Canine IL-18/IL-1F4 Antibody

Catalog # Availability Size / Price Qty
Canine IL-18/IL-1F4 Antibody in Immunocytochemistry
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Product Details
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Canine IL-18/IL-1F4 Antibody Summary

Species Reactivity
Detects canine IL‑18/IL‑1F4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant porcine IL-18 is observed, 20% cross-reactivity with recombinant rhesus macaque IL-18 is observed, 15% cross-reactivity with recombinant human IL-18 is observed, and 5% cross-reactivity with recombinant mouse IL-18 and recombinant rat IL-18 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant canine IL‑18/IL‑1F4
Accession # Q9XSR0
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Canine IL‑18/IL‑1F4
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry IL‑18/IL‑1F4 in Canine PBMCs. View Larger

IL‑18/IL‑1F4 in Canine PBMCs. IL-18/IL-1F4 was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) using Goat Anti-Canine IL-18/IL-1F4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2924) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell secretions. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-18/IL-1F4

IL-18, also known as interferon-gamma-inducing factor (IGIF) and IL-18, is an 18 kDa protein belonging to the IL-1 superfamily. It is synthesized as a 24 kDa pro-IL-18 and is processed by caspase 1 to form the active protein. IL-18 is produced by multiple cell types including monocyte/macrophages, dendritic cells, osteoblasts and keratinocytes. Mature IL-18 synergizes with IL-12 to induce differentiation of naïve Th cells into Th1 cells. It also plays multiple roles in other immune cell types including macrophages, NK cells and B cells. Canine and human mature IL-18 share 73% amino acid sequence identity.

Long Name
Interleukin 18
Entrez Gene IDs
3606 (Human); 16173 (Mouse); 29197 (Rat); 397057 (Porcine); 574151 (Primate)
Alternate Names
Iboctadekin; IFN-gamma-inducing factor; IGIF; IGIFIL-1 gamma; IL18; IL-18; IL-18MGC12320; IL-1F4; IL1F4iboctadekin; IL-1g; Interferon gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor); Interleukin-1 gamma; interleukin-18

Product Datasheets


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Cell and Tissue Staining Kits

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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