Human Islet-1 Antibody

(9 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Islet-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Islet-2 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Islet-1
    Met4-Ala349
    Accession # P61371
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Islet‑1 by Western Blot. Western blot shows lysates of JOY6 human induced pluripotent stem cells undifferentiated and differentiatied into motor neurons. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Islet‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1837) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Islet‑1 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
Islet‑1 in iPS2 Human Stem Cells. Islet‑1 was detected in immersion fixed iPS2 human induced pluripotent stem cells differentiated to endocrine progenitor cells using Goat Anti-Human Islet‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1837) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI(blue). Specific staining was localized to the nucleus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human Islet‑1 by Simple WesternTM. Simple Western lane view shows lysates of JOY6 human induced pluripotent stem cells undifferentiated and differentiated into motor neurons, loaded at 0.2 mg/mL. A specific band was detected for Islet‑1 at approximately 53 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Islet‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1837) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Islet-1
Islet-1 is a member of a family of homeodomain containing transcription factors. It is expressed in all islet cells in the pancreas and is an early marker for motor neuron differentiation.
  • Entrez Gene IDs:
    3670 (Human)
  • Alternate Names:
    insulin gene enhancer protein ISL-1; ISL LIM homeobox 1; ISL1 transcription factor, LIM/homeodomain; ISL1 transcription factor, LIM/homeodomain, (islet-1); ISL1; Isl-1; Islet1; Islet-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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Species
Applications
Sample Type
  1. Gene activation of SMN by selective disruption of lncRNA-mediated recruitment of PRC2 for the treatment of spinal muscular atrophy
    Authors: CJ Woo, VK Maier, R Davey, J Brennan, G Li, J Brothers, B Schwartz, S Gordo, A Kasper, TR Okamoto, HE Johansson, B Mandefro, D Sareen, P Bialek, BN Chau, B Bhat, D Bullough, J Barsoum
    Proc. Natl. Acad. Sci. U.S.A, 2017;114(8):E1509-E1518.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  2. Cell freezing protocol suitable for ATAC-Seq on motor neurons derived from human induced pluripotent stem cells
    Sci Rep, 2016;6(0):25474.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC - Paraffin embedded
  3. Expression of the immunoglobulin superfamily cell adhesion molecules in the developing spinal cord and dorsal root ganglion.
    Authors: Gu, Zirong, Imai, Fumiyasu, Kim, In Jung, Fujita, Hiroko, Katayama, Kei ichi, Mori, Kensaku, Yoshihara, Yoshihir, Yoshida, Yutaka
    PLoS ONE, 2015;10(3):e0121550.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Not Specified
  4. Foxp1-mediated programming of limb-innervating motor neurons from mouse and human embryonic stem cells.
    Authors: Adams, Katrina, Rousso, David L, Umbach, Joy A, Novitch, Bennett
    Nat Commun, 2015;6(0):6778.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: IHC Not Specified
  5. Isl1 and Pou4f2 form a complex to regulate target genes in developing retinal ganglion cells.
    Authors: Li, Renzhong, Wu, Fuguo, Ruonala, Raili, Sapkota, Darshan, Hu, Zihua, Mu, Xiuqian
    PLoS ONE, 2014;9(3):e92105.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: ChIP
  6. LIM-homeodomain transcription factor Isl-1 mediates the effect of leptin on insulin secretion in mice.
    Authors: Chen J, Fu R, Cui Y, Li Y, Pan J, Liu J, Luo H, Yin J, Li D, Cui S
    J Biol Chem, 2013;288(17):12395-405.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  7. Inhibitory Smads differentially regulate cell fate specification and axon dynamics in the dorsal spinal cord.
    Authors: Hazen VM, Phan KD, Hudiburgh S, Butler SJ
    Dev. Biol., 2011;356(2):566-75.
    Species: Chicken
    Sample Type: Whole Tissue
    Application: IHC
  8. Cardiogenol C can induce Mouse Hair Bulge Progenitor Cells to Transdifferentiate into Cardiomyocyte-like Cells.
    Authors: Yau WW, Tang MK, Chen E
    Proteome Sci, 2011;9(1):3.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  9. Directed differentiation of human-induced pluripotent stem cells generates active motor neurons.
    Authors: Karumbayaram S, Novitch BG, Patterson M, Umbach JA, Richter L, Lindgren A, Conway AE, Clark AT, Goldman SA, Plath K, Wiedau-Pazos M, Kornblum HI, Lowry WE
    Stem Cells, 2009;27(4):806-11.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
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