Retinoic acid

Tocris Bioscience | Catalog # 0695

Endogenous retinoic acid receptor agonist
Tocris Bioscience

Key Product Details

Description

Endogenous retinoic acid receptor agonist

Alternative Names

Tretinoin,ATRA
View all Retinoic Acid Receptor Agonists »

Product Description

Retinoic acid is an endogenous agonist for retinoic acid receptors (IC50 = 14 nM for RARα, RARβ and RARγ receptors). Retinoic acid specifies anterior-posterior patterning of neural progenitors. It promotes differentiation of mouse embryonic stem cells (ESCs) into adipocytes, neurons and glia in vitro. Retinoic acid also promotes differentiation of human PSCs into dopaminergic neurons, β cells and maturation of retinal organoids (see our protocol below). It is a proposed ligand of RORβ (Kd = 280 nM). Activates autophagy.

For more information about how Retinoic acid may be used, see our protocols: Generation of β cells from hPSCs, Cultivating Cerebral Organoids.

Retinoic acid synthesized to Ancillary Material Grade also available.

Scientific Data Images for Retinoic acid

iPSC differentiation with retinoic acid

IBJ6 human fibroblast induced pluripotent stem cells which have been differentiated into motor neurons.

Performed using a combination of LDN 193189 (100 nM) (Catalog # 6053,Tocris) and SB 431542 (Catalog # 1614, Tocris) followed by Retinoic acid and Purmorphamine (Catalog # 4551, Tocris) culture on Laminin for 18 days. Commitment to the motor neuron fate was assessed using the motor neuron marker anti-Human Islet-1 (red, Catalog # AF1837, R&D Systems) and the general neuronal marker, beta III tubulin (green, Catalog # MAB1195, R&D Systems). For visualization, the neurons were stained using Northernlights™ 557-conjugated Donkey anti-Goat Secondary Antibody (Catalog # NL001, R&D Systems) and Northernlights™ 493-conjugated Donkey anti-Mouse Secondary Antibody (Catalog # NL009, R&D Systems) and counterstained with DAPI (blue, Catalog # 5748, Tocris).

Product Specifications for Retinoic acid

Molecular Weight

300.44

Formula

C20H28O2

Storage

Store at -20°C

Purity

≥98% (HPLC)

Chemical Name

3,7-Dimethyl-9-(2,6,6-trimethyl-1-cyclohexen-1-yl)-2E,4E,6E,8E,-nonatetraenoic acid

CAS Number

302-79-4

PubChem ID

444795

InChI Key

SHGAZHPCJJPHSC-YCNIQYBTSA-N

SMILES

CC(/C=C/C1=C(C)CCCC1(C)C)=C\C=C\C(C)=C\C(O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 7.51 25
Ethanol 3 10

Preparing Stock Solutions for Retinoic acid

The following data is based on the product molecular weight 300.44.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.25 mM 13.31 mL 66.57 mL 133.14 mL
1.25 mM 2.66 mL 13.31 mL 26.63 mL
2.5 mM 1.33 mL 6.66 mL 13.31 mL
12.5 mM 0.27 mL 1.33 mL 2.66 mL

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 211 publications citing the usage of this product.

Product Documents for Retinoic acid

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Retinoic acid

For research use only

⚠ WARNING: This product can expose you to chemicals including All-trans retinoic acid, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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