SARS-CoV-2 Spike S2 Subunit Antibody

Catalog # Availability Size / Price Qty
MAB10557-100
MAB10557-SP
SARS-CoV-2 Spike S2 Subunit Antibody in Data
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SARS-CoV-2 Spike S2 Subunit Antibody Summary

Species Reactivity
SARS-CoV-2
Specificity
Detects SARS-CoV-2 Spike S2 Subunit in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 1034617
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Recombinant SARS-CoV2 Spike S2 Subunit
Met697-Pro1213
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
Recombinant SARS-CoV-2 Spike S2 Subunit Protein (Catalog # 10584-CV)
Simple Western
20 µg/mL
Recombinant SARS-CoV-2 Spike S2 Subunit Protein (Catalog # 10584-CV) and Recombinant SARS-CoV-2 Spike S1/S2 Subunit protein

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot View Larger

Detection of SARS-CoV-2 Spike S2 Subunit and SARS-CoV-2 Spike (Active Trimer) by Western Blot. Western blot shows Recombinant SARS-CoV-2 Spike S2 Subunit Protein (10584-CV) and Recombinant SARS-CoV-2 Spike (Active Trimer) Protein (10549-CV). PVDF membrane was probed with 2 µg/mL of Mouse Anti-SARS-CoV-2 Spike S2 Subunit Monoclonal Antibody (Catalog # MAB10557) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for SARS-CoV-2 Spike S2 Subunit at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Simple Western Detection of SARS-CoV-2 Spike S2 Subunit antibody by Simple Western<sup>TM</sup>. View Larger

Detection of SARS-CoV-2 Spike S2 Subunit by Simple WesternTM. Simple Western lane view shows recombinant SARS-CoV-2 Spike S1 RBD (receptor binding domain) protein (negative sample), recombinant SARS-CoV-2 Spike S1 subunit protein (negative sample), recombinant SARS-CoV-2 Spike S2 subunit protein, recombinant SARS-CoV-2 Spike S1 /S2 Subunit protein, and recombinant SARS-CoV-2 Nucleocapsid protein (negative sample), loaded at 0.2 mg/mL. Specific bands were detected for SARS-CoV-2 Spike S2 Subunit at approximately 91kDa and SARS-CoV-2 Spike S1 /S2 Subunit at approximately 230 kDa (as indicated) using 20 µg/mL of Mouse Anti-SARS-CoV-2 Spike S2 Subunit Monoclonal Antibody (Catalog # MAB10557). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Spike S2 Subunit

SARS-CoV-2, which causes the global pandemic coronavirus disease 2019 (Covid-19), belongs to a family of viruses known as coronaviruses that are commonly comprised of four structural proteins: Spike protein(S), Envelope protein (E), Membrane protein (M), and Nucleocapsid protein (N) (1). SARS-CoV-2 Spike Protein (S Protein) is a glycoprotein that mediates membrane fusion and viral entry. The S protein is homotrimeric, with each ~180-kDa monomer consisting of two subunits, S1 and S2 (2). In SARS-CoV-2, as with most coronaviruses, proteolytic cleavage of the S protein into two distinct peptides, S1 and S2 subunits, is required for activation. The S1 subunit is focused on attachment of the protein to the host receptor while the S2 subunit is involved with cell fusion (3-5).

Long Name
Spike Protein, S2 Subunit
Alternate Names
COVID-19; S2 protein; SARS-CoV-2 Spike; SARS-CoV-2; Spike glycoprotein Subunit 2; Spike protein S2; spike protein; Spike S2 Subunit; structural protein; surface glycoprotein

Product Datasheets

FAQs

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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