Proteome Profiler Human Cell Stress Array Kit

Contains 4 membranes - each spotted in duplicate with 26 different cell stress antibodies
Catalog # Availability Size / Price Qty
ARY018
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (1)

Proteome Profiler Human Cell Stress Array Kit Summary

Kit Summary

A membrane-based antibody array for the parallel determination of the relative levels of selected human cell stress-related proteins. Validated for analyte detection in cell and tissue lysates.

Troubleshooting Guide

Key Benefits

  • Detects 26 human cell stress-related proteins simultaneously
  • Requires no specialized equipment
 

Principle of the Array

The Proteome Profiler Human Cell Stress Array Kit is a membrane-based sandwich immunoassay. Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2). Captured proteins are visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.

Why Use an Antibody Array to Detect Cell Stress-Related Proteins?

Determining the expression of multiple cell stress-related proteins in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple proteins in a single sample can be cost-effective and also save time and sample.

 

Kit Contents
  • 4 Array Membranes
  • 4-Well Multi-dish
  • Array Buffers
  • Lysis Buffer
  • Wash Buffer
  • Detection Antibody Cocktail
  • Streptavidin-HRP
  • Chemiluminescent Detection Reagents
  • Transparency Overlay Template
  • Detailed Protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

Stability and Storage

Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.

 

 
Simultaneously detect the levels of these cell stress-related proteins in a single sample.
ADAMTS1 HIF-2 alpha Phospho-p38 alpha (T180/Y182)
Bcl-2 Phospho-HSP27 (S78/S82) Phospho-p53 (S46)
Carbonic Anhydrase IX HSP60 PON1
Cited-2 HSP70 PON2
COX-2 IDO PON3
Cytochrome c Phospho-JNK Pan (T183/Y185) Thioredoxin-1
Dkk-4 NFkappaB1 SIRT2
FABP1/L-FABP p21/CIP1 SOD2
HIF-1 alpha p27/Kip1  

 

Assays for analytes represented in the Human Cell Stress Array Kit

Analyte Quantkine® ELISA Kits DuoSet® ELISA Development Systems DuoSet® IC ELISA Development Systems (Total) DuoSet® IC ELISA Development Systems (Phospho)
ADAMTS1   DY2197    
Bcl-2     DYC827B  
Carbonic Anhydrase IX DCA900 DY2188    
Cited-2        
COX-2     DYC4198  
Cytochrome c DCTC0      
Dkk-4   DY1269    
FABP1/L-FABP        
HIF-1 alpha     DYC1935  
HIF-2 alpha        
Phospho-HSP27 (S78/S82)     DYC1580 DYC2314
HSP60     DYC1800  
HSP70   DY1663 DYC1663  
IDO   DY6030    
Phospho-JNK Pan (T183/Y185)     DYC1387B DYC2236
NFkappaB1        
p21/CIP1     DYC1047  
p27/Kip1     DYC2256  
Phospho-p38 alpha (T180/Y182)     DYC8691B DYC869B
Phospho-p53 (S46)     DYC1043  
PON1     DYC5816  
PON2        
PON3        
Thioredoxin-1        
SIRT2        
SOD2     DYC3419  

 

Data Examples
HeLa human cervical epithelial carcinoma cells
View Larger Image

Figure 1: HeLa human cervical epithelial carcinoma cells were either untreated or exposed to 150 J/m2 of UV light followed by a 30 minute recovery period before lysis.

The Human Cell Stress Array
View Larger Image

Figure 2: The Human Cell Stress Array detects multiple analytes in tissue lysates. 300 µg of tissue lysate was run on each array. Data shown are from a 5 minute exposure to X-ray film.

Product Datasheets

Preparation and Storage

Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Cell Stress Array Kits

Protein kinases are the largest class of enzymes in the human genome. These enzymes regulate almost all cellular processes by adding phosphate groups to proteins, thereby modifying the activity, localization, and overall function of their targets. Consequently, abnormal activity of kinases underlies many diseases including cancer.

Alternate Names
Cell Stress Array Kits

Citations for Proteome Profiler Human Cell Stress Array Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
Filter your results:

Filter by:

  1. Endoplasmic reticulum stress stimulates the release of extracellular vesicles carrying danger-associated molecular pattern (DAMP) molecules
    Authors: GP Collett, CW Redman, IL Sargent, M Vatish
    Oncotarget, 2018;9(6):6707-6717.  2018
  2. Dexamethasone downregulates expression of carbonic anhydrase IX via HIF-1? and NF-?B-dependent mechanisms
    Int J Oncol, 2016;0(0):.  2016
  3. Profiling of cell stress protein expression in cardiac tissue of cardiosurgical patients undergoing remote ischemic preconditioning: implications for thioredoxin in cardioprotection.
    Authors: Zitta K, Meybohm P, Gruenewald M, Cremer J, Zacharowski K, Scholz J, Steinfath M, Albrecht M
    J Transl Med, 2015;13(0):34.  2015
  4. Changes and regulation of the C5a receptor on neutrophils during septic shock in humans.
    Authors: Unnewehr H, Rittirsch D, Sarma J, Zetoune F, Flierl M, Perl M, Denk S, Weiss M, Schneider M, Monk P, Neff T, Mihlan M, Barth H, Gebhard F, Ward P, Huber-Lang M
    J Immunol, 2013;190(8):4215-25.  2013

FAQs

No product specific FAQs exist for this product, however you may

View all Proteome Profiler Antibody Array FAQs

Reviews for Proteome Profiler Human Cell Stress Array Kit

Average Rating: 5 (Based on 1 Review)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Proteome Profiler Human Cell Stress Array Kit?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Proteome Profiler Human Cell Stress Array Kit
By Anonymous on 05/22/2018