TGF-beta 5 Antibody

  
  • Specificity
    Detects TGF‑ beta 5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with the other TGF-beta variants is observed. Neutralizes the biological activity of recombinant amphibian TGF-beta 5, but not TGF-beta 1, porcine TGF-beta 1.2, TGF-beta 2, or TGF-beta 3.
  • Source
    Polyclonal Goat IgG
  • Purification
    Protein A or G purified
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant amphibian TGF-beta 5
    Gly271-Ser382
    Accession # P16176
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Amphibian TGF-beta 5 (Catalog # 245-B5)
  • Neutralization
    Measured by its ability to neutralize TGF‑ beta 5 inhibition of IL‑4-dependent proliferation in the HT‑2 mouse T cell line. Tsang, M. et al. (1995) Cytokine 7:389. The Neutralization Dose (ND50) is typically 0.03-0.08 µg/mL in the presence of 0.1 ng/mL Recombinant Amphibian TGF‑ beta 5 and 7.5 ng/mL Recombinant Mouse IL‑4.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
TGF‑ beta 5 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by TGF‑ beta 5 Antibody. Recombinant Amphibian TGF‑ beta 5 (Catalog # 245-B5) inhibits Recombinant Mouse IL‑4 (Catalog # 404-ML) induced proliferation in the HT‑2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL‑4 (7.5 ng/mL) activity elicited by Recombinant Amphibian TGF‑ beta 5 (0.1 ng/mL) is neutral­ized (green line) by increasing concentrations of Goat Anti-TGF‑ beta 5 Polyclonal Antibody (Catalog # AB-245-NA). The ND50 is typically 0.03‑0.08 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 1 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TGF-beta 5

TGF-beta 5 is a member of the TGF-beta family of growth factors. These proteins are stable, multifunctional factors with a wide variety of effects on the growth and differentiation of virtually all cell types. These actions on growth or differentiation may be stimulatory or inhibitory, depending on the cell type, growth conditions, state of differentiation and on the presence of other growth factors. The full range of in vitro biological activities of TGF-beta 5 has not yet been explored. However, TGF-beta 1, TGF-beta 2, TGF-beta 3, and TGF-beta 5 have been found to be largely interchangeable in an inhibitory bioassay, and it is anticipated that TGF-beta 5 will show a spectrum of activities similar to the other TGF-beta family members. The in vivo role of TGF-beta 5 is also not known, but the presence of this factor in Xenopus embryos, tadpole cells, and adult tissues suggests a role in the mediation of differentiation and development. Similar roles have been suggested for other members of the TGF-beta family. To date, the production of TGF-beta 5 has only been demonstrated in Xenopus.

  • Long Name:
    Transforming Growth Factor beta 5
  • Entrez Gene IDs:
    397778 (Amphibian)
  • Alternate Names:
    TGFbeta 5; TGF-beta 5
Related Research Areas
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

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Chicken Anti-Goat IgG Biotinylated Antibody

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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

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Rabbit Anti-Goat IgG Biotinylated Antibody

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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

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