Porcine IL-8/CXCL8 Antibody

Catalog # Availability Size / Price Qty
Chemotaxis Induced by IL-8/CXCL8 and Neutralization by Porcine CXCL8/IL‑8 Antibody.
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Product Details
Citations (2)
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Reviews (2)

Porcine IL-8/CXCL8 Antibody Summary

Species Reactivity
Detects human IL-8/CXCL8 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human IL-8 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant porcine IL-8/CXCL8
Accession # CAA43461
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Porcine IL-8/CXCL8 (Catalog # 535-IN)
Measured by its ability to neutralize IL-8/CXCL8-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 1.5-7.5 µg/mL in the presence of 50 ng/mL Recombinant Porcine IL-8/CXCL8.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by IL-8/CXCL8 and Neutralization by Porcine CXCL8/IL‑8 Antibody. View Larger

Chemotaxis Induced by IL-8/CXCL8 and Neutralization by Porcine CXCL8/IL‑8 Antibody. Recombinant Porcine IL-8/CXCL8 (Catalog # 535-IN) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Porcine IL-8/CXCL8 (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF535). The ND50 is typically 1.5-7.5 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-8/CXCL8

Interleukin 8 was originally discovered and purified independently by a number of laboratories as a neutrophil chemotactic and activating factor. It was also referred to as neutrophil chemotactic factor (NCF), neutrophil activating protein (NAP), monocyte-derived neutrophil chemotactic factor (MDNCF), T-lymphocyte chemotactic factor (TCF), granulocyte chemotactic protein (GCP) and leukocyte adhesion inhibitor (LAI). Many cell types, including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, chondrocytes, and various tumor cell lines, can produce IL-8 in response to a wide variety of pro-inflammatory stimuli such as exposure to IL-1, TNF, LPS, and viruses. IL-8 is a member of the alpha (C-X-C) subfamily of chemokines, which also includes platelet factor 4, GRO, IP-10, etc.

IL-8 is a potent chemoattractant for neutrophils. In addition, IL-8 also has a wide range of other pro-inflammatory effects. IL-8 causes degranulation of neutrophil specific granules and azurophilic granules. IL-8 induces expression of the cell adhesion molecules CD11/CD18 and enhances the adherence of neutrophils to endothelial cells and sub-endothelial matrix proteins. Besides neutrophils, IL-8 is also chemotactic for basophils, T cells and eosinophils. IL-8 has been reported to be a co-mitogen for keratinocytes and was also shown to be an autocrine growth factor for melanoma cells. Recently, IL-8 was reported to be angiogenic both in vivo and in vitro.

  1. Van Damme, J. et al. (1998) in The Cytokine Handbook, A.W. Thomson ed., Academic Press, New York. p. 271.
Long Name
Interleukin 8
Entrez Gene IDs
3576 (Human); 396880 (Porcine); 403850 (Canine); 493836 (Feline)
Alternate Names
3-10C; AMCF-I; C-X-C motif chemokine 8; CXCL8; CXCL8SCYB8; Emoctakin; GCP1; GCP-1TSG-1; IL8; IL-8; interleukin 8; K60; LAI; LECT; LUCT; LYNAP; MDNCF; MDNCFb-ENAP; member 8; MONAP; MONAPGCP1; NAF; NAP1; NAP-1NAP1; NCF; Neutrophil-activating protein 1; Protein 3-10C; T cell chemotactic factor; T-cell chemotactic factor; TCF; TSG1

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Citations for Porcine IL-8/CXCL8 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Matrine inhibits IL-1? secretion in primary porcine alveolar macrophages through the MyD88/NF-κB pathway and NLRP3 inflammasome
    Authors: P Sun, N Sun, W Yin, Y Sun, K Fan, J Guo, A Khan, Y He, H Li
    Vet. Res., 2019;50(1):53.
    Species: Porcine
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  2. Exposure to hog barn dust alters airway epithelial ciliary beating.
    Authors: Wyatt TA, Sisson JH, Von Essen SG, Poole JA, Romberger DJ
    Eur. Respir. J., 2008;31(6):1249-55.
    Species: Porcine
    Sample Types: Complex Sample Type
    Applications: Western Blot


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Isotype Controls

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Secondary Antibodies

Goat IgG (H+L) Antibody


Reviews for Porcine IL-8/CXCL8 Antibody

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Porcine IL-8/CXCL8 Antibody
By Zoia Levashova on 10/18/2018
Application: MSD assay Sample Tested: Vitreous humor Species: Porcine

After labeling with Sulfo-Tag, used as a detection reagent in MSD assay (Meso Scale Diagnostics LLC). Paired with biotinylated IL-8 antibody (MAB535) as a capture reagent. A standard curve with recombinant porcine IL-8 from R&D (Cat# 535-IN/CF) is shown (2-25,000 pg/ml).

Porcine IL-8/CXCL8 Antibody
By Anonymous on 11/08/2017
Application: ELISA Sample Tested: Porcine Serum,Serum and Plasma Species: Porcine

The antibody MAB5351 was used as the capture antibody in a sandwich ELISA for Porcine IL-8 along with the detection antibody, AF535. The calibrator for the assay was recombinant porcine IL-8, 535-IN. It was sensitive and specific.