Rat IL-12 Antibody Summary
p40: Met23-Ser335; p35: Arg23-Ser215
Accession # p40: NP_072133; p35: Q9R103
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by IL‑12 and Neutralization by Rat IL‑12 Antibody. Recombinant Rat IL-12 (Catalog # 1760-RL) stimulates proliferation in mouse splenocytes in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat IL-12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1760). The ND50is typically 0.05-0.25 µg/mL.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Interleukin 12 (IL-12) is the founding member of the IL-12 family of heterodimeric cytokines, which have important immunological functions. IL-12 is composed of two disulfide-linked subunits of 35 kDa and 40 kDa, respectively. The 35 kDa subunit (p35) is an alpha -helical protein homologous to IL-6 and G‑CSF. The 40 kDa subunit (p40) contains one fibronectin type III and one Ig C2-like domain, and has a high degree of structural homology to type I cytokine receptors. Whereas p35 subunit is unique to IL-12, the p40 subunit is also utilized in IL-23. Mature rat p35 is a 194 amino acids (aa) protein that is secreted as a heterodimer linked to p40. It contains three potential N-linked glycosylation sites and shares 86%, and 58% aa sequence identity with mouse and human p35, respectively. Mature rat p40 contains 313 aa and can exist in multiple forms, including monomer, homodimer, heterodimer linked to p19 (forming IL-23), and heterodimer linked to p35 (forming IL-12). Mature rat p40 shows 92% and 66% aa sequence identity to mouse and human p40, respectively. Cells known to produce IL-12 include macrophages, dendritic cells, monocytes, Langerhans cells, neutrophils, and keratinocytes. The activities of IL-12 are mediated by the receptor complex composed of two type I transmembrane proteins: a 100 kDa ligand-binding subunit (IL‑12 R beta 1) and a 130 kDa signal transducing subunit (IL‑12 R beta 2). IL‑12 facilitates hematopoietic stem cell proliferation, induces NK cell proliferation, and potentiates the expansion and late activation of Th1 CD4+ T cells (1-4).
- Park, A.Y. and P. Scott (2001) Scand. J. Immunol. 53:529.
- Trinchieri, G. et al. (2003) Immunity 19:641.
- Brombacher, F. et al. (2003) Trends Immunol. 24:207.
- Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49.
Citation for Rat IL-12 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Semliki Forest virus-mediated gene therapy of the RG2 rat glioma.
Authors: Roche FP, Sheahan BJ, O'Mara SM, Atkins GJ
Neuropathol. Appl. Neurobiol., 2010;36(7):648-60.
Sample Types: Whole Tissue
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