Key Product Details

Species Reactivity

Validated:

Rat

Cited:

Rat

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 442506
View all TSH beta Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant rat TSH beta
Phe21-Gly138
Accession # P04652

Specificity

Detects rat TSH beta in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows approximately 5% cross‑reactivity with recombinant human TSH beta.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Rat TSH  beta Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Rat TSH  beta

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TSH beta

TSH beta is the 138 amino acid (aa), 15 kDa unique subunit of Thyroid Stimulating Hormone (TSH), which shares a common alpha subunit with other glycoprotein hormones lutropin (LH), follitropin (FSH) and chorionic gonadotropin (CG). Production of TSH by the anterior pituitary gland is stimulated by the hypothalamic peptide TRH, and inhibited by feedback from the thyroid hormones triiodothyronine (T3) and thyroxine (T4). Mature rat TSH beta shares 92% and 90% aa sequence identity with mouse and human TSH beta, respectively.

Long Name

Thyroid-stimulating Hormone Subunit beta

Alternate Names

Thyrotropin beta, TSHB

Entrez Gene IDs

7252 (Human); 22094 (Mouse); 25653 (Rat)

Gene Symbol

TSHB

UniProt

P04652

Additional TSH beta Products

Product Documents for Rat TSH  beta Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Rat TSH  beta Antibody

For research use only

Citations for Rat TSH  beta Antibody

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FAQs

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