Proteome Profiler Human Cell Stress Array Kit

Contains 4 membranes - each spotted in duplicate with 26 different cell stress antibodies
Catalog # Availability Size / Price Qty
Determination of Relative Levels of Selected Human Cell Stress-related Proteins.
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Product Details
Citations (17)
Reviews (3)

Proteome Profiler Human Cell Stress Array Kit Summary

Kit Summary

A membrane-based antibody array for the parallel determination of the relative levels of selected human cell stress-related proteins. Validated for analyte detection in cell and tissue lysates.

Troubleshooting Guide

Key Benefits

  • Detects 26 human cell stress-related proteins simultaneously
  • Requires no specialized equipment

Principle of the Array

The Proteome Profiler Human Cell Stress Array Kit is a membrane-based sandwich immunoassay. Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2). Captured proteins are visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.

Why Use an Antibody Array to Detect Cell Stress-Related Proteins?

Determining the expression of multiple cell stress-related proteins in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple proteins in a single sample can be cost-effective and also save time and sample.


Kit Contents
  • 4 Array Membranes
  • 4-Well Multi-dish
  • Array Buffers
  • Lysis Buffer
  • Wash Buffer
  • Detection Antibody Cocktail
  • Streptavidin-HRP
  • Chemiluminescent Detection Reagents
  • Transparency Overlay Template
  • Detailed Protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

Stability and Storage

Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.


Simultaneously detect the levels of these cell stress-related proteins in a single sample.
ADAMTS1 HIF-2 alpha Phospho-p38 alpha (T180/Y182)
Bcl-2 Phospho-HSP27 (S78/S82) Phospho-p53 (S46)
Carbonic Anhydrase IX HSP60 PON1
Cited-2 HSP70 PON2
Cytochrome c Phospho-JNK Pan (T183/Y185) Thioredoxin-1
Dkk-4 NFkappaB1 SIRT2
HIF-1 alpha p27/Kip1  


Assays for analytes represented in the Human Cell Stress Array Kit

Analyte Quantkine® ELISA Kits DuoSet® ELISA Development Systems DuoSet® IC ELISA Development Systems (Total) DuoSet® IC ELISA Development Systems (Phospho)
ADAMTS1   DY2197    
Bcl-2     DYC827B  
Carbonic Anhydrase IX DCA900 DY2188    
COX-2     DYC4198  
Cytochrome c DCTC0      
Dkk-4   DY1269    
HIF-1 alpha     DYC1935  
HIF-2 alpha        
Phospho-HSP27 (S78/S82)     DYC1580 DYC2314
HSP60     DYC1800  
HSP70   DY1663 DYC1663  
IDO   DY6030    
Phospho-JNK Pan (T183/Y185)     DYC1387B DYC2236
p21/CIP1     DYC1047  
p27/Kip1     DYC2256  
Phospho-p38 alpha (T180/Y182)     DYC8691B DYC869B
Phospho-p53 (S46)     DYC1043  
PON1     DYC5816  
SOD2     DYC3419  


Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Product Datasheets

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Scientific Data

Determination of Relative Levels of Selected Human Cell Stress-related Proteins. HeLa human cervical epithelial carcinoma cells were either untreated or exposed to 150 J/m2 of UV light followed by a 30 minute recovery period before lysis.

Determination of Relative Levels of Selected Human Cell Stress-related Proteins. The Human Cell Stress Array detects multiple analytes in tissue lysates. 300 µg of tissue lysate was run on each array. Data shown are from a 5 minute exposure to X-ray film.

Citations for Proteome Profiler Human Cell Stress Array Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. The Phytochemical Profiling, In Vitro Antioxidant, and Hepatoprotective Activity of Prenanthes purpurea L. and Caffeoylquinic Acids in Diclofenac-Induced Hepatotoxicity on HEP-G2 Cells
    Authors: Mihaylova, R;Gevrenova, R;Stefanova, A;Zheleva-Dimitrova, D;Balabanova, V;Zengin, G;Simeonova, R;Momekov, G;
    International journal of molecular sciences  2023-09-15
  2. Splicing factor SRSF3 represses translation of p21cip1/waf1 mRNA
    Authors: J Kim, RY Park, Y Kee, S Jeong, T Ohn
    Cell Death & Disease, 2022-11-07;13(11):933.  2022-11-07
  3. Oxidative Stress Response in Adipose Tissue-Derived Mesenchymal Stem/Stromal Cells
    Authors: TO Waheed, O Hahn, K Sridharan, C Mörke, G Kamp, K Peters
    International Journal of Molecular Sciences, 2022-11-03;23(21):.  2022-11-03
  4. The Cardenolide Glycoside Acovenoside A Interferes with Epidermal Growth Factor Receptor Trafficking in Non-Small Cell Lung Cancer Cells
    Authors: S Hafner, M Schmiech, SJ Lang
    Frontiers in Pharmacology, 2021-05-05;12(0):611657.  2021-05-05
  5. Impairment of Hypoxia-Induced CA IX by Beta-Blocker Propranolol-Impact on Progression and Metastatic Potential of Colorectal Cancer Cells
    Authors: M Barathova, K Grossmanno, P Belvonciko, V Kubasova, V Simko, R Skubla, L Csaderova, J Pastorek
    Int J Mol Sci, 2020-11-19;21(22):.  2020-11-19
  6. Decreased Thymic Output Contributes to Immune Defects in Septic Patients
    Authors: N Sommer, S Noack, A Hecker, H Hackstein, G Bein, N Weissmann, W Seeger, K Mayer, M Hecker
    J Clin Med, 2020-08-20;9(9):.  2020-08-20
  7. Sequesterpene Lactones Isolated from a Brazilian Cerrado Plant (Eremanthus spp.) as Anti-Proliferative Compounds, Characterized by Functional and Proteomic Analysis, are Candidates for New Therapeutics in Glioblastoma
    Authors: C Izumi, HJ Laure, NG Barbosa, C Hassibe Th, G Aguiar Fer, J Paulo Barr, N Peporine L, J César Rosa
    Int J Mol Sci, 2020-07-01;21(13):.  2020-07-01
  8. Secondary Metabolites from the Culture of the Marine-derived Fungus Paradendryphiella salina PC 362H and Evaluation of the Anticancer Activity of Its Metabolite Hyalodendrin
    Authors: A Dezaire, CH Marchand, M Vallet, N Ferrand, S Chaouch, E Mouray, AK Larsen, M Sabbah, SD Lemaire, S Prado, AE Escargueil
    Mar Drugs, 2020-04-03;18(4):.  2020-04-03
  9. Semi-Synthetic Ingenol Derivative from Euphorbia tirucalli Inhibits Protein Kinase C Isotypes and Promotes Autophagy and S-phase Arrest on Glioma Cell Lines
    Authors: VAO Silva, MN Rosa, A Tansini, O Martinho, A Tanuri, AF Evangelist, A Cruvinel C, JP Lima, LF Pianowski, RM Reis
    Molecules, 2019-11-22;24(23):.  2019-11-22
  10. Fundamental differences in patterns of retinal ageing between primates and mice
    Authors: JH Kam, TW Weinrich, H Shinhmar, MB Powner, NW Roberts, A Aboelnour, G Jeffery
    Sci Rep, 2019-08-29;9(1):12574.  2019-08-29
  11. Preclinical evaluation of an unconventional ruthenium-gold-based chemotherapeutic: RANCE-1, in clear cell renal cell carcinoma
    Authors: BT Elie, K Hubbard, Y Pechenyy, B Layek, S Prabha, M Contel
    Cancer Med, 2019-06-13;0(0):.  2019-06-13
  12. Remote ischemic preconditioning attenuates intestinal mucosal damage: insight from a rat model of ischemia-reperfusion injury
    Authors: L Hummitzsch, K Zitta, R Berndt, YL Wong, R Rusch, K Hess, T Wedel, M Gruenewald, J Cremer, M Steinfath, M Albrecht
    J Transl Med, 2019-04-29;17(1):136.  2019-04-29
  13. Neuronal AMP-activated protein kinase hyper-activation induces synaptic loss by an autophagy-mediated process
    Authors: M Domise, F Sauvé, S Didier, R Caillerez, S Bégard, S Carrier, M Colin, C Marinangel, L Buée, V Vingtdeux
    Cell Death Dis, 2019-03-04;10(3):221.  2019-03-04
  14. Endoplasmic reticulum stress stimulates the release of extracellular vesicles carrying danger-associated molecular pattern (DAMP) molecules
    Authors: GP Collett, CW Redman, IL Sargent, M Vatish
    Oncotarget, 2018-01-11;9(6):6707-6717.  2018-01-11
  15. Dexamethasone downregulates expression of carbonic anhydrase IX via HIF-1? and NF-?B-dependent mechanisms
    Int J Oncol, 2016-07-14;0(0):.  2016-07-14
  16. Profiling of cell stress protein expression in cardiac tissue of cardiosurgical patients undergoing remote ischemic preconditioning: implications for thioredoxin in cardioprotection.
    Authors: Zitta K, Meybohm P, Gruenewald M, Cremer J, Zacharowski K, Scholz J, Steinfath M, Albrecht M
    J Transl Med, 2015-01-27;13(0):34.  2015-01-27
  17. Changes and regulation of the C5a receptor on neutrophils during septic shock in humans.
    Authors: Unnewehr H, Rittirsch D, Sarma J, Zetoune F, Flierl M, Perl M, Denk S, Weiss M, Schneider M, Monk P, Neff T, Mihlan M, Barth H, Gebhard F, Ward P, Huber-Lang M
    J Immunol, 2013-03-11;190(8):4215-25.  2013-03-11


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Proteome Profiler Human Cell Stress Array Kit
By Anonymous on 12/11/2020

Proteome Profiler Human Cell Stress Array Kit
By Anonymous on 04/29/2020

Protein used 300 ug for each sample.

Proteome Profiler Human Cell Stress Array Kit
By Anonymous on 05/22/2018