Proteome Profiler Human Cell Stress Array Kit
Proteome Profiler Human Cell Stress Array Kit Summary
A membrane-based antibody array for the parallel determination of the relative levels of selected human cell stress-related proteins. Validated for analyte detection in cell and tissue lysates.
- Detects 26 human cell stress-related proteins simultaneously
- Requires no specialized equipment
Principle of the Array
The Proteome Profiler Human Cell Stress Array Kit is a membrane-based sandwich immunoassay. Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2). Captured proteins are visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.
Why Use an Antibody Array to Detect Cell Stress-Related Proteins?
Determining the expression of multiple cell stress-related proteins in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple proteins in a single sample can be cost-effective and also save time and sample.
- 4 Array Membranes
- 4-Well Multi-dish
- Array Buffers
- Lysis Buffer
- Wash Buffer
- Detection Antibody Cocktail
- Chemiluminescent Detection Reagents
- Transparency Overlay Template
- Detailed Protocol
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.
Stability and Storage
Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.
|Simultaneously detect the levels of these cell stress-related proteins in a single sample.|
|ADAMTS1||HIF-2 alpha||Phospho-p38 alpha (T180/Y182)|
|Bcl-2||Phospho-HSP27 (S78/S82)||Phospho-p53 (S46)|
|Carbonic Anhydrase IX||HSP60||PON1|
|Cytochrome c||Phospho-JNK Pan (T183/Y185)||Thioredoxin-1|
Assays for analytes represented in the Human Cell Stress Array Kit
|Analyte||Quantkine® ELISA Kits||DuoSet® ELISA Development Systems||DuoSet® IC ELISA Development Systems (Total)||DuoSet® IC ELISA Development Systems (Phospho)|
|Carbonic Anhydrase IX||DCA900||DY2188|
|Phospho-JNK Pan (T183/Y185)||DYC1387B||DYC2236|
|Phospho-p38 alpha (T180/Y182)||DYC8691B||DYC869B|
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Figure 1: HeLa human cervical epithelial carcinoma cells were either untreated or exposed to 150 J/m2 of UV light followed by a 30 minute recovery period before lysis.
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Figure 2: The Human Cell Stress Array detects multiple analytes in tissue lysates. 300 µg of tissue lysate was run on each array. Data shown are from a 5 minute exposure to X-ray film.
Preparation and Storage
Background: Cell Stress Array Kits
Protein kinases are the largest class of enzymes in the human genome. These enzymes regulate almost all cellular processes by adding phosphate groups to proteins, thereby modifying the activity, localization, and overall function of their targets. Consequently, abnormal activity of kinases underlies many diseases including cancer.
Citations for Proteome Profiler Human Cell Stress Array Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 4
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Endoplasmic reticulum stress stimulates the release of extracellular vesicles carrying danger-associated molecular pattern (DAMP) molecules
Authors: GP Collett, CW Redman, IL Sargent, M Vatish
Oncotarget, 2018;9(6):6707-6717. 2018
Dexamethasone downregulates expression of carbonic anhydrase IX via HIF-1? and NF-?B-dependent mechanisms
Int J Oncol, 2016;0(0):. 2016
Profiling of cell stress protein expression in cardiac tissue of cardiosurgical patients undergoing remote ischemic preconditioning: implications for thioredoxin in cardioprotection.
Authors: Zitta K, Meybohm P, Gruenewald M, Cremer J, Zacharowski K, Scholz J, Steinfath M, Albrecht M
J Transl Med, 2015;13(0):34. 2015
Changes and regulation of the C5a receptor on neutrophils during septic shock in humans.
Authors: Unnewehr H, Rittirsch D, Sarma J, Zetoune F, Flierl M, Perl M, Denk S, Weiss M, Schneider M, Monk P, Neff T, Mihlan M, Barth H, Gebhard F, Ward P, Huber-Lang M
J Immunol, 2013;190(8):4215-25. 2013
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