Viral Zika Virus (H/PF/2013) Envelope Antibody

Catalog # Availability Size / Price Qty
MAB100091-100
MAB100091-SP
Detection  of Zika Virus (H/PF/2013) Envelope by Western Blot.
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Viral Zika Virus (H/PF/2013) Envelope Antibody Summary

Species Reactivity
Viral
Specificity
Detects Zika Virus (H/PF/2013) Envelope (E3) in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 990844
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant Zika Virus (H/PF/2013) Envelope
Leu590-Ser695
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Viral Zika Virus (H/PF/2013) Envelope Monoclonal Antibody (Catalog # MAB10009).

This product is intended for assay development on various assay platforms requiring antibody pairs.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection  of Zika Virus (H/PF/2013) Envelope by Western Blot. View Larger

Detection of Zika Virus (H/PF/2013) Envelope by Western Blot. Western blot shows recombinant Zika Virus (H/PF/2013) Envelope (rvZIKV-E3). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Viral Zika Virus (H/PF/2013) Envelope Monoclonal Antibody (Catalog # MAB100091) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Zika Virus (H/PF/2013) Envelope at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Zika Virus (H/PF/2013) Envelope

Zika Virus non-structural protein 1 (ZIKV NS1) is an approximately 48 kDa viral glycoprotein (1). Zika Virus is a mosquito-borne flavivirus and has been implicated an association with neonatal microcephaly and neurological disorders such as Guillain-Barré syndrome (2). ZIKV strain H/PF/2013 was isolated from human host.  ZIKV NS1 is a multifunctional virulence factor. The glycosylated NS1 exists as a membrane-associated dimer after translocation into the endoplasmic reticulum lumen, where it is essential for viral genome replication (1, 2). Infected cells secrete NS1 as a lipoprotein, which is involved in immune evasion and pathogenesis via interaction with components of the innate and adaptive immune systems (1, 2). Mature ZIKV NS1 contains 352 amino acids (aa) and has a hexameric conformation consisting of three dimers to form a symmetric barrel shape (1). It has high structural similarity to other flavivirus NS1 proteins, such as DENV and WNV (2, 3). Mature NS1 of ZIKV strain H/PF/2013 shares 98% aa sequence identity with NS1 of ZIKV strain MR 766 that was isolated from monkey host.

References
  1. Song, H. et al. (2016) Nat. Struct. and Mol. Biol. 23:456.
  2. Brown, WC. et al. (2016) Nat. Struct. and Mol. Biol. 23: 865.
  3. Baronti, C. et al. (2014) Genome Announc. 2: e00500
Long Name
Zika Virus Polyprotein
Alternate Names
Zika Virus (H/PF/2013) Envelope; ZIKV_E; ZIKV-E; ZIKV-Env

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Mouse IgG2A Isotype Control

Ctrl

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2A Antibody

Mouse IgG2A Antibody

Reviews for Viral Zika Virus (H/PF/2013) Envelope Antibody

There are currently no reviews for this product. Be the first to review Viral Zika Virus (H/PF/2013) Envelope Antibody and earn rewards!

Have you used Viral Zika Virus (H/PF/2013) Envelope Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review