SARS-CoV-2 NSP7 Antibody

Catalog # Availability Size / Price Qty
MAB12505-100
MAB12505-SP
Detection of SARS-CoV-2 Nsp7 by Western Blot.
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SARS-CoV-2 NSP7 Antibody Summary

Species Reactivity
SARS-CoV-2
Specificity
Detects SARS-CoV-2 NSP7 in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 1045511
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived SARS-CoV-2 NSP7 protein
Ser1-Gln83
Accession # YP_009725303.1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

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Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
Recombinant SARS-CoV-2 Nsp7 protein

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot View Larger

Detection of SARS-CoV-2 Nsp7 by Western Blot. Western blot shows recombinant SARS-CoV-2 Nsp7 protein. PVDF membrane was probed with 2 µg/mL of Mouse Anti-SARS-CoV-2 NSP7 Monoclonal Antibody (Catalog # MAB12505) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Nsp7 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NSP7

Non-structural protein 7 (NSP7) is one of several functional proteins released by ORF1a-encoded protease cleavage of the pp1a and pp1ab replicase polyproteins expressed from the coronavirus (CoV) genome (1). The NSPs are involved in the replication and transcription of the viral RNA and not incorporated within the virion particles. Coronaviruses include various highly pathogenic strains such as SARS-CoV, MERS-CoV and SARS-CoV-2 that have had significant impact on humans in addition to strains that have negatively impacted livestock. NSP7 is a small 83 amino acid protein that is highly conserved across coronaviruses (1). The NSP7 monomers are composed of a central core N-terminal tri-helical bundle with an additional short C-terminal helix (2). The monomeric units associate to form a large hexadecameric structure with NSP8 (2) where layers of NSP7 fill the spaces in between NSP8 units. The supercomplexes are stacked to form a channel with electrostatic properties that could allow RNA to pass through the channel to facilitate efficient replication and transcription. The NSP7/NSP8 supercomplex was thereby proposed to function as a primase for the viral RNA-dependent RNA polymerase (RdRp), NSP12 (3). In SARS-CoV-2, the RdRp has been shown to have little activity without NSP8/7 as cofactors (4) making NSP7/NSP8 critical for viral polymerase activity. NSP7 has also been shown to interact with several other viral NSP proteins, including NSP5, NSP9, and NSP13 (5) as well as multiple host cell proteins involved in membrane trafficking, signaling, and electron transport including potential drug targets such as COMT and PTGES (6). 

References
  1. Snijder, E.J. et al. (2016) Adv. Virus Res. 96:59.
  2. Zhai, Y. et al. (2005) Nat. Struct. Mol. Bio. 12:980.
  3. Subissi, L. et al. (2014) Antiviral Res. 101:122.
  4. Yin, W. et al. (2020) Science 368:1499.
  5. von Brunn, A. et al. (2007) PLoS One 2:e459.
  6. Gordon, D.E. et al. (2020) Nature 583:459.
Long Name
Non-Structural Protein 7
Alternate Names
NSP7

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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