erdmann, M Fischer, T Stachyra, F Freuler, S Gutmann, C Fernández, T Schmelzle, U Naumann, G Roma, K Lawrenson, C Nieto-Ober, A Cobos-Corr, S Ferretti, D Schübeler, GG Galli
    Nature Communications, 2021;12(1):2442.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. A point mutation decouples the lipid transfer activities of microsomal triglyceride transfer protein
    Authors: MH Wilson, S Rajan, A Danoff, RJ White, MR Hensley, VH Quinlivan, R Recacha, JH Thierer, FJ Tan, EM Busch-Nent, L Ruddock, MM Hussain, SA Farber
    PLoS Genet., 2020;16(8):e1008941.
    Species: Zebrafish
    Sample Types: Tissue Homogenates
    Applications: Western Blot


  1. What is the immunogen for the NanoLuc® (Nluc) Luciferase Antibody, Catalog # MAB10026? Does this antibody recognize only LgBiT and not SmBiT?

    • The immunoogen for Catalog # MAB10026 is Synthetic peptide of NanoLuc® (Nluc) Luciferase. For more details on the compound, Promega Corporation should be contacted. NanoBit (LgBit+SmBit) is a derivative of the original product NanoLuc,  where portion of the protein is split to get two complementary peptides. This antibody detects LgBiT and not SmBiT.

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for NanoLuc® (Nluc) Luciferase Antibody

Average Rating: 4.5 (Based on 2 Reviews)

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NanoLuc® (Nluc) Luciferase Antibody
By Franziska Gsottberger on 04/28/2020
Application: ICC/IF Sample Tested: HEK293T human embryonic kidney cell line Species: Human

NanoLuc® (Nluc) Luciferase Antibody
By Juan Guzman on 04/23/2020
Application: ICC/IF Sample Tested: HEK293 human embryonic kidney cell line Species: Human