Home / BMP and Other Activin Receptor Inhibitors / LDN 193189 dihydrochloride

LDN 193189 dihydrochloride

Catalog #: 6053
37 Citations 1 Review Datasheet / COA / SDS
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6053/50
LDN 193189 dihydrochloride | CAS No. 1435934-00-1 | BMP and Other Activin Receptor Inhibitors
2 Images
Description: Potent and selective ALK2 and ALK3 inhibitor; inhibits BMP4 signaling; promotes neural induction of hPSCs

Chemical Name: 4-[6-[4-(1-Piperazinyl)phenyl]pyrazolo[1,5-a]pyrimidin-3-yl]quinoline dihydrochloride

Purity: ≥98%

Product Details
Citations (37)
Supplemental Products
Reviews (1)
Biological Activity Scientific Data Technical Data Additional Information Background Product Datasheets Calculators

Biological Activity

LDN 193189 dihydrochloride is a potent and selective ALK2 and ALK3 inhibitor (IC50 values are 5 and 30 nM, respectively); inhibits BMP4-mediated Smad1/5/8 activation. Exhibits >200-fold selectivity for BMP signaling over TGF-β signaling. Also exhibits selectivity over AMPK, PDGFR and MAPK signaling. Promotes neural induction of hPSCs in combination with SB 431542 (Cat.No. 1614). Also induces differentiation of hPSCs into nociceptive sensory neurons in combination with SB 431542 (Cat.No. 1614), SU 5402 (Cat.No.3300), CHIR 99021 (Cat.No. 4423) and DAPT (Cat.No. 2634).

LDN 193189 synthesized to cGMP guidelines also available.

For more information about how LDN 193189 dihydrochloride may be used, see our protocols: Generation of β cells from hPSCs, Generating Midbrain Dopaminergic Neurons from hPSCs, Accelerated Induction of Cortical Neurons from hiPSCs.

Scientific Data

N/A Application of LDN 193189 in motor neurons differentiated from iPSCs View Larger

Application of LDN 193189 in motor neurons differentiated from iPSCs. Image shows IBJ6 human fibroblast induced pluripotent stem cells which have been differentiated into motor neurons. This was performed using a combination of LDN 193189 (100 nM) and SB 431542 (Catalog # 1614, Tocris) followed by Retinoic acid (Catalog # 0695, Tocris) and Purmorphamine (Catalog # 4551, Tocris) culture on Laminin for 18 days. Commitment to the motor neuron fate was assessed using the motor neuron marker anti-Human Islet-1 (red, R&D Systems, Catalog # AF1837) and the general neuronal marker, beta III tubulin (green, R&D Systems, Catalog # MAB1195). For visualization, the neurons were stained using Northernlights™ 557-conjugated Donkey anti-Goat Secondary Antibody (R&D Systems, Catalog # NL001) and Northernlights™ 493-conjugated Donkey anti-Mouse Secondary Antibody (R&D Systems, Catalog # NL009 NL009) and counterstained with DAPI (blue, Catalog # 5748, Tocris).

Technical Data

M.Wt:
479.4
Formula:
C25H22N6.2HCl
Solubility:
Soluble to 50 mM in water and to 10 mM in DMSO
Purity:
≥98%
Storage:
Store at -20°C
CAS No:
1435934-00-1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Additional Information

Licensing Caveats:
Sold for research purposes under exclusive agreement from The Brigham and Women's Hospital Inc. US patents 8,507,501 and 9,045,484

Product Datasheets

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Citations for LDN 193189 dihydrochloride

The citations listed below are publications that use Tocris products. Selected citations for LDN 193189 dihydrochloride include:

37 Citations: Showing 1 - 10

  1. A Robust Pipeline for the Multi-Stage Accelerated Differentiation of Functional 3D Cortical Organoids from Human Pluripotent Stem Cells.
    Authors: Mustafa Et al.
    Curr Protoc  2023;3:e641
  2. Expansion of ventral foregut is linked to changes in the enhancer landscape for organ-specific differentiation.
    Authors: Sara Et al.
    Nat Cell Biol  2023;25:481-492
  3. Generation and characterization of NGLY1 patient-derived midbrain organoids.
    Authors: Wei Et al.
    Front Cell Dev Biol  2023;11:1039182
  4. Multidimensional analysis and therapeutic development using patient iPSC-derived disease models of Wolfram syndrome.
    Authors: Mark J Et al.
    JCI Insight  2022;7
  5. Substantial somatic genomic variation and selection for BCOR mutations in human induced pluripotent stem cells.
    Authors: Richard Et al.
    Nat Genet  2022;54:1406-1416
  6. Classical Complement Pathway Inhibition in a Human-On-A-Chip" Model of Autoimmune Demyelinating Neuropathies."
    Authors: Timothy Et al.
    Adv Ther (Weinh)  2022;5
  7. An improved protocol for generation and characterization of human-induced pluripotent stem cell-derived retinal pigment epithelium cells.
    Authors: Surendran Et al.
    STAR Protoc  2022;3:101803
  8. Dynamic 3D Combinatorial Generation of hPSC-Derived Neuromesodermal Organoids With Diverse Regional and Cellular Identities.
    Authors: Mustafa Et al.
    Curr Protoc  2022;2:e568
  9. Derivation of nociceptive sensory neurons from hiPSCs with early patterning and temporally controlled NEUROG2 overexpression.
    Authors: Emmanouil Et al.
    Cell Rep Methods  2022;2:100341
  10. CRISPRi screens in human iPSC-derived astrocytes elucidate regulators of distinct inflammatory reactive states.
    Authors: Shinong Et al.
    Nat Neurosci  2022;25:1528-1542
  11. Wnt-activating human skin organoid model of atopic dermatitis induced by Staphylococcus aureus and its protective effects by Cutibacterium acnes.
    Authors: Kyung-Sun Et al.
    iScience  2022;25:105150
  12. Uncovering specificity of endogenous TAU aggregation in a human iPSC-neuron TAU seeding model.
    Authors: Jessica Et al.
    iScience  2022;25:103658
  13. Selective activation and down-regulation of Trk receptors by neurotrophins in human neurons co-expressing TrkB and TrkC.
    Authors: Jia Et al.
    J Neurochem  2022;161:463-477
  14. A pendulum of induction between the epiblast and extra-embryonic endoderm supports post-implantation progression.
    Authors: Jop Et al.
    Development  2022;149
  15. Distinct properties of Ca2+ efflux from brain, heart and liver mitochondria: The effects of Na+, Li+ and the mitochondrial Na+/Ca2+ exchange inhibitor CGP37157.
    Authors: Stefan Et al.
    Cell Calcium  2021;96:102382
  16. Publicly Available hiPSC Lines with Extreme Polygenic Risk Scores for Modeling Schizophrenia.
    Authors: Schahram Et al.
    Complex Psychiatry  2021;6:68-82
  17. Resolving cell state in iPSC-derived human neural samples with multiplexed fluorescence imaging.
    Authors: Beth A Et al.
    Commun Biol  2021;4:786
  18. RSPO2 inhibits BMP signaling to promote self-renewal in acute myeloid leukemia.
    Authors: Christof Et al.
    Cell Rep  2021;36:109559
  19. Commitment and oncogene-induced plasticity of human stem cell-derived pancreatic acinar and ductal organoids.
    Authors: Ling Et al.
    Cell Stem Cell  2021;28:1090-1104.e6
  20. Transplantable human motor networks as a neuron-directed strategy for spinal cord injury.
    Authors: Philip J Et al.
    iScience  2021;24:102827
  21. Human Pluripotent Stem Cells for High-Throughput Drug Screening and Characterization of Small Molecules.
    Authors: Ruili Et al.
    Methods Mol Biol  2021;2454:811-827
  22. In Vitro Generation of Posterior Motor Neurons from Human Pluripotent Stem Cells.
    Authors: Anestis Et al.
    Curr Protoc  2021;1:e244
  23. Robotic high-throughput biomanufacturing and functional differentiation of human pluripotent stem cells.
    Authors: Tristan Et al.
    Stem Cell Reports  2021;16:3076
  24. Altered temporal sequence of transcriptional regulators in the generation of human cerebellar granule cells.
    Authors: Thomas S Et al.
    Elife  2021;10
  25. Optogenetic Control of the BMP Signaling Pathway.
    Authors: Steven Et al.
    ACS Synth Biol  2020;9:3067-3078
  26. A Human Skeletal Muscle Atlas Identifies the Trajectories of Stem and Progenitor Cells across Development and from Human Pluripotent Stem Cells.
    Authors: Katja Et al.
    Cell Stem Cell  2020;27:158-176.e10
  27. Generation of PAX7 Reporter Cells to Investigate Skeletal Myogenesis from Human Pluripotent Stem Cells.
    Authors: April D Et al.
    STAR Protoc  2020;1:100158
  28. Aberrant interaction of FUS with the U1 snRNA provides a molecular mechanism of FUS induced amyotrophic lateral sclerosis.
    Authors: Frédéric H-T Et al.
    Nat Commun  2020;11:6341
  29. Cellular and molecular characterization of multiplex autism in human induced pluripotent stem cell-derived neurons.
    Authors: Bo Et al.
    Mol Autism  2020;10:51
  30. Axon-seq for in Depth Analysis of the RNA Content of Neuronal Processes.
    Authors: Eva Et al.
    Bio Protoc  2019;9:e3312
  31. Long-term functional maintenance of primary human hepatocytes in vitro.
    Authors: Xiang Et al.
    Science  2019;364:399
  32. Single-Cell RNA-Sequencing-Based CRISPRi Screening Resolves Molecular Drivers of Early Human Endoderm Development.
    Authors: Genga Et al.
    Cell Rep  2019;27:708
  33. Th17 lymphocytes induce neuronal cell death in a human iPSC-based model of Parkinson's disease.
    Authors: Sommer Et al.
    Cell Stem Cell  2018;23:123
  34. Mechanisms of neuroprotection against ischemic insult by stress-inducible phosphoprotein-1/prion protein complex.
    Authors: Beraldo Et al.
    J Neurochem  2018;145:68
  35. Specification of murine ground state pluripotent stem cells to regional neuronal populations.
    Authors: Alsanie
    Sci Rep  2017;7(1):16001
  36. Generation of Induced Pluripotent Stem Cells from Patients with COL3A1 Mutations and Differentiation to Smooth Muscle Cells for ECM-Surfaceome Analyses.
    Authors: Kenneth R Et al.
    Methods Mol Biol  2017;1722:261-302
  37. The doublesex-related Dmrta2 safeguards neural progenitor maintenance involving transcriptional regulation of Hes1.
    Authors: Young
    Proc Natl Acad Sci U S A.  2017;114(28):E5599

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VersaClone cDNA Plasmids

Human Activin RIA/ALK-2 (NP_001096) VersaClone cDNA

RDC1201

Human BMPR-IA/ALK-3 (NP_004320) VersaClone cDNA

RDC1176

Mouse BMPR-IA/ALK-3 (NP_033888) VersaClone cDNA

RDC1235

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BMP pathway inhibitor that works really well
By Anonymous on 12/09/2019
Species: Human

I used it as a BMP pathway inhibitor in Breast cancer Cells. I used the concentration of 100nM and it works really well.

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