Recombinant Human/Mouse FGF-8b Protein, CF

(33 citations)   
  • Purity
    >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.01 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is typically 6.5-40 ng/mL in the presence of 1 µg/mL heparin.
  • Source
    E. coli-derived Gln23-Arg215, with an N-terminal Met
  • Accession #
  • N-terminal Sequence
    Analysis
    Met
  • Predicted Molecular Mass
    22.5 kDa
  • SDS-PAGE
    23 kDa, reducing condtions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
423-F8/CF
 
423-F8
Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4 and Brij-35.
Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4 and Brij-35 with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Reconstitution Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, 2 to 8 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, 2 to 8 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human/Mouse FGF-8b (Catalog # 423-F8/CF) stimulates cell proliferation in the NR6R‑3T3 mouse fibroblast cell line. The ED50 for this effect is 6.5-40 ng/mL in the presence of 1 μg/mL heparin.
1 μg/lane of Recombinant Human/Mouse FGF-8b was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 23 kDa.
Background: FGF-8

FGF-8 is a member of the fibroblast growth factor family that was originally discovered as a growth factor essential for the androgen-dependent growth of mouse mammary carcinoma cells (1-3). Alternate splicing of mouse FGF-8 mRNA generates eight secreted isoforms, designated a-h, but only FGF-8a, b, e and f exist in humans (4). FGF-8 contains a 22 amino acid (aa) signal sequence, an N‑terminal domain that varies according to the isoform (30 aa for FGF-8b; 20 aa for the shortest, FGF-8a), a 125 aa FGF domain and a 37 aa proline‑rich C‑terminal sequence. The FGF domain of FGF-8 shares the most aa identity with FGF17 (75%) and FGF-18 (67%), and the three form an FGF subfamily (2). Mouse FGF-8b shares 100% aa identity with human FGF-8b. FGF-8 is widely expressed during embryogenesis, and mediates epithelial-mesenchymal transitions. It plays an organizing and inducing role during gastrulation, and regulates patterning of the midbrain/hindbrain, eye, ear, limbs and heart in the embryo (2, 5 - 8). The isoforms may play different roles in development. FGF-8b shows the strongest receptor affinity and oncogenic transforming capacity although FGF-8a and FGF-8e are also transforming and have been found in human prostate, breast or ovarian tumors (1, 5, 9-12). FGF-8 shows limited expression in the normal adult, but low levels are found in the reproductive and genitourinary tract, peripheral leukocytes and bone marrow hematopoietic cells (3, 9, 13).

  • References:
    1. Mattila, M.M. and P.L. Harkonen (2007) Cytokine Growth Factor Rev. 18:257.
    2. Reuss, B. and O. von Bohlen und Halbach (2003) Cell Tiss. Res. 313:139.
    3. Tanaka, A. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8928.
    4. Gemel, J. et al. (1996) Genomics 35:253.
    5. Olsen, S.K. et al. (2006) Genes Dev. 20:185.
    6. Crossley, P.H. et al. (1996) Cell, 84:127.
    7. Heikinheimo, M. et al. (1994) Mech. Dev. 48:129.
    8. Sun, X. et al. (1999) Genes Dev. 13:1834.
    9. Ghosh, A.K. et al. (1996) Cell Growth Differ. 7:1425.
    10. Mattila, M.M. et al. (2001) Oncogene 20:2791.
    11. Valve, E. et al. (2000) Int. J. Cancer 88:718.
    12. Valve, E.M. et al. (2001) Lab. Invest. 81:815.
    13. Nezu, M. et al. (2005) Biochem. Biophys. Res. Commun. 335:843.
  • Long Name:
    Fibroblast Growth Factor 8
  • Entrez Gene IDs:
    2253 (Human); 14179 (Mouse); 29349 (Rat)
  • Alternate Names:
    AIGF; AIGFKAL6; Androgen-induced growth factor; FGF8; FGF-8; fibroblast growth factor 8 (androgen-induced); fibroblast growth factor 8; HBGF-8; Heparin-binding growth factor 8; MGC149376
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

33 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Integration of Shh and Fgf signaling in controlling Hox gene expression in cultured limb cells
    Authors: AR Rodrigues, N Yakushiji-, Y Atsuta, G Andrey, P Schorderet, D Duboule, CJ Tabin
    Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):.
    Species: Chicken
    Sample Type: Whole Cells
    Application: Bioassay
  2. Neurotoxic reactive astrocytes are induced by activated microglia
    Authors: SA Liddelow, KA Guttenplan, LE Clarke, FC Bennett, CJ Bohlen, L Schirmer, ML Bennett, AE Mnch, WS Chung, TC Peterson, DK Wilton, A Frouin, BA Napier, N Panicker, M Kumar, MS Buckwalter, DH Rowitch, VL Dawson, TM Dawson, B Stevens, BA Barres
    Nature, 2017;541(7638):481-487.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  3. N-Acetyl Cysteine May Support Dopamine Neurons in Parkinson's Disease: Preliminary Clinical and Cell Line Data
    Authors: Daniel A Monti
    PLoS ONE, 2016;11(6):e0157602.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  4. Fibulin-1 Binds to Fibroblast Growth Factor 8 with High Affinity: Effects on Embryo Survival
    J Biol Chem, 2016;0(0):.
    Species: Human
    Sample Type: Protein
    Application: Bioassay
  5. Dopamine Receptor Antagonists Enhance Proliferation and Neurogenesis of Midbrain Lmx1a-expressing Progenitors
    Authors: Eva Hedlund
    Sci Rep, 2016;6(0):26448.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  6. 2-O Heparan Sulfate Sulfation by Hs2st Is Required for Erk/Mapk Signalling Activation at the Mid-Gestational Mouse Telencephalic Midline.
    Authors: Chan W, Howe K, Clegg J, Guimond S, Price D, Turnbull J, Pratt T
    PLoS ONE, 2015;10(6):e0130147.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  7. Collective cell migration of the nephric duct requires FGF signaling.
    Authors: Attia L, Schneider J, Yelin R, Schultheiss T
    Dev Dyn, 2015;244(2):157-67.
    Species: Chicken
    Sample Type: In Vivo
    Application: Bioassay
  8. Manipulating gene expression and signaling activity in cultured mouse limb bud cells.
    Authors: Lewandowski J, Pursell T, Rabinowitz A, Vokes S
    Dev Dyn, 2014;243(7):928-36.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  9. Etv1 and Ewsr1 cooperatively regulate limb mesenchymal Fgf10 expression in response to apical ectodermal ridge-derived fibroblast growth factor signal.
    Authors: Yamamoto-Shiraishi Y, Higuchi H, Yamamoto S, Hirano M, Kuroiwa A
    Dev Biol, 2014;394(1):181-90.
    Species: Chicken
    Sample Type: Whole Cells
    Application: Bioassay
  10. Transcription factor-induced lineage programming of noradrenaline and motor neurons from embryonic stem cells.
    Authors: Mong J, Panman L, Alekseenko Z, Kee N, Stanton L, Ericson J, Perlmann T
    Stem Cells, 2014;32(3):609-22.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  11. Susceptibility of human embryonic stem cell-derived neural cells to Japanese encephalitis virus infection.
    Authors: Shen, Shih-Che, Shen, Ching-I, Lin, Ho, Chen, Chun-Jun, Chang, Chia-Yu, Chen, Sheng-Me, Lee, Hsiu-Chi, Lai, Ping-Sha, Su, Hong-Lin
    PLoS ONE, 2014;9(12):e114990.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  12. Self-assembling peptide nanofiber scaffolds enhance dopaminergic differentiation of mouse pluripotent stem cells in 3-dimensional culture.
    Authors: Ni, Na, Hu, Yaohua, Ren, Huixia, Luo, Chuanmin, Li, Peng, Wan, Jian-Bo, Su, Huanxing
    PLoS ONE, 2013;8(12):e84504.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  13. Fgf3 and Fgf10a work in concert to promote maturation of the epibranchial placodes in zebrafish.
    Authors: McCarroll, Matthew, Nechiporuk, Alex V
    PLoS ONE, 2013;8(12):e85087.
    Species: Zebrafish
    Sample Type: Whole Cells
    Application: In Vivo
  14. MicroRNA-based promotion of human neuronal differentiation and subtype specification.
    Authors: Stappert L, Borghese L, Roese-Koerner B, Weinhold S, Koch P, Terstegge S, Uhrberg M, Wernet P, Brustle O
    PLoS ONE, 2013;8(3):e59011.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  15. Human embryonic and rat adult stem cells with primitive endoderm-like phenotype can be fated to definitive endoderm, and finally hepatocyte-like cells.
    Authors: Roelandt P, Pauwelyn KA, Sancho-Bru P
    PLoS ONE, 2010;5(8):e12101.
    Species: Rat
    Sample Type: Whole Cells
    Application: Expansion/Differentiation
  16. Altered splicing of FGFR1 is associated with high tumor grade and stage and leads to increased sensitivity to FGF1 in bladder cancer.
    Authors: Tomlinson DC, Knowles MA
    Am. J. Pathol., 2010;177(5):2379-86.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  17. FGFs, Wnts and BMPs mediate induction of VEGFR-2 (Quek-1) expression during avian somite development.
    Authors: Nimmagadda S, Geetha-Loganathan P, Scaal M, Christ B, Huang R
    Dev. Biol., 2007;305(2):421-9.
    Species: Avian - Quail
    Sample Type: In Vivo
    Application: In Vivo
  18. Differentiation of ES cells into cerebellar neurons.
    Authors: Salero E, Hatten ME
    Proc. Natl. Acad. Sci. U.S.A., 2007;104(8):2997-3002.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  19. Cdx-Hox code controls competence for responding to Fgfs and retinoic acid in zebrafish neural tissue.
    Authors: Shimizu T, Bae YK, Hibi M
    Development, 2006;133(23):4709-19.
    Species: Zebrafish
    Sample Type: Whole Tissue
    Application: Bioassay
  20. Cardiac arterial pole alignment is sensitive to FGF8 signaling in the pharynx.
    Authors: Hutson MR, Zhang P, Stadt HA, Sato AK, Li YX, Burch J, Creazzo TL, Kirby ML
    Dev. Biol., 2006;295(2):486-97.
    Species: Chicken
    Sample Type: Whole Cells
    Application: Bioassay
  21. Expression of the short stature homeobox gene Shox is restricted by proximal and distal signals in chick limb buds and affects the length of skeletal elements.
    Authors: Tiecke E, Bangs F, Blaschke R, Farrell ER, Rappold G, Tickle C
    Dev. Biol., 2006;298(2):585-96.
    Species: Chicken
    Sample Type: In Vivo
    Application: In Vivo
  22. Canonical Wnt signaling is required for development of embryonic stem cell-derived mesoderm.
    Authors: Lindsley RC, Gill JG, Kyba M, Murphy TL, Murphy KM
    Development, 2006;133(19):3787-96.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  23. Embryonic stem cell-derived neuron models of Parkinson's disease exhibit delayed neuronal death.
    Authors: Yamashita H, Nakamura T, Takahashi T, Nagano Y, Hiji M, Hirabayashi T, Amano T, Yagi T, Sakai N, Kohriyama T, Matsumoto M
    J. Neurochem., 2006;98(1):45-56.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  24. Ventral midbrain glia express region-specific transcription factors and regulate dopaminergic neurogenesis through Wnt-5a secretion.
    Authors: Castelo-Branco G, Sousa KM, Bryja V, Pinto L, Wagner J, Arenas E
    Mol. Cell. Neurosci., 2005;31(2):251-62.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  25. Control of the segmentation process by graded MAPK/ERK activation in the chick embryo.
    Authors: Delfini MC, Dubrulle J, Malapert P, Chal J, Pourquie O
    Proc. Natl. Acad. Sci. U.S.A., 2005;102(32):11343-8.
    Species: Chicken
    Sample Type: In Vivo
    Application: In Vivo
  26. Depletion of Bmp2, Bmp4, Bmp7 and Spemann organizer signals induces massive brain formation in Xenopus embryos.
    Authors: Reversade B, Kuroda H, Lee H, Mays A, De Robertis EM
    Development, 2005;132(15):3381-92.
    Species: Xenopus
    Sample Type: In Vivo
    Application: In Vivo
  27. Derivation of midbrain dopamine neurons from human embryonic stem cells.
    Authors: Perrier AL, Tabar V, Barberi T, Rubio ME, Bruses J, Topf N, Harrison NL, Studer L
    Proc. Natl. Acad. Sci. U.S.A., 2004;101(34):12543-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  28. vhnf1 and Fgf signals synergize to specify rhombomere identity in the zebrafish hindbrain.
    Authors: Wiellette EL, Sive H
    Development, 2003;130(16):3821-9.
    Species: Zebrafish
    Sample Type: In Vivo
    Application: In Vivo
  29. Xenopus neurula left-right asymmetry is respeficied by microinjecting TGF-beta5 protein.
    Authors: Mogi K, Goto M, Ohno E, Azumi Y, Takeuchi S, Toyoizumi R
    Int. J. Dev. Biol., 2003;47(1):15-29.
    Species: Xenopus
    Sample Type: In Vivo
    Application: In Vivo
  30. Specification of dorsal telencephalic character by sequential Wnt and FGF signaling.
    Authors: Gunhaga L, Marklund M, Sjodal M, Hsieh JC, Jessell TM, Edlund T
    Nat. Neurosci., 2003;6(7):701-7.
    Species: Chicken
    Sample Type: Whole Tissue
    Application: Bioassay
  31. Fgf8 is required for pharyngeal arch and cardiovascular development in the mouse.
    Authors: Abu-Issa R, Smyth G, Smoak I, Yamamura K, Meyers EN
    Development, 2002;129(19):4613-25.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  32. Regulation of avian cardiogenesis by Fgf8 signaling.
    Authors: Alsan BH, Schultheiss TM
    Development, 2002;129(8):1935-43.
    Species: Chicken
    Sample Type: In Ovo
    Application: In Ovo
  33. FGF8 acts as a right determinant during establishment of the left-right axis in the rabbit.
    Authors: Fischer A, Viebahn C, Blum M
    Curr. Biol., 2002;12(21):1807-16.
    Species: Rabbit
    Sample Type: Whole Cells
    Application: Bioassay
Expand to show all 33 Citations

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