LDN 193189 dihydrochloride

Catalog # Availability Size / Price Qty
LDN 193189 dihydrochloride | CAS No. 1435934-00-1 | BMP and Other Activin Receptor Inhibitors
1 Image
Description: Potent and selective ALK2 and ALK3 inhibitor; inhibits BMP4 signaling; promotes neural induction of hPSCs

Chemical Name: 4-[6-[4-(1-Piperazinyl)phenyl]pyrazolo[1,5-a]pyrimidin-3-yl]quinoline dihydrochloride

Purity: ≥98%

Product Details
Citations (6)
Supplemental Products
Reviews (3)

Biological Activity

Potent and selective ALK2 and ALK3 inhibitor (IC50 values are 5 and 30 nM, respectively); inhibits BMP4-mediated Smad1/5/8 activation. Exhibits >200-fold selectivity for BMP signaling over TGF-β signaling. Also exhibits selectivity over AMPK, PDGFR and MAPK signaling. Promotes neural induction of hPSCs in combination with SB 431542 (Cat.No. 1614). Also induces differentiation of hPSCs into nociceptive sensory neurons in combination with SB 431542 (Cat.No. 1614), SU 5402 (Cat.No.3300), CHIR 99021 (Cat.No. 4423) and DAPT (Cat.No. 2634).

Motor neurons differentiated from iPSCs with LDN 193189

iPS differentiation with LDN 193189

Image shows IBJ6 human fibroblast induced pluripotent stem cells which have been differentiated into motor neurons. This was performed using a combination of LDN 193189 (100 nM) and SB 431542 (Cat. No. 1614) followed by Retinoic acid (Cat. No. 0695) and Purmorphamine (Cat. No. 4551) culture on Laminin for 18 days. Commitment to the motor neuron fate was assessed using the motor neuron marker anti-Human Islet-1 (red, R&D Systems®, Cat. No. AF1837) and the general neuronal marker, beta III tubulin (green, R&D Systems®, Cat. No. MAB1195). For visualization, the neurons were stained using Northernlights™ 557-conjugated Donkey anti-Goat Secondary Antibody (R&D Systems®, Cat. No. NL001) and Northernlights™ 493-conjugated Donkey anti-Mouse Secondary Antibody (R&D Systems®, Cat. No. NL009) and counterstained with DAPI (blue, Cat. No. 5748).

Technical Data

Soluble to 50 mM in water and to 20 mM in DMSO
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Additional Information

Licensing Caveats:
Sold for research purposes under exclusive agreement from The Brigham and Women's Hospital Inc. US patents 8,507,501 and 9,045,484

Product Datasheets

Or select another batch:
Reconstitution Calculator
Molarity Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Molarity Calculator


*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Citations for LDN 193189 dihydrochloride

The citations listed below are publications that use Tocris products. Selected citations for LDN 193189 dihydrochloride include:

6 Citations: Showing 1 - 6


No product specific FAQs exist for this product, however you may

View all Small Molecule FAQs

Reviews for LDN 193189 dihydrochloride

Average Rating: 4 (Based on 3 Reviews)

5 Star
4 Star
3 Star
2 Star
1 Star

Have you used LDN 193189 dihydrochloride?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:

Efficient and effective inhibitor of BMP-like receptors
By Paul Humphreys on 12/11/2020
Application: Species: Human

To perform inhibition of BMP-like receptors during various interrogations. Performed blanket inhibition of BMP-like receptor kinase activity with 50nM (working) and selective inhibition of ALK3/BMPR1A with 5nM (working) leaving ALK6/BMPR1B active. Non-toxic at concentrations used.

PMID: 33084303

Differentiation to cortical neurons
By Julieta O'Flaherty on 12/11/2020
Application: Species: Human

I used the product for 10 days to differentiate ipscs into a neuroepithelium

BMP pathway inhibitor that works really well
By Anonymous on 12/09/2019
Application: Species: Human

I used it as a BMP pathway inhibitor in Breast cancer Cells. I used the concentration of 100nM and it works really well.

Tocris Bioscience is the leading supplier of novel and exclusive tools for life science research with over 30 years' experience in the industry. Tocris is a Bio-Techne brand.