Rat RAGE Antibody

Catalog # Availability Size / Price Qty
AF1616
AF1616-SP
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (1)

Rat RAGE Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat RAGE in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human RAGE and recombinant canine RAGE is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat RAGE
Gln24-Ala342
Accession # Q63495
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Rat RAGE Fc Chimera (Catalog # 1616-RG)
Blockade of Receptor-ligand Interaction
In a functional ELISA, 5-25 µg/mL of this antibody will block 50% of the binding of 5 μg/mL of biotinylated AGE-BSA to immobilized Recombinant Rat RAGE Fc Chimera (Catalog # 1616-RG) coated at 5 µg/mL (100 µL/well). At 166 μg/mL, this antibody will block >90% of the binding.
 

Rat RAGE Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Rat RAGE Biotinylated Antibody (Catalog # BAF1616)

Standard: Recombinant Rat RAGE Fc Chimera Protein, CF (Catalog # 1616-RG)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RAGE/AGER

Advanced glycation endproducts (AGE) are adducts formed by the non-enzymatic glycation or oxidation of macromolecules (1). AGE forms during aging and its formation is accelerated under pathophysiologic states such as diabetes, Alzheimer’s disease, renal failure and immune/inflammatory disorders. Receptor for Advanced Glycation Endoproducts (RAGE), named for its ability to bind AGE, is a multiligand receptor belonging to the immunoglobulin (Ig) superfamily. Besides AGE, RAGE binds amyloid beta -peptide, S100/calgranulin family proteins, high mobility group B1 (HMGB1, also know as amphoterin) and leukocyte integrins (1, 2).

The human RAGE gene encodes a 404 amino acid (aa) residues type I transmembrane glycoprotein with a 22 aa signal peptide, a 319 aa extracellular domain containing an Ig-like V-type domain and two Ig-like Ce-type domains, a 21 aa transmembrane domain and a 40 aa cytoplasmic domain (3). The V-type domain and the cytoplasmic domain are important for ligand binding and for intracellular signaling, respectively. Two alternative splice variants, lacking the V‑type domain or the cytoplasmic tail, are known (1, 4). RAGE is highly expressed in the embryonic central nervous system (5). In adult tissues, RAGE is expressed at low levels in multiple tissues including endothelial and smooth muscle cells, mononuclear phagocytes, pericytes, microglia, neurons, cardiac myocytes and hepatocytes (6). The expression of RAGE is upregulated upon ligand interaction. Depending on the cellular context and interacting ligand, RAGE activation can trigger differential signaling pathways that affect divergent pathways of gene expression (1, 7). RAGE activation modulates varied essential cellular responses (including inflammation, immunity, proliferation, cellular adhesion and migration) that contribute to cellular dysfunction associated with chronic diseases such as diabetes, cancer, amyloidoses and immune or inflammatory disorders (1).

References
  1. Schmidt, A. et al. (2001) J. Clin. Invest. 108:949.
  2. Chavakis, T. et al. (2003) J. Exp. Med. 198:507.
  3. Neeper, M. et al. (1992) J. Biol. Chem. 267:14998.
  4. Yonekura, H. et al. (2003) Biochem. J. 370:1097.
  5. Hori, O. et al. (1995) J. Biol. Chem. 270:25752.
  6. Brett, J. et al. (1993) Am. J. Pathol. 143:1699.
  7. Valencia, J.V. et al. (2004) Diabetes 53:743.
Long Name
Receptor for Advanced Glycation End Products
Entrez Gene IDs
177 (Human); 11596 (Mouse); 81722 (Rat); 403168 (Canine)
Alternate Names
advanced glycosylation end product-specific receptor; AGER; RAGE isoform delta; RAGE isoform sRAGE-delta; RAGE; Receptor for advanced glycosylation end products; receptor for advanced glycosylation end-products; SCARJ1

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Citations for Rat RAGE Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Reduction of advanced-glycation end products levels and inhibition of RAGE signaling decreases rat vascular calcification induced by diabetes.
    Authors: Brodeur M, Bouvet C, Bouchard S, Moreau S, Leblond J, Deblois D, Moreau P
    PLoS ONE, 2014;9(1):e85922.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Hypoxia-inducible factor-1 mediates neuronal expression of the receptor for advanced glycation end products following hypoxia/ischemia.
    Authors: Pichiule P, Chavez JC, Schmidt AM, Vannucci SJ
    J. Biol. Chem., 2007;282(50):36330-40.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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Rat RAGE Antibody
By Anonymous on 02/24/2016
Application: B/N Sample Tested: Spleen tissue Species: Rat