Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Avian - Chicken, Chicken, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry, Simple Western

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Islet-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Islet-1
Met4-Ala349
Accession # P61371

Specificity

Detects human Islet-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Islet-2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Islet‑1 Antibody

Detection of Human Islet-1 antibody by Western Blot.

Detection of Human Islet‑1 by Western Blot.

Western blot shows lysates of JOY6 human induced pluripotent stem cells undifferentiated and differentiatied into motor neurons. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Islet-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1837) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Islet-1 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Islet-1 antibody in iPS2 Human Stem Cells by Immunocytochemistry (ICC).

Islet‑1 in iPS2 Human Stem Cells.

Islet-1 was detected in immersion fixed iPS2 human induced pluripotent stem cells differentiated to endocrine progenitor cells using Goat Anti-Human Islet-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1837) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI(blue). Specific staining was localized to the nucleus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Islet‑1 in Human Pancreas.

Islet‑1 was detected in immersion fixed paraffin-embedded sections of human pancreas using Goat Anti-Human Islet‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1837) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in islet cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Human Islet-1 antibody by Simple WesternTM.

Detection of Human Islet‑1 by Simple WesternTM.

Simple Western lane view shows lysates of JOY6 human induced pluripotent stem cells undifferentiated and differentiated into motor neurons, loaded at 0.2 mg/mL. A specific band was detected for Islet-1 at approximately 53 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Islet-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1837) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Mouse Islet-1 by Immunohistochemistry

Detection of Mouse Islet-1 by Immunohistochemistry

Efficiency of Isl1 ablation in stomachs of Isl1MCM/Del mutant mouse stomachs at E18.5. (A) Tamoxifen-inducible Cre recombinase excised DNA sequences flanked by two loxP sites. (B)Isl1 RNA levels were ablated in Isl1MCM/Del mutant stomachs as seen by semi-quantitative PCR. Isl1F/+mice showed a 592 base pair product whereas Isl1MCM/Del mice generated a 303 base pair product. (C) Isl1 was significantly down-regulated at the protein levels in Isl1MCM/Del mutant stomachs as shown by western blot. Expression of embryos at E11.5 was used as positive control. (D) Isl1 protein expression in Isl1F/+and Isl1MCM/Del embryonic pylorus. Isl1 expression was significantly reduced in Isl1MCM/Del embryonic stomachs, as seen by immunofluorescence. Images in Isl1F/+and Isl1MCM/Del were processed on the same slide and photographed at the same exposure. Enlarged images of the boxed areas are shown on the right side of the merged pictures. Yellow arrowheads show representative Isl1-positive cells, and white arrowheads show representative Isl1-negative cells. Yellow dotted lines mark the epithelial basement membrane. Scale bars: 50 μm. Image collected and cropped by CiteAb from the following publication (https://bmcbiol.biomedcentral.com/articles/10.1186/1741-7007-12-25), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Islet‑1 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed iPS2 human induced pluripotent stem cells differentiated to endocrine progenitor cells

Immunohistochemistry

1-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreas

Simple Western

10 µg/mL
Sample: JOY6 human induced pluripotent stem cells undifferentiated and differentiatied into Motor Neurons

Western Blot

1 µg/mL
Sample: JOY6 human induced pluripotent stem cells undifferentiated and differentiatied into Motor Neurons

Reviewed Applications

Read 1 review rated 5 using AF1837 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Islet-1

Islet-1 is a member of a family of homeodomain containing transcription factors. It is expressed in all islet cells in the pancreas and is an early marker for motor neuron differentiation.

Alternate Names

ISL1, Islet1

Entrez Gene IDs

3670 (Human)

Gene Symbol

ISL1

UniProt

P61371

Additional Islet-1 Products

Product Documents for Human Islet‑1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Islet‑1 Antibody

For research use only

Citations for Human Islet‑1 Antibody

Customer Reviews for Human Islet‑1 Antibody (1)

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  • Human Islet-1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: E10.5 mouse embryo fixed in 4% PFA
    Species: Mouse
    Verified Customer | Posted 12/02/2020
    Excellent antibody. Very reliable. Highly recommend on embryonic muse tissues. Have stained on both E9.5 and E10.5 mouse sections. Concentration used - 10ug/mL.
    Human Islet‑1 Antibody AF1837

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