Rat Fas Ligand/TNFSF6 Antibody Summary
Accession # P36940
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Fas Ligand/TNFSF6 in Rat Splenocytes. Fas Ligand/TNFSF6 was detected in immersion fixed rat splenocytes using Goat Anti-Rat Fas Ligand/TNFSF6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1858) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Fas Ligand/TNFSF6
Fas Ligand (FasL), also known as CD178, CD95L, or TNFSF6, is a 40 kDa type II transmembrane member of the TNF superfamily of proteins. Its ability to induce apoptosis in target cells plays an important role in the development, homeostasis, and function of the immune system (1). Mature rat Fas Ligand consists of a 179 amino acid (aa) extracellular domain (ECD), a 22 aa transmembrane segment, and a 79 aa cytoplasmic domain. Within the ECD, rat Fas Ligand shares 78% and 93% aa sequence identity with human and mouse Fas Ligand, respectively. Fas Ligand is expressed as a nondisulfide-linked homotrimer on the surface of activated CD4+ Th1 cells, CD8+ cytotoxic T cells, and NK cells (1). Fas Ligand binding to Fas/CD95 on an adjacent cell triggers apoptosis in the Fas‑expressing cell (2, 4). Fas Ligand also binds DcR3 which is a soluble decoy receptor that interferes with Fas Ligand-induced apoptosis (5). Fas Ligand can be released from the cell surface by metalloproteinases as a 26 kDa soluble molecule which remains trimeric (6, 7). Shed Fas Ligand retains the ability to bind Fas, although its ability to trigger apoptosis is dramatically reduced (6, 7). In the absence of TGF‑ beta, however, Fas Ligand/Fas interactions instead promote neutrophil-mediated inflammatory responses (3, 8). Fas Ligand itself transmits reverse signals that costimulate the proliferation of freshly antigen-stimulated T cells (9). Fas Ligand-induced apoptosis plays a central role in the development of immune tolerance and the maintance of immune privileged sites (10). This function is exploited by tumor cells which evade immune surveillance by upregulating Fas Ligand to kill tumor infiltrating lymphocytes (8, 11). In gld mice, a Fas Ligand point mutation is the cause of severe lymphoproliferation and systemic autoimmunity (12, 13).
- Lettau, M. et al. (2008) Curr. Med. Chem. 15:1684.
- Takahashi, T. et al. (1994) Int. Immunol. 6:1567.
- Seino, K-I. et al. (1998) J. Immunol. 161:4484.
- Suda, T. et al. (1993) Cell 75:1169.
- Pitti, R.M. et al. (1998) Nature 396:699.
- Schneider, P. et al. (1998) J. Exp. Med. 187:1205.
- Tanaka, M. et al. (1998) Nature Med. 4:31.
- Chen, J.-J. et al. (1998) Science 282:1714.
- Suzuki, I. and P.J. Fink (2000) Proc. Natl. Acad. Sci. 97:1707.
- Ferguson, T.A. and T.S. Griffith (2006) Immunol. Rev. 213:228.
- Ryan, A.E. et al. (2005) Cancer Res. 65:9817.
- Takahashi, T. et al. (1994) Cell 76:969.
- Lynch, D.H. et al. (1994) Immunity 1:131.
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Cell and Tissue Staining Kits
Reviews for Rat Fas Ligand/TNFSF6 Antibody
There are currently no reviews for this product. Be the first to review Rat Fas Ligand/TNFSF6 Antibody and earn rewards!
Have you used Rat Fas Ligand/TNFSF6 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image