The Proteome Profiler Human Cell Stress Array Kit is a membrane-based sandwich immunoassay. Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2). Captured proteins are visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.
Determining the expression of multiple cell stress-related proteins in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple proteins in a single sample can be cost-effective and also save time and sample.
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.
Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.
|Simultaneously detect the levels of these cell stress-related proteins in a single sample.|
|ADAMTS1||HIF-2 alpha||Phospho-p38 alpha (T180/Y182)|
|Bcl-2||Phospho-HSP27 (S78/S82)||Phospho-p53 (S46)|
|Carbonic Anhydrase IX||HSP60||PON1|
|Cytochrome c||Phospho-JNK Pan (T183/Y185)||Thioredoxin-1|
|Analyte||Quantkine® ELISA Kits||DuoSet® ELISA Development Reagents||DuoSet® IC ELISA Development Reagents||Surveyor™ IC ELISA Kits||Cell-Based ELISA Kits|
|Carbonic Anhydrase IX||DCA900||DY2188|
|Phospho-JNK Pan (T183/Y185)||DYC1387|
|Phospho-p38 alpha (T180/Y182)||DYC869B||SUV869B|
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Figure 1: HeLa human cervical epithelial carcinoma cells were either untreated or exposed to 150 J/m2 of UV light followed by a 30 minute recovery period before lysis.
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Figure 2: The Human Cell Stress Array detects multiple analytes in tissue lysates. 300 µg of tissue lysate was run on each array. Data shown are from a 5 minute exposure to X-ray film.
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