Rat CCR6 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB8320R-100
MAB8320R-SP
Detection of CCR6 in HEK293 Human Cell Line Transfected with Rat CCR6  and eGFP by Flow Cytometry
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Rat CCR6 Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat CCR6 in direct ELISAs.
Source
Recombinant Monoclonal Mouse IgG2B Clone # 876515R
Purification
Protein A or G purified from cell culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with rat CCR6
Met1-Met366
Accession # Q5BK58
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CCR6 in HEK293 Human Cell Line Transfected with Rat CCR6  and eGFP by Flow Cytometry View Larger

Detection of CCR6 in HEK293 Human Cell Line Transfected with Rat CCR6 and eGFP by Flow Cytometry HEK293 human embryonic kidney cell line transfected with either (A) rat CCR6 or (B) irrelevant protein and eGFP was stained with Mouse Anti-Rat CCR6 Monoclonal Antibody (Catalog # MAB8320R) followed by APC-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on Mouse IgG2b isotype control antibody staining (Catalog # MAB0041, data not shown).  View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR6

CCR6 is an approximately 45 kDa G-protein linked seven transmembrane domain receptor that binds the chemokine CCL20/MIP-3 alpha. Among hematopoietic cells, CCR6 is expressed on B cells, immature dendritic cells, neutrophils, and various T cell subsets (e.g. CD4+ T cells, regulatory T cells, effector memory Th17 cells, and g/d T cells). It is upregulated in several cancers, Alzheimer’s brain, and the airways in obstructive pulmonary disease. CCR6 plays an important role in mucosal immunity, IgE mediated allergic reactions, immune cell infiltration into sites of inflammation, and tumor cell invasiveness. It also promotes chemotaxis and tumor angiogenesis in response to beta defensins. Rat CCR6 shares 74% and 87% amino acid sequence identity with human and mouse CCR6, respectively.

Entrez Gene IDs
1235 (Human); 12458 (Mouse); 308163 (Rat)
Alternate Names
BN-1; CC-CKR-6; CCR6; CCR-6; CD196 antigen; CD196; chemokine (C-C motif) receptor 6; CKR-L3G-protein coupled receptor 29; CKRL3LARC receptor; CMKBR6STRL22; DRY6; GPR29; GPR29C-C CKR-6; GPR-CY4; GPRCY4DRY-6; lymphocyte, 22; STRL22

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2B Antibody

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