|Cell Proliferation Induced by IL‑1 alpha /IL‑1F1 and Neutralization by Porcine IL‑1 alpha /IL‑1F1 Antibody. Recombinant Porcine IL‑1 alpha /IL‑1F1 (Catalog # 680-PI) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL‑1 alpha /IL‑1F1 (75 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IL‑1 alpha /IL‑1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF680). The ND50 is typically 0.006-0.012 µg/mL in the presence of concanavalin A (1.25 µg/mL).|
Interleukin 1 (IL-1) is a name that designates two proteins, IL-1 alpha and IL-1 beta, that are the products of distinct genes, but show approximately 25% amino acid sequence identity and recognize the same cell surface receptors. Although IL-1 production is generally considered to be a consequence of inflammation, evidence suggests that IL-1 is also temporarily upregulated during bone formation and the menstrual cycle and can be induced in response to nervous system stimulation. In response to stimuli produced by inflammatory agents, infections, or microbial endotoxins, a dramatic increase in the production of IL-1 by macrophages and various other cells is seen. Cells in particular known to produce IL-1 include osteoblasts, monocytes, macrophages, keratinocytes, Kupffer cells, hepatocytes, thymic and salivary gland epithelium, Schwann cells, fibroblasts and glia (oligodendroglia, astrocytes and microglia).
IL-1 alpha and IL-1 beta are both synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17,000. Neither precursor contains a typical hydrophobic signal peptide sequence and most of the precursor form of IL-1 alpha remains in the cytosol of cells, although there is evidence for a membrane-bound form of the precursor form of IL-1 alpha. The IL-1 alpha precursor reportedly shows full biological activity in the EL-4 assay. Among various species, the amino acid sequence of mature IL-1 alpha is conserved 60% to 70% and porcine IL-1 has been found to be biologically active on murine cell lines. Both forms of IL-1 bind to the same receptors, designated as type I and type II. Evidence suggests that only the type I receptor is capable of signal transduction and that the type II receptor may function as a decoy, binding IL-1 and thus preventing the binding of IL-1 to the type I receptor.
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