LDN 193189 dihydrochloride

  (2 citations)   
Catalog Number:6053
Chemical Name:4-[6-[4-(1-Piperazinyl)phenyl]pyrazolo[1,5-a]pyrimidin-3-yl]quinoline dihydrochloride
Product Details
Citations (2)
Reviews
Biological Activity
Potent and selective ALK2 and ALK3 inhibitor (IC50 values are 5 and 30 nM, respectively); inhibits BMP4-mediated Smad1/5/8 activation. Exhibits >200-fold selectivity for BMP signaling over TGF-β signaling. Also exhibits selectivity over AMPK, PDGFR and MAPK signaling. Promotes neural induction of hPSCs in combination with SB 431542 (Cat.No. 1614). Also induces differentiation of hPSCs into nociceptive sensory neurons in combination with SB 431542 (Cat.No. 1614), SU 5402 (Cat.No.3300), CHIR 99021 (Cat.No. 4423) and DAPT (Cat.No. 2634).
iPS differentiation with LDN 193189
iPS differentiation with LDN 193189

Image shows IBJ6 human fibroblast induced pluripotent stem cells which have been differentiated into motor neurons. This was performed using a combination of LDN 193189 (100 nM) and SB 431542 (Cat. No. 1614) followed by Retinoic acid (Cat. No. 0695) and Purmorphamine (Cat. No. 4551) culture on Laminin for 18 days. Commitment to the motor neuron fate was accessed using the motor neuron marker anti-Human Islet-1 (red, R&D Systems®, Cat. No. AF1837) and the general neuronal marker, beta III tubulin (green, R&D Systems®, Cat. No. MAB1195). For visualization, the neurons were stained using Northernlights™ 557-conjugated Donkey anti-Goat Secondary Antibody (R&D Systems®, Cat. No. NL001) and Northernlights™ 493-conjugated Donkey anti-Mouse Secondary Antibody (R&D Systems®, Cat. No. NL009).

Technical Data
  • M.Wt:
    479.4
  • Formula:
    C25H22N6.2HCl
  • Solubility:
    Soluble to 50 mM in water and to 20 mM in DMSO
  • Purity:
    >98%
  • Storage:
    Store at -20°C
  • CAS No:
    1435934-00-1
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis. All Tocris products are intended for laboratory research use only.
Additional Information
Licensing Caveats:
Sold for research purposes under exclusive agreement from The Brigham and Women's Hospital Inc. US patents 8,507,501 and 9,045,484
Citations:

The citations listed below are publications that use Tocris products. Selected citations for LDN 193189 dihydrochloride include:

2 Citations: Showing 1 - 2
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  1. Specification of murine ground state pluripotent stem cells to regional neuronal populations.
    Authors: Alsanie
    Sci Rep  2017;7(1):16001
  2. The doublesex-related Dmrta2 safeguards neural progenitor maintenance involving transcriptional regulation of Hes1.
    Authors: Young
    Proc Natl Acad Sci U S A.  2017;114(28):E5599

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