Proteome Profiler Human Cell Stress Array Kit ARY018: R&D Systems
Kit Summary

A membrane-based antibody array for the parallel determination of the relative levels of selected human cell stress-related proteins. Validated for analyte detection in cell and tissue lysates.

Key Benefits

  • Detects 26 human cell stress-related proteins simultaneously
  • Requires no specialized equipment
 

Principle of the Array

The Proteome Profiler Human Cell Stress Array Kit is a membrane-based sandwich immunoassay. Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2). Captured proteins are visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.

Why Use an Antibody Array to Detect Cell Stress-Related Proteins?

Determining the expression of multiple cell stress-related proteins in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple proteins in a single sample can be cost-effective and also save time and sample.

 

Kit Contents
  • 4 Array Membranes
  • 4-Well Multi-dish
  • Array Buffers
  • Lysis Buffer
  • Wash Buffer
  • Detection Antibody Cocktail
  • Streptavidin-HRP
  • Chemiluminescent Detection Reagents
  • Transparency Overlay Template
  • Detailed Protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

Stability and Storage

Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.

 

 
Simultaneously detect the levels of these cell stress-related proteins in a single sample.
ADAMTS1 HIF-2 alpha Phospho-p38 alpha (T180/Y182)
Bcl-2 Phospho-HSP27 (S78/S82) Phospho-p53 (S46)
Carbonic Anhydrase IX HSP60 PON1
Cited-2 HSP70 PON2
COX-2 IDO PON3
Cytochrome c Phospho-JNK Pan (T183/Y185) Thioredoxin-1
Dkk-4 NFkappaB1 SIRT2
FABP1/L-FABP p21/CIP1 SOD2
HIF-1 alpha p27/Kip1  

 

Assays for analytes represented in the Human Cell Stress Array Kit

Analyte Quantkine® ELISA Kits DuoSet® ELISA Development Reagents DuoSet® IC ELISA Development Reagents Surveyor IC ELISA Kits Cell-Based ELISA Kits
ADAMTS1   DY2197      
Bcl-2     DYC827B    
Carbonic Anhydrase IX DCA900 DY2188      
Cited-2          
COX-2     DYC4198    
Cytochrome c DCTC0        
Dkk-4   DY1269      
FABP1/L-FABP Z-007        
HIF-1 alpha     DYC1935    
HIF-2 alpha          
Phospho-HSP27 (S78/S82)     DYC2314    
HSP60     DYC1800    
HSP70     DYC1663 DYC1663  
IDO          
Phospho-JNK Pan (T183/Y185)     DYC1387    
NFkappaB1          
p21/CIP1     DYC1047    
p27/Kip1     DYC2256    
Phospho-p38 alpha (T180/Y182)     DYC869B SUV869B  
Phospho-p53 (S46)     DYC1489    
PON1     DYC5816    
PON2     DYC4344    
PON3     DYC4345    
Thioredoxin-1          
SIRT2          
SOD2     DYC3419    

Troubleshooting Guide

Data Examples
HeLa human cervical epithelial carcinoma cells
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Figure 1: HeLa human cervical epithelial carcinoma cells were either untreated or exposed to 150 J/m2 of UV light followed by a 30 minute recovery period before lysis.

The Human Cell Stress Array
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Figure 2: The Human Cell Stress Array detects multiple analytes in tissue lysates. 300 µg of tissue lysate was run on each array. Data shown are from a 5 minute exposure to X-ray film.

Preparation and Storage
  • Stability & Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Cell Stress Array Kits
Protein kinases are the largest class of enzymes in the human genome. These enzymes regulate almost all cellular processes by adding phosphate groups to proteins, thereby modifying the activity, localization, and overall function of their targets. Consequently, abnormal activity of kinases underlies many diseases including cancer.
  • Alternate Names:
    Cell Stress Array Kits
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Species
Sample Type
  1. Dexamethasone downregulates expression of carbonic anhydrase IX via HIF-1? and NF-?B-dependent mechanisms
    Int J Oncol, 2016;0(0):. 2016
  2. Profiling of cell stress protein expression in cardiac tissue of cardiosurgical patients undergoing remote ischemic preconditioning: implications for thioredoxin in cardioprotection.
    Authors: Zitta K, Meybohm P, Gruenewald M, Cremer J, Zacharowski K, Scholz J, Steinfath M, Albrecht M
    J Transl Med, 2015;13(0):34. 2015
  3. Changes and regulation of the C5a receptor on neutrophils during septic shock in humans.
    Authors: Unnewehr H, Rittirsch D, Sarma J, Zetoune F, Flierl M, Perl M, Denk S, Weiss M, Schneider M, Monk P, Neff T, Mihlan M, Barth H, Gebhard F, Ward P, Huber-Lang M
    J Immunol, 2013;190(8):4215-25. 2013
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