Rat IL-12 Antibody

Catalog # Availability Size / Price Qty
AF1760
AF1760-SP
Cell Proliferation Induced by IL‑12 and Neutralization by Rat IL‑12 Antibody.
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Product Details
Citations (1)
FAQs
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Reviews (1)

Rat IL-12 Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat IL-12 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse IL‑12 and less than 5% cross-reactivity with recombinant human IL-12 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant rat IL‑12
p40: Met23-Ser335; p35: Arg23-Ser215
Accession # p40: NP_072133; p35: Q9R103
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.30 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Rat IL‑12 (Catalog # 1760-RL)
Neutralization
Measured by its ability to neutralize IL‑12-induced proliferation in mouse splenocytes. The Neutralization Dose (ND50) is typically 0.05-0.25 µg/mL in the presence of 1 ng/mL Recombinant Rat IL‑12. This antibody will also neutralize rrIL-23 activity.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization Cell Proliferation Induced by IL‑12 and Neutralization by Rat IL‑12 Antibody. View Larger

Cell Proliferation Induced by IL‑12 and Neutralization by Rat IL‑12 Antibody.

Recombinant Rat IL‑12 (Catalog # 1760-RL) stimulates proliferation in mouse splenocytes in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL‑12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat IL‑12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1760). The ND50 is typically
0.05-0.25 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-12

Interleukin 12 (IL-12) is the founding member of the IL-12 family of heterodimeric cytokines, which have important immunological functions. IL-12 is composed of two disulfide-linked subunits of 35 kDa and 40 kDa, respectively. The 35 kDa subunit (p35) is an alpha -helical protein homologous to IL-6 and G‑CSF. The 40 kDa subunit (p40) contains one fibronectin type III and one Ig C2-like domain, and has a high degree of structural homology to type I cytokine receptors. Whereas p35 subunit is unique to IL-12, the p40 subunit is also utilized in IL-23. Mature rat p35 is a 194 amino acids (aa) protein that is secreted as a heterodimer linked to p40. It contains three potential N-linked glycosylation sites and shares 86%, and 58% aa sequence identity with mouse and human p35, respectively. Mature rat p40 contains 313 aa and can exist in multiple forms, including monomer, homodimer, heterodimer linked to p19 (forming IL-23), and heterodimer linked to p35 (forming IL-12). Mature rat p40 shows 92% and 66% aa sequence identity to mouse and human p40, respectively. Cells known to produce IL-12 include macrophages, dendritic cells, monocytes, Langerhans cells, neutrophils, and keratinocytes. The activities of IL-12 are mediated by the receptor complex composed of two type I transmembrane proteins: a 100 kDa ligand-binding subunit (IL‑12 R beta 1) and a 130 kDa signal transducing subunit (IL‑12 R beta 2). IL‑12 facilitates hematopoietic stem cell proliferation, induces NK cell proliferation, and potentiates the expansion and late activation of Th1 CD4+ T cells (1-4).

References
  1. Park, A.Y. and P. Scott (2001) Scand. J. Immunol. 53:529.
  2. Trinchieri, G. et al. (2003) Immunity 19:641.
  3. Brombacher, F. et al. (2003) Trends Immunol. 24:207.
  4. Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49.
Long Name
Interleukin 12
Entrez Gene IDs
3592 (Human); 16159 (Mouse); 84405 (Rat); 403977 (Canine); 493741 (Feline)
Alternate Names
CLMF p35; CLMF1; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL-12 subunit p35; IL12; IL-12; IL-12, subunit p35; IL12A; interleukin 12, p35; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35); interleukin-12 alpha chain; interleukin-12 subunit alpha; natural killer cell stimulatory factor 1, 35 kD subunit; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; NKSF1; p35

Product Datasheets

Citation for Rat IL-12 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Semliki Forest virus-mediated gene therapy of the RG2 rat glioma.
    Authors: Roche FP, Sheahan BJ, O'Mara SM, Atkins GJ
    Neuropathol. Appl. Neurobiol., 2010;36(7):648-60.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC Frozen

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Rat IL-12 Antibody
By Anonymous on 11/08/2017
Application: ELISA Sample Tested: Serum Species: Rat

This antibody was used to build an ELISA to measure rat IL-12. The antibody served well as both the capture and detection in a sandwich ELISA format. Rat Il-12 was measured in serum samples.