Canine CD8 alpha Antibody

Catalog # Availability Size / Price Qty
MAB6709
MAB6709-SP
Canine CD8 alpha  Antibody in Data
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Canine CD8 alpha Antibody Summary

Species Reactivity
Canine
Specificity
Detects canine CD8 alpha in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant CD8 from human, mouse, cotton rat, cat, or guinea pig is observed.
Source
Monoclonal Mouse IgG1 Clone # 569126
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant canine CD8 alpha
Ser22-Glu186
Accession # P33706
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry CD8 alpha  in Canine PBMCs. View Larger

CD8 alpha in Canine PBMCs. CD8a was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Canine CD8a alpha Monoclonal Antibody (Catalog # MAB6709) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD8

CD8 is a dimeric complex made up of two Ig superfamily members. The CD8 alpha chain is a 34 kDa type I transmembrane glycoprotein that is disulfide-linked either to itself forming CD8 alpha alpha, or to an unrelated 30‑35 kDa CD8 beta chain, forming CD8 alpha beta. CD8 alpha contains one V-type Ig-like domain in its extracellular region (ECD) that binds to class I MHC molecules. CD8 alpha beta is a TCR coreceptor, while CD8 alpha alpha promotes T cell survival and differentation. Within the ECD, canine CD8 alpha shares 63%, 45%, 43%, 68%, and 54% amino acid identity with human, mouse, rat, feline, and porcine CD8 alpha, respectively.

Entrez Gene IDs
925 (Human); 12525 (Mouse); 24930 (Rat); 102124337 (Cynomolgus Monkey); 493799 (Feline)
Alternate Names
CD_antigen: CD8a; CD8 antigen, alpha polypeptide (p32); CD8; CD8a molecule; CD8A; Leu2 T-lymphocyte antigen; LEU2; MAL; OKT8 T-cell antigen; p32; T cell co-receptor; T8 T-cell antigen; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2

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Cell and Tissue Staining Kits

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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