Rat UNC5H2/UNC5B Antibody

Catalog # Availability Size / Price Qty
MAB1006
MAB1006-SP
Product Details
Citations (1)
FAQs
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Rat UNC5H2/UNC5B Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat UNC5H2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant rat UNC5H1 or recombinant human UNC5H4 is observed.
Source
Monoclonal Mouse IgG2A Clone # 187305
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat UNC5H2
Gly27-Asp373
Accession # O08722
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Rat UNC5H2 Fc Chimera (Catalog # 1006-UN)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: UNC5H2/UNC5B

Caenorhabditis elegans UNC5 (UNC = behaviorally uncoordinated) and its mammalian homologues (including rat UNC5H1 and H2, mouse UNC5H2 and H3 (also known as rostral cerebellar malformation, RCM), and human UNC5H3 and H4) are transmembrane proteins belonging to the immunoglobulin (Ig) superfamily. All UNC5 family members have two Ig and two thrombospondin type 1 domains in their extracellular regions, as well as a conserved ZU-5 domain, a DCC (Deleted in Colorectal Cancer)-binding domain (DB) and a C-terminal death domain (DD) in their cytoplasmic regions (1, 2). UNC5 family proteins are receptors for the netrin/UNC6 (netr: Sanskrit for “one who guides”) family of secreted axon guidance cues that are laminin-related proteins. Netrin family proteins can act as a chemoattractant for some axons and as a chemorepellent for others. Besides UNC5, netrin family proteins also bind to the DCC family of type I transmembrane receptors that share sequence similarity with proteins of the NCAM family, and adenosine A2b receptor, a G protein-coupled seven‑transmembrane receptor belonging to the adenosine receptor family (3, 4). In vitro, netrin binding to DCC family receptors in the absence of UNC5 is associated with axon attraction. However, the DCC-mediated attraction to netrin is converted to repulsion by binding of UNC5 to the DCC-netrin complex. In vivo, the mechanisms of netrin-dependent axon attraction and repulsion are more complex and may include UNC5-mediated repulsion that is independent of DCC (1, 5). Besides their roles in axon guidance and neuronal migration, the UNC5 and DCC families also act as dependence receptors and exert pro-apoptotic effects in the absence of netrin (6). Rat UNC5H2 cDNA encodes a 945 amino acid (aa) type I membrane protein with a putative 26 aa signal peptide and 347 aa extracellular domain. The extracellular domain of rat UNC5H2 shares approximately 65%, 73% and 73% aa sequence similarity with that of rat UNC5H1, human UNC5H3 and mouse UNC5H3, respectively.

References
  1. Hong, K. et al. (1999) Cell 97:927.
  2. Leonardo, E.D. et al. (1997) Nature 386:833.
  3. Culotti, J.B. and D.C. Merz (1998) Curr. Opin. Cell Biol. 10:609.
  4. Corset, V. (2000) Nature 407:747.
  5. Merz, D.C. (2001) Genetics 158:1071.
  6. Llambi, F. et al. (2001) EMBO J. 20:2715.
Long Name
Netrin Receptor UNC5B
Entrez Gene IDs
219699 (Human); 60630 (Rat)
Alternate Names
p53RDL1; p53-regulated receptor for death and life protein 1; Protein unc-5 homolog 2; Protein unc-5 homolog B; transmembrane receptor Unc5H2; unc5 (C.elegans homolog) b; unc-5 homolog 2; unc-5 homolog B (C. elegans); UNC5B; UNC5H2; UNC5H2netrin receptor UNC5B

Product Datasheets

Citation for Rat UNC5H2/UNC5B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Netrin-4 enhances angiogenesis and neurologic outcome after cerebral ischemia.
    Authors: Hoang S, Liauw J, Choi M
    J. Cereb. Blood Flow Metab., 2009;29(2):385-97.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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