Human Islet-1 Antibody

Catalog # Availability Size / Price Qty
AF1837
AF1837-SP
Human Islet-1 Antibody in Western Blot
3 Images
Product Details
Citations (11)
FAQs
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Human Islet-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Islet-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Islet-2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Islet-1
Met4-Ala349
Accession # P61371
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Islet‑1 by Western Blot. View Larger

Detection of Human Islet‑1 by Western Blot. Western blot shows lysates of JOY6 human induced pluripotent stem cells undifferentiated and differentiatied into motor neurons. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Islet-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1837) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Islet-1 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Islet‑1 in iPS2 Human Stem Cells. View Larger

Islet‑1 in iPS2 Human Stem Cells. Islet-1 was detected in immersion fixed iPS2 human induced pluripotent stem cells differentiated to endocrine progenitor cells using Goat Anti-Human Islet-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1837) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI(blue). Specific staining was localized to the nucleus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western Detection of Human Islet‑1 by Simple Western<sup>TM</sup>. View Larger

Detection of Human Islet‑1 by Simple WesternTM. Simple Western lane view shows lysates of JOY6 human induced pluripotent stem cells undifferentiated and differentiated into motor neurons, loaded at 0.2 mg/mL. A specific band was detected for Islet-1 at approximately 53 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Islet-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1837) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Islet-1

Islet-1 is a member of a family of homeodomain containing transcription factors. It is expressed in all islet cells in the pancreas and is an early marker for motor neuron differentiation.

Entrez Gene IDs
3670 (Human)
Alternate Names
insulin gene enhancer protein ISL-1; ISL LIM homeobox 1; ISL1 transcription factor, LIM/homeodomain; ISL1 transcription factor, LIM/homeodomain, (islet-1); ISL1; Isl-1; Islet1; Islet-1

Product Datasheets

Citations for Human Islet-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. The cofilin/Limk1 pathway controls the growth rate of both developing and regenerating motor axons
    Authors: ME Frendo, A da Silva, KD Phan, S Riche, SJ Butler
    J. Neurosci., 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Gene activation of SMN by selective disruption of lncRNA-mediated recruitment of PRC2 for the treatment of spinal muscular atrophy
    Authors: CJ Woo, VK Maier, R Davey, J Brennan, G Li, J Brothers, B Schwartz, S Gordo, A Kasper, TR Okamoto, HE Johansson, B Mandefro, D Sareen, P Bialek, BN Chau, B Bhat, D Bullough, J Barsoum
    Proc. Natl. Acad. Sci. U.S.A, 2017;114(8):E1509-E1518.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. Foxo1 is a downstream effector of Isl1 in direct pathway striatal projection neuron development within the embryonic mouse telencephalon
    Authors: RR Waclaw, LA Ehrman, P Merchan-Sa, V Kohli, D Nardini, K Campbell
    Mol. Cell. Neurosci, 2017;80(0):44-51.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Cell freezing protocol suitable for ATAC-Seq on motor neurons derived from human induced pluripotent stem cells
    Sci Rep, 2016;6(0):25474.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC-P
  5. Foxp1-mediated programming of limb-innervating motor neurons from mouse and human embryonic stem cells.
    Authors: Adams, Katrina, Rousso, David L, Umbach, Joy A, Novitch, Bennett
    Nat Commun, 2015;6(0):6778.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: IHC
  6. Expression of the immunoglobulin superfamily cell adhesion molecules in the developing spinal cord and dorsal root ganglion.
    Authors: Gu, Zirong, Imai, Fumiyasu, Kim, In Jung, Fujita, Hiroko, Katayama, Kei ichi, Mori, Kensaku, Yoshihara, Yoshihir, Yoshida, Yutaka
    PLoS ONE, 2015;10(3):e0121550.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Isl1 and Pou4f2 form a complex to regulate target genes in developing retinal ganglion cells.
    Authors: Li, Renzhong, Wu, Fuguo, Ruonala, Raili, Sapkota, Darshan, Hu, Zihua, Mu, Xiuqian
    PLoS ONE, 2014;9(3):e92105.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ChIP
  8. LIM-homeodomain transcription factor Isl-1 mediates the effect of leptin on insulin secretion in mice.
    Authors: Chen J, Fu R, Cui Y, Li Y, Pan J, Liu J, Luo H, Yin J, Li D, Cui S
    J Biol Chem, 2013;288(17):12395-405.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Inhibitory Smads differentially regulate cell fate specification and axon dynamics in the dorsal spinal cord.
    Authors: Hazen VM, Phan KD, Hudiburgh S, Butler SJ
    Dev. Biol., 2011;356(2):566-75.
    Species: Chicken
    Sample Types: Whole Tissue
    Applications: IHC
  10. Cardiogenol C can induce Mouse Hair Bulge Progenitor Cells to Transdifferentiate into Cardiomyocyte-like Cells.
    Authors: Yau WW, Tang MK, Chen E
    Proteome Sci, 2011;9(1):3.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  11. Directed differentiation of human-induced pluripotent stem cells generates active motor neurons.
    Authors: Karumbayaram S, Novitch BG, Patterson M, Umbach JA, Richter L, Lindgren A, Conway AE, Clark AT, Goldman SA, Plath K, Wiedau-Pazos M, Kornblum HI, Lowry WE
    Stem Cells, 2009;27(4):806-11.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC

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