Human Islet-1 Antibody

Catalog # Availability Size / Price Qty
AF1837
AF1837-SP
Detection of Human Islet‑1 by Western Blot.
5 Images
Product Details
Citations (16)
FAQs
Supplemental Products
Reviews (1)

Human Islet-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Islet-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Islet-2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Islet-1
Met4-Ala349
Accession # P61371
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunohistochemistry
1-15 µg/mL
Immersion fixed paraffin-embedded sections of human pancreas
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Islet-1 antibody by Western Blot. View Larger

Detection of Human Islet‑1 by Western Blot. Western blot shows lysates of JOY6 human induced pluripotent stem cells undifferentiated and differentiatied into motor neurons. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Islet-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1837) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Islet-1 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Islet-1 antibody in iPS2 Human Stem Cells by Immunocytochemistry (ICC). View Larger

Islet‑1 in iPS2 Human Stem Cells. Islet-1 was detected in immersion fixed iPS2 human induced pluripotent stem cells differentiated to endocrine progenitor cells using Goat Anti-Human Islet-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1837) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI(blue). Specific staining was localized to the nucleus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry View Larger

Islet‑1 in Human Pancreas. Islet‑1 was detected in immersion fixed paraffin-embedded sections of human pancreas using Goat Anti-Human Islet‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1837) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in islet cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western Detection of Human Islet-1 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human Islet‑1 by Simple WesternTM. Simple Western lane view shows lysates of JOY6 human induced pluripotent stem cells undifferentiated and differentiated into motor neurons, loaded at 0.2 mg/mL. A specific band was detected for Islet-1 at approximately 53 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Islet-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1837) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunohistochemistry Detection of Mouse Islet-1 by Immunohistochemistry View Larger

Detection of Mouse Islet-1 by Immunohistochemistry Efficiency of Isl1 ablation in stomachs of Isl1MCM/Del mutant mouse stomachs at E18.5. (A) Tamoxifen-inducible Cre recombinase excised DNA sequences flanked by two loxP sites. (B)Isl1 RNA levels were ablated in Isl1MCM/Del mutant stomachs as seen by semi-quantitative PCR. Isl1F/+mice showed a 592 base pair product whereas Isl1MCM/Del mice generated a 303 base pair product. (C) Isl1 was significantly down-regulated at the protein levels in Isl1MCM/Del mutant stomachs as shown by western blot. Expression of embryos at E11.5 was used as positive control. (D) Isl1 protein expression in Isl1F/+and Isl1MCM/Del embryonic pylorus. Isl1 expression was significantly reduced in Isl1MCM/Del embryonic stomachs, as seen by immunofluorescence. Images in Isl1F/+and Isl1MCM/Del were processed on the same slide and photographed at the same exposure. Enlarged images of the boxed areas are shown on the right side of the merged pictures. Yellow arrowheads show representative Isl1-positive cells, and white arrowheads show representative Isl1-negative cells. Yellow dotted lines mark the epithelial basement membrane. Scale bars: 50 μm. Image collected and cropped by CiteAb from the following publication (https://bmcbiol.biomedcentral.com/articles/10.1186/1741-7007-12-25), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Islet-1

Islet-1 is a member of a family of homeodomain containing transcription factors. It is expressed in all islet cells in the pancreas and is an early marker for motor neuron differentiation.

Entrez Gene IDs
3670 (Human)
Alternate Names
insulin gene enhancer protein ISL-1; ISL LIM homeobox 1; ISL1 transcription factor, LIM/homeodomain; ISL1 transcription factor, LIM/homeodomain, (islet-1); ISL1; Isl-1; Islet1; Islet-1

Product Datasheets

You must select a language.

x

Citations for Human Islet-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. In�vitro atlas of dorsal spinal interneurons reveals Wnt signaling as a critical regulator of progenitor expansion
    Authors: S Gupta, R Kawaguchi, E Heinrichs, S Gallardo, S Castellano, I Mandric, BG Novitch, SJ Butler
    Oncogene, 2022-07-19;40(3):111119.
    Species: Human
    Sample Types: Embryoid Bodies
    Applications: IHC
  2. Delamination of trophoblast-like syncytia from the amniotic ectodermal analogue in human primed embryonic stem cell-based differentiation model
    Authors: M Ohgushi, N Taniyama, A Vandenbon, M Eiraku
    Cell Reports, 2022-06-21;39(12):110973.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  3. Deciphering the spatial-temporal transcriptional landscape of human hypothalamus development
    Authors: X Zhou, Y Lu, F Zhao, J Dong, W Ma, S Zhong, M Wang, B Wang, Y Zhao, Y Shi, Q Ma, T Lu, J Zhang, X Wang, Q Wu
    Cell Stem Cell, 2021-12-07;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  4. Generation of Functional Brown Adipocytes from Human Pluripotent Stem Cells via Progression through a Paraxial Mesoderm State
    Authors: L Zhang, J Avery, A Yin, AM Singh, TS Cliff, H Yin, S Dalton
    Cell Stem Cell, 2020-08-11;0(0):.
    Species: Human
    Sample Types: Organoid
    Applications: IHC
  5. The cofilin/Limk1 pathway controls the growth rate of both developing and regenerating motor axons
    Authors: ME Frendo, A da Silva, KD Phan, S Riche, SJ Butler
    J. Neurosci., 2019-10-02;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Foxo1 is a downstream effector of Isl1 in direct pathway striatal projection neuron development within the embryonic mouse telencephalon
    Authors: RR Waclaw, LA Ehrman, P Merchan-Sa, V Kohli, D Nardini, K Campbell
    Mol. Cell. Neurosci, 2017-02-14;80(0):44-51.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Gene activation of SMN by selective disruption of lncRNA-mediated recruitment of PRC2 for the treatment of spinal muscular atrophy
    Authors: CJ Woo, VK Maier, R Davey, J Brennan, G Li, J Brothers, B Schwartz, S Gordo, A Kasper, TR Okamoto, HE Johansson, B Mandefro, D Sareen, P Bialek, BN Chau, B Bhat, D Bullough, J Barsoum
    Proc. Natl. Acad. Sci. U.S.A, 2017-02-13;114(8):E1509-E1518.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  8. Cell freezing protocol suitable for ATAC-Seq on motor neurons derived from human induced pluripotent stem cells
    Sci Rep, 2016-05-05;6(0):25474.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC-P
  9. Foxp1-mediated programming of limb-innervating motor neurons from mouse and human embryonic stem cells.
    Authors: Adams, Katrina, Rousso, David L, Umbach, Joy A, Novitch, Bennett
    Nat Commun, 2015-04-14;6(0):6778.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: IHC
  10. Expression of the immunoglobulin superfamily cell adhesion molecules in the developing spinal cord and dorsal root ganglion.
    Authors: Gu, Zirong, Imai, Fumiyasu, Kim, In Jung, Fujita, Hiroko, Katayama, Kei ichi, Mori, Kensaku, Yoshihara, Yoshihir, Yoshida, Yutaka
    PLoS ONE, 2015-03-31;10(3):e0121550.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Isl1 and Pou4f2 form a complex to regulate target genes in developing retinal ganglion cells.
    Authors: Li, Renzhong, Wu, Fuguo, Ruonala, Raili, Sapkota, Darshan, Hu, Zihua, Mu, Xiuqian
    PLoS ONE, 2014-03-18;9(3):e92105.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ChIP
  12. LIM-homeodomain transcription factor Isl-1 mediates the effect of leptin on insulin secretion in mice.
    Authors: Chen J, Fu R, Cui Y, Li Y, Pan J, Liu J, Luo H, Yin J, Li D, Cui S
    J Biol Chem, 2013-03-15;288(17):12395-405.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  13. Inhibitory Smads differentially regulate cell fate specification and axon dynamics in the dorsal spinal cord.
    Authors: Hazen VM, Phan KD, Hudiburgh S, Butler SJ
    Dev. Biol., 2011-06-22;356(2):566-75.
    Species: Chicken
    Sample Types: Whole Tissue
    Applications: IHC
  14. Cardiogenol C can induce Mouse Hair Bulge Progenitor Cells to Transdifferentiate into Cardiomyocyte-like Cells.
    Authors: Yau WW, Tang MK, Chen E
    Proteome Sci, 2011-01-19;9(1):3.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  15. Directed differentiation of human-induced pluripotent stem cells generates active motor neurons.
    Authors: Karumbayaram S, Novitch BG, Patterson M, Umbach JA, Richter L, Lindgren A, Conway AE, Clark AT, Goldman SA, Plath K, Wiedau-Pazos M, Kornblum HI, Lowry WE
    Stem Cells, 2009-04-01;27(4):806-11.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  16. IGF-I increases the recruitment of GLUT4 and GLUT3 glucose transporters on cell surface in hyperthyroidism.
    Authors: Dimitriadis G, Maratou E, Boutati E
    Eur. J. Endocrinol., 2008-03-01;158(3):361-6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody

WB,ELISA

Reviews for Human Islet-1 Antibody

Average Rating: 5 (Based on 1 Review)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human Islet-1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human Islet-1 Antibody
By Anonymous on 12/02/2020
Application: Immunocytochemistry/Immunofluorescence Sample Tested: E10.5 mouse embryo fixed in 4% PFA Species: Mouse

Excellent antibody. Very reliable. Highly recommend on embryonic muse tissues.
Have stained on both E9.5 and E10.5 mouse sections. Concentration used - 10ug/mL.