LDN 193189 dihydrochloride

Tocris Bioscience | Catalog # 6053

Potent and selective ALK2 and ALK3 inhibitor; inhibits BMP4 signaling; promotes neural induction of hPSCs
Tocris Bioscience

Key Product Details

Description

Potent and selective ALK2 and ALK3 inhibitor; inhibits BMP4 signaling; promotes neural induction of hPSCs
View all BMP and Other Activin Receptor Inhibitors »

Product Description

LDN 193189 dihydrochloride is a potent and selective ALK2 and ALK3 inhibitor (IC50 values are 5 and 30 nM, respectively); inhibits BMP4-mediated Smad1/5/8 activation. Exhibits >200-fold selectivity for BMP signaling over TGF-β signaling. Also exhibits selectivity over AMPK, PDGFR and MAPK signaling. Promotes neural induction of hPSCs in combination with SB 431542 (Cat.No. 1614). Also induces differentiation of hPSCs into nociceptive sensory neurons in combination with SB 431542 (Cat.No. 1614), SU 5402 (Cat.No.3300), CHIR 99021 (Cat.No. 4423) and DAPT (Cat.No. 2634). Used in protocols to generate pancreatic beta cells, or midbrain dopaminergic neurons from hPSCs (see below).

LDN 193189 synthesized to cGMP guidelines and LDN 193189 in solution also available.

For more information about how LDN 193189 dihydrochloride may be used, see our protocols: Generation of β cells from hPSCs, Generating Midbrain Dopaminergic Neurons from hPSCs, Accelerated Induction of Cortical Neurons from hiPSCs.

Scientific Data Images for LDN 193189 dihydrochloride

Application of LDN 193189 in motor neurons differentiated from iPSCs

Application of LDN 193189 in motor neurons differentiated from iPSCs.

Image shows IBJ6 human fibroblast induced pluripotent stem cells which have been differentiated into motor neurons. This was performed using a combination of LDN 193189 (100 nM) and SB 431542 (Catalog # 1614, Tocris) followed by Retinoic acid (Catalog # 0695, Tocris) and Purmorphamine (Catalog # 4551, Tocris) culture on Laminin for 18 days. Commitment to the motor neuron fate was assessed using the motor neuron marker anti-Human Islet-1 (red, R&D Systems, Catalog # AF1837) and the general neuronal marker, beta III tubulin (green, R&D Systems, Catalog # MAB1195). For visualization, the neurons were stained using Northernlights™ 557-conjugated Donkey anti-Goat Secondary Antibody (R&D Systems, Catalog # NL001) and Northernlights™ 493-conjugated Donkey anti-Mouse Secondary Antibody (R&D Systems, Catalog # NL009 NL009) and counterstained with DAPI (blue, Catalog # 5748, Tocris).

Licensing Information

Sold for research purposes under exclusive agreement from The Brigham and Women's Hospital Inc. US patents 8,507,501 and 9,045,484

Product Specifications for LDN 193189 dihydrochloride

Molecular Weight

479.40

Formula

C25H22N6.2HCl

Storage

Store at -20°C

Purity

≥98% (HPLC)

Chemical Name

4-[6-[4-(1-Piperazinyl)phenyl]pyrazolo[1,5-a]pyrimidin-3-yl]quinoline dihydrochloride

CAS Number

1435934-00-1

PubChem ID

91900717

InChI Key

CMQXLLAILGGLRV-UHFFFAOYSA-N

SMILES

N1(C2=CC=C(C3=CN4N=CC(C5=CC=NC6=C5C=CC=C6)=C4N=C3)C=C2)CCNCC1.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 23.97 50
DMSO 4.79 10

Preparing Stock Solutions for LDN 193189 dihydrochloride

The following data is based on the product molecular weight 479.40.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.5 mM 4.17 mL 20.86 mL 41.72 mL
2.5 mM 0.83 mL 4.17 mL 8.34 mL
5 mM 0.42 mL 2.09 mL 4.17 mL
25 mM 0.08 mL 0.42 mL 0.83 mL

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 119 publications citing the usage of this product.

Product Documents for LDN 193189 dihydrochloride

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for LDN 193189 dihydrochloride

For research use only

Citations for LDN 193189 dihydrochloride

Customer Reviews for LDN 193189 dihydrochloride (3)

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  • Efficient and effective inhibitor of BMP-like receptors
    Name: Paul Humphreys
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: TC28a2
    Verified Customer | Posted 12/11/2020
    Be wary of storage as activity will degrade over time.
    To perform inhibition of BMP-like receptors during various interrogations. Performed blanket inhibition of BMP-like receptor kinase activity with 50nM (working) and selective inhibition of ALK3/BMPR1A with 5nM (working) leaving ALK6/BMPR1B active. Non-toxic at concentrations used.
    LDN 193189 dihydrochloride 6053
  • Differentiation to cortical neurons
    Name: Julieta O'Flaherty
    Species: Human
    Cell Line/Tissue: Induced pluripotent stem cells
    Verified Customer | Posted 12/11/2020
    I used the product for 10 days to differentiate ipscs into a neuroepithelium
    LDN 193189 dihydrochloride 6053
    Bio-Techne Response
    Thank you for reviewing our product. We are sorry to hear that this compound did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.
  • BMP pathway inhibitor that works really well
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: Breast Cancer
    Verified Customer | Posted 12/09/2019
    I used it as a BMP pathway inhibitor in Breast cancer Cells. I used the concentration of 100nM and it works really well.

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