Rat Hepassocin/FGL1 Antibody

Catalog # Availability Size / Price Qty
MAB11052-100
MAB11052-SP
Rat Hepassocin/FGL1 Antibody in ELISA
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Rat Hepassocin/FGL1 Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat Hepassocin/FGL1 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 160902
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat Hepassocin/FGL1
Accession # Q5M8C6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Rat Hepassocin/FGL1 Monoclonal Antibody (Catalog # MAB11051).

This product is intended for assay development on various assay platforms requiring antibody pairs.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Rat Hepassocin/FGL1 ELISA Standard Curve. Recombinant Rat Hepassocin/FGL1 protein was serially diluted 2-fold and captured by Mouse Anti-Rat Hepassocin/FGL1 Monoclonal Antibody (Catalog # MAB11052) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Rat Hepassocin/FGL1Monoclonal Antibody (Catalog # MAB11051) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Hepassocin/FGL1

Hepassocin, also known as hepatocyte-derived fibrinogen-related protein 1 (HFREP-1), is a liver-specific secreted protein containing one fibrinogen C-terminal domain.

Long Name
Fibrinogen Like 1
Entrez Gene IDs
2267 (Human); 246186 (Rat)
Alternate Names
FGL1; fibrinogen-like 1; fibrinogen-like protein 1; Fibrinogen-like-protein 1; FREP1; Hepassocin; hepatocellular carcinoma-related sequence; Hepatocyte-derived fibrinogen-related protein 1; HFREP-1; HFREP1MGC12455; HP-041; LFIRE1; LFIRE-1; Liver fibrinogen-related protein 1

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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