SARS-CoV-2 Spike S2 Subunit Antibody

MAB10557 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10557-100
MAB10557-SP

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Detection of SARS-CoV-2 Spike S2 Subunit and SARS-CoV-2 Spike (Active Trimer) by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

SARS-CoV-2 Spike S2 Subunit Antibody Summary

Species Reactivity

SARS-CoV-2

Specificity

Detects SARS-CoV-2 Spike S2 Subunit in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 1034617

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Recombinant SARS-CoV2 Spike S2 Subunit
Met697-Pro1213

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

Recombinant SARS-CoV-2 Spike S2 Subunit Protein (Catalog # 10584-CV)

Simple Western

20 µg/mL

Recombinant SARS-CoV-2 Spike S2 Subunit Protein (Catalog # 10584-CV) and Recombinant SARS-CoV-2 Spike S1/S2 Subunit protein

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot

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Detection of SARS-CoV-2 Spike S2 Subunit and SARS-CoV-2 Spike (Active Trimer) by Western Blot. Western blot shows Recombinant SARS-CoV-2 Spike S2 Subunit Protein (10584-CV) and Recombinant SARS-CoV-2 Spike (Active Trimer) Protein (10549-CV). PVDF membrane was probed with 2 µg/mL of Mouse Anti-SARS-CoV-2 Spike S2 Subunit Monoclonal Antibody (Catalog # MAB10557) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for SARS-CoV-2 Spike S2 Subunit at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Simple Western

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Detection of SARS-CoV-2 Spike S2 Subunit by Simple Western^TM. Simple Western lane view shows recombinant SARS-CoV-2 Spike S1 RBD (receptor binding domain) protein (negative sample), recombinant SARS-CoV-2 Spike S1 subunit protein (negative sample), recombinant SARS-CoV-2 Spike S2 subunit protein, recombinant SARS-CoV-2 Spike S1 /S2 Subunit protein, and recombinant SARS-CoV-2 Nucleocapsid protein (negative sample), loaded at 0.2 mg/mL. Specific bands were detected for SARS-CoV-2 Spike S2 Subunit at approximately 91kDa and SARS-CoV-2 Spike S1 /S2 Subunit at approximately 230 kDa (as indicated) using 20 µg/mL of Mouse Anti-SARS-CoV-2 Spike S2 Subunit Monoclonal Antibody (Catalog # MAB10557). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Spike S2 Subunit

SARS-CoV-2, which causes the global pandemic coronavirus disease 2019 (Covid-19), belongs to a family of viruses known as coronaviruses that are commonly comprised of four structural proteins: Spike protein(S), Envelope protein (E), Membrane protein (M), and Nucleocapsid protein (N) (1). SARS-CoV-2 Spike Protein (S Protein) is a glycoprotein that mediates membrane fusion and viral entry. The S protein is homotrimeric, with each ~180-kDa monomer consisting of two subunits, S1 and S2 (2). In SARS-CoV-2, as with most coronaviruses, proteolytic cleavage of the S protein into two distinct peptides, S1 and S2 subunits, is required for activation. The S1 subunit is focused on attachment of the protein to the host receptor while the S2 subunit is involved with cell fusion (3-5).

Long Name

Spike Protein, S2 Subunit

Alternate Names

COVID-19; S2 protein; SARS-CoV-2 Spike; SARS-CoV-2; Spike glycoprotein Subunit 2; Spike protein S2; spike protein; Spike S2 Subunit; structural protein; surface glycoprotein