Key Product Details

Species Reactivity

SARS-CoV-2

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1044215
View all NSP9 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived SARS-CoV-2 NSP9
Asn1-Gln113
Accession # YP_009725305.1

Specificity

Detects SARS-CoV-2 NSP9 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for SARS-CoV-2 NSP9 Antibody

Detection of SARS-CoV-2 NSP9 by Western Blot.

Western blot shows recombinant SARS-CoV-2 NSP9. PVDF membrane was probed with 2 µg/mL of Mouse Anti-SARS-CoV-2 NSP9 Monoclonal Antibody (Catalog # MAB11063) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for NSP9 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Applications for SARS-CoV-2 NSP9 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant SARS-CoV-2 NSP9

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NSP9

Non-structural protein 9 (NSP9) is one of several functional proteins released by ORF1a-encoded protease cleavage of the pp1a and pp1ab replicase polyproteins expressed from the coronavirus (CoV) genome (1). The NSPs are involved in the replication and transcription of the viral RNA and not incorporated within the virion particles. Coronaviruses include various highly pathogenic strains such as SARS-CoV, MERS-CoV and SARS-CoV2 that have had significant impact on humans as well as strains that have negatively impacted livestock. NSP9 is a small 113 amino acid protein that forms a biologically active homodimer where each monomer consists of a beta barrel and C-terminal helical domain motif that promotes obligate dimerization (2,3). NSP9 is capable of binding nucleic acids in a nonsequence-specific manner with a preference of a single stranded RNA (4,5) although disruption of the dimeric interface appears to impact RNA binding (6). The NSP9 sequence is conserved across coronaviruses (3). NSP9 was shown to interact with other viral NSP proteins including NSP7, NSP8, and NSP12 (5,7,8). In addition, NSP9 has been shown to bind host cell proteins including DEAD-box RNA helicase 5 (DDX5), the ubiquitin E3 ubiquitin ligase MIB1, and elongation factor eIF4H in SARS-CoV2 and related viruses (9,10). The interactions of NSP9 with these host cell proteins promote viral replication (9,10) supporting the conclusion that NSP9 is important for virulence (2,3).

References

  1. Snijder, E.J. et al. (2016) Adv. Virus Res. 96:59.
  2. Miknis, Z.J. et al. (2009) J. Virol. 83:3007. 
  3. Littler, D.R. et al. (2020) iScience 23:101258
  4. Egloff, M.P. et al. (2004) Proc. Nat. Acad. Sci. U.S.A. 101:3792.
  5. Sutton, G. et. al. (2004) Structure 12:341.
  6. Hu, T. et al. (2017) Protein Sci. 26:1037.
  7. von Brunn, A. et al. (2007) PLoS One 2:e459.
  8. Chen, J. et al. (2017) Front. Microbiol. 8:853.
  9. Zhao, S. et al. (2015) Virus Res. 195:217.
  10. Gordon, D.E. et al. (2020) Nature 583:459.

Long Name

Non-Structural Protein 9

Alternate Names

Non-structural protein 9, ORF1a polyprotein, ORF1ab polyprotein, p12

UniProt

YP_009725305.1

Additional NSP9 Products

Product Documents for SARS-CoV-2 NSP9 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for SARS-CoV-2 NSP9 Antibody

For research use only

Related Research Areas

Infectious Diseases

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