Viral Japanese Encephalitis virus NS1 Antibody

Catalog # Availability Size / Price Qty
MAB10006-100
MAB10006-SP
    Detection  of Japanese Encephalitis virus NS1 by Western Blot.
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Viral Japanese Encephalitis virus NS1 Antibody Summary

Species Reactivity
Viral
Specificity
Detects Japanese Encephalitis virus NS1 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 995010
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human embryonic kidney cell, HEK293-derived Japanese Encephalitis virus NS1
Thr796-Ala1146
Accession # P32886
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection  of Japanese Encephalitis virus NS1 by Western Blot. View Larger

Detection of Japanese Encephalitis virus NS1 by Western Blot. Western blot shows recombinant Japanese Encephalitis virus NS1 (rvJEV NS1). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Viral Japanese Encephalitis virus NS1 Monoclonal Antibody (Catalog # MAB10006) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Japanese Encephalitis virus NS1 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Japanese Encephalitis virus NS1

Japanese encephalitis virus (JEV) non-structural protein 1 (NS1) is produced by the Japanese encephalitis virus (JEV), a mosquito-borne flavivirus that is the leading cause of virus-induced encephalitis globally (1). Flavivirus NS1 is a multifunctional virulence factor. The glycosylated NS1 exists as a membrane-associated dimer after translocation into the endoplasmic reticulum lumen, where it is essential for viral genome replication (2). The secreted hexamer NS1 is involved in immune evasion and pathogenesis; it is identified as a potential diagnostic marker for early detection of the virus infections (2). Mature viral JEV NS1 contains 352 amino acids and shares high structural similarity to other flavivirus NS1 proteins, such as DENV and ZIKV.

References
  1. Nain, M. et al. (2016) Rev. Med. Virol. 26:129.
  2. Rastogi, M. et al. (2016) Virol. J. 13:131.
Entrez Gene IDs
1489713 (Human)
Alternate Names
Japanese Encephalitis virus NS1; JE virus; JEV; JEVgp1; JEV-NS1

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2B Antibody

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