Porcine IL-1 alpha /IL-1F1 Antibody

Catalog # Availability Size / Price Qty
AF680
AF680-SP
Porcine IL-1 alpha /IL-1F1 Antibody in Data
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Citations (2)
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Porcine IL-1 alpha /IL-1F1 Antibody Summary

Species Reactivity
Porcine
Specificity
Detects porcine IL‑1 alpha /IL‑1F1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human IL-1 alpha  is observed and 10% cross-reactivity with recombinant mouse IL-1 alpha and recombinant rat IL-1 alpha is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant porcine IL-1 alpha
Gln119-Ser270
Accession # P18430
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Porcine IL‑1 alpha /IL‑1F1 (Catalog # 680-PI)
Neutralization
Measured by its ability to neutralize IL‑1 alpha /IL‑1F1-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J. A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND50) is typically 0.006-0.012 µg/mL in the presence of 75 pg/mL Recombinant Porcine IL‑1 alpha /IL‑1F1 and 1.25 µg/mL concanavalin A.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization Cell Proliferation Induced by IL‑1 alpha /IL‑1F1 and Neutral-ization by Porcine IL‑1 alpha /IL‑1F1 Antibody. View Larger

Cell Proliferation Induced by IL‑1 alpha /IL‑1F1 and Neutral-ization by Porcine IL‑1 alpha /IL‑1F1 Antibody. Recombinant Porcine IL-1a/IL-1F1 (Catalog # 680-PI) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL-1a/IL-1F1 (75 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IL-1a/IL-1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF680). The ND50is typically 0.006-0.012 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 alpha/IL-1F1

Interleukin 1 (IL-1) is a name that designates two proteins, IL-1 alpha  and IL-1 beta, that are the products of distinct genes, but show approximately 25% amino acid sequence identity and recognize the same cell surface receptors. Although IL-1 production is generally considered to be a consequence of inflammation, evidence suggests that IL-1 is also temporarily upregulated during bone formation and the menstrual cycle and can be induced in response to nervous system stimulation. In response to stimuli produced by inflammatory agents, infections, or microbial endotoxins, a dramatic increase in the production of IL-1 by macrophages and various other cells is seen. Cells in particular known to produce IL-1 include osteoblasts, monocytes, macrophages, keratinocytes, Kupffer cells, hepatocytes, thymic and salivary gland epithelium, Schwann cells, fibroblasts and glia (oligodendroglia, astrocytes and microglia).

IL-1 alpha and IL-1 beta are both synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17,000. Neither precursor contains a typical hydrophobic signal peptide sequence and most of the precursor form of IL-1 alpha remains in the cytosol of cells, although there is evidence for a membrane-bound form of the precursor form of IL-1 alpha. The IL-1 alpha precursor reportedly shows full biological activity in the EL-4 assay. Among various species, the amino acid sequence of mature IL-1 alpha is conserved 60% to 70% and porcine IL-1 has been found to be biologically active on murine cell lines. Both forms of IL-1 bind to the same receptors, designated as type I and type II. Evidence suggests that only the type I receptor is capable of signal transduction and that the type II receptor may function as a decoy, binding IL-1 and thus preventing the binding of IL-1 to the type I receptor.

References
  1. Dower, S.K. and J.E. Sims, in Guidebook to Cytokines and their receptors (1994) N.A. Nicole ed. Oxford University Press NY p. 17.
Long Name
Interleukin 1 alpha
Entrez Gene IDs
3552 (Human); 16175 (Mouse); 24493 (Rat); 397094 (Porcine)
Alternate Names
BAF; Hematopoietin-1; IL1 alpha; IL-1 alpha; IL1; IL1A; IL-1A; IL1-ALPHA; IL1F1; IL-1F1; IL1F1hematopoietin-1; interleukin 1, alpha; interleukin-1 alpha; LAF; LEM; preinterleukin 1 alpha; pro-interleukin-1-alpha

Product Datasheets

Citations for Porcine IL-1 alpha /IL-1F1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Proteasome inhibition can impair caspase-8 activation upon submaximal stimulation of apoptotic tumor necrosis factor-related apoptosis inducing ligand (TRAIL) signaling.
    Authors: Laussmann MA, Passante E, Hellwig CT
    J. Biol. Chem., 2012;287(18):14402-11.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Induction of interleukin-1 in articular cartilage by explantation and cutting.
    Authors: Gruber J, Vincent TL, Hermansson M, Bolton M, Wait R, Saklatvala J
    Arthritis Rheum., 2004;50(8):2539-46.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Neutralization

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