Canine IL‑18/IL‑1F4 Antibody

Name: Canine IL‑18/IL‑1F4 Antibody
Brand: R&D Systems
SKU: AF2924
Price: 399 USD
Availability: InStock

R&D Systems | Catalog # AF2924

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Canine

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all IL-18/IL-1F4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant canine IL‑18/IL‑1F4
Tyr37-Ser193
Accession # Q9XSR0

Specificity

Detects canine IL‑18/IL‑1F4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant porcine IL-18 is observed, 20% cross-reactivity with recombinant rhesus macaque IL-18 is observed, 15% cross-reactivity with recombinant human IL-18 is observed, and 5% cross-reactivity with recombinant mouse IL-18 and recombinant rat IL-18 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Canine IL‑18/IL‑1F4 Antibody

IL‑18/IL‑1F4 in Canine PBMCs.

IL-18/IL-1F4 was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) using Goat Anti-Canine IL-18/IL-1F4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2924) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell secretions. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Canine IL‑18/IL‑1F4 Antibody

Application

Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed canine peripheral blood mononuclear cells

Western Blot

0.1 µg/mL
Sample: Recombinant Canine IL‑18/IL‑1F4

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IL-18/IL-1F4

IL-18, also known as interferon-gamma-inducing factor (IGIF) and IL-18, is an 18 kDa protein belonging to the IL-1 superfamily. It is synthesized as a 24 kDa pro-IL-18 and is processed by caspase 1 to form the active protein. IL-18 is produced by multiple cell types including monocyte/macrophages, dendritic cells, osteoblasts and keratinocytes. Mature IL-18 synergizes with IL-12 to induce differentiation of naïve Th cells into Th1 cells. It also plays multiple roles in other immune cell types including macrophages, NK cells and B cells. Canine and human mature IL-18 share 73% amino acid sequence identity.