Viral Japanese Encephalitis virus NS1 Antibody Summary
Accession # P32886
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Japanese Encephalitis virus NS1 by Western Blot. Western blot shows recombinant Japanese Encephalitis virus NS1 (rvJEV NS1). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Viral Japanese Encephalitis virus NS1 Monoclonal Antibody (Catalog # MAB10006) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Japanese Encephalitis virus NS1 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Japanese Encephalitis virus NS1
Japanese encephalitis virus (JEV) non-structural protein 1 (NS1) is produced by the Japanese encephalitis virus (JEV), a mosquito-borne flavivirus that is the leading cause of virus-induced encephalitis globally (1). Flavivirus NS1 is a multifunctional virulence factor. The glycosylated NS1 exists as a membrane-associated dimer after translocation into the endoplasmic reticulum lumen, where it is essential for viral genome replication (2). The secreted hexamer NS1 is involved in immune evasion and pathogenesis; it is identified as a potential diagnostic marker for early detection of the virus infections (2). Mature viral JEV NS1 contains 352 amino acids and shares high structural similarity to other flavivirus NS1 proteins, such as DENV and ZIKV.
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