Viral Japanese Encephalitis virus NS1 Antibody

Catalog # Availability Size / Price Qty
Detection of Japanese Encephalitis virus NS1 by Western Blot.
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Product Details
Citations (1)
Supplemental Products

Viral Japanese Encephalitis virus NS1 Antibody Summary

Species Reactivity
Detects Japanese Encephalitis virus NS1 in direct ELISAs.
Monoclonal Mouse IgG2B Clone # 995010
Protein A or G purified from hybridoma culture supernatant
Human embryonic kidney cell, HEK293-derived Japanese Encephalitis virus NS1
Accession # P32886
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Japanese Encephalitis virus NS1 antibody by Western Blot. View Larger

Detection of Japanese Encephalitis virus NS1 by Western Blot. Western blot shows recombinant Japanese Encephalitis virus NS1 (rvJEV NS1). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Viral Japanese Encephalitis virus NS1 Monoclonal Antibody (Catalog # MAB10006) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Japanese Encephalitis virus NS1 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Japanese Encephalitis virus NS1

Japanese encephalitis virus (JEV) non-structural protein 1 (NS1) is produced by the Japanese encephalitis virus (JEV), a mosquito-borne flavivirus that is the leading cause of virus-induced encephalitis globally (1). Flavivirus NS1 is a multifunctional virulence factor. The glycosylated NS1 exists as a membrane-associated dimer after translocation into the endoplasmic reticulum lumen, where it is essential for viral genome replication (2). The secreted hexamer NS1 is involved in immune evasion and pathogenesis; it is identified as a potential diagnostic marker for early detection of the virus infections (2). Mature viral JEV NS1 contains 352 amino acids and shares high structural similarity to other flavivirus NS1 proteins, such as DENV and ZIKV.

  1. Nain, M. et al. (2016) Rev. Med. Virol. 26:129.
  2. Rastogi, M. et al. (2016) Virol. J. 13:131.
Entrez Gene IDs
1489713 (Viral)
Alternate Names
Japanese Encephalitis virus NS1; Japanese Encephalitis; JE virus; JEV; JEVgp1; JEV-NS1

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Citation for Viral Japanese Encephalitis virus NS1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Comparison of plaque reduction and focus reduction neutralization tests for the measurement of neutralizing antibody titers against japanese encephalitis virus
    Authors: Y Park, AR Kim, YH Hwang, H Yang, JW Lee, MY Kim, HS Kim, GT Chung, JS Yoo, YJ Kim, D Kim, H Won
    Journal of virological methods, 2022-05-10;306(0):114540.
    Species: African Green Monkey
    Sample Types: Whole Cells
    Applications: ICC


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Reconstitution Buffers

Secondary Antibodies

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