Detects CXCL7/Thymus Chemokine‑1 in direct ELISAs and Western blots. In direct ELISAs,
approximately 10% cross-reactivity with recombinant mouse CXCL7 is observed and less than 1% cross-reactivity with _______________________ is observed.
Polyclonal Goat IgG
E. coli-derived recombinant rat CXCL7/Thymus Chemokine-1 Ile46-Ile107 Accession # NP_714943
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Recombinant Rat CXCL7/Thymus Chemokine‑1 (Catalog # 1116-CK)
Perfusion fixed frozen sections of rat thymus
Measured by its ability to neutralize CXCL7/Thymus Chemokine‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 0.8-4.0 µg/mL in the presence of 0.04 µg/mL Recombinant Rat CXCL7/Thymus Chemokine‑1.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Chemotaxis Induced by CXCL7/Thymus Chemokine‑1 and Neutralization by Rat CXCL7/Thymus Chemokine‑1 Antibody. Recombinant Rat CXCL7/Thymus Chemokine‑1 (Catalog # 1116-CK) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CXCL7/Thymus Chemokine‑1 (0.04 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat CXCL7/Thymus Chemokine‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1116). The ND50 is typically 0.8-4.0 µg/mL.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL7/Thymus Chemokine-1
Thymus chemokine-1 (TCK-1) is a member of the CXC subfamily of chemokines. Mouse TCK-1 shares 72% amino acid sequence identity with rat TCK-1.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.