Canine IL-6 Biotinylated Antibody

(1 citations)   
  • Species Reactivity
    Canine
  • Specificity
    Detects canine IL‑6 in ELISAs and Western blots. In sandwich immunoassays, less than 5% cross-reactivity with recombinant feline IL-6 and recombinant porcine IL‑6 is observed and less than 0.2% cross-reactivity with recombinant human IL-6, recombinant mouse IL-6, recombinant rat IL-6, recombinant cotton rat IL-6, and recombinant equine IL-6 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant canine IL‑6
    Thr23-Met207
    Accession # P41323
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Canine IL‑6 (Catalog # 1609-CL)
  • Immunocytochemistry
    5-15 µg/mL
    See below
    • Canine IL-6 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    0.2-0.8 µg/mL 
    Canine IL‑6 Antibody (Catalog # AF1609)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Canine IL‑6 Biotinylated Antibody (Catalog # BAF1609)
  • ELISA Standard
     
    Recombinant Canine IL-6 Protein (Catalog # 1609-CL)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
IL‑6 in Canine PBMCs. IL‑6 was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) treated with Concanavalin A using Goat Anti-Canine IL‑6 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1609) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-6

Interleukin 6 (IL-6) is a pleiotropic alpha -helical cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 activity is central to the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. It is secreted by multiple cell types as a 22-28 kDa phosphorylated and variably glycosylated molecule (1-4). Mature canine IL-6 is 187 amino acids (aa) in length and shares 76%, 59%, 38%, and 40% aa sequence identity with feline, human, mouse, and rat IL-6, respectively (5). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R, triggering IL-6 R association with gp130 and gp130 dimerization (6). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (7). Soluble forms of IL-6 R are generated by both alternate splicing and proteolytic cleavage (3). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R elicit responses from gp130-expressing cells that lack cell surface IL-6 R (3). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous while that of IL-6 R is predominantly restricted to hepatocytes, leukocytes, and lymphocytes (3). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R but not from other cytokines that utilize gp130 as a coreceptor (4, 8).

  • References:
    1. Van Snick, J. (1990) Annu. Rev. Immunol. 8:253.
    2. Hodge, D.R. et al. (2005) Eur. J. Cancer 41:2502.
    3. Jones, S.A. (2005) J. Immunol. 175:3468.
    4. Rose-John, S. et al. (2006) J. Leukoc. Biol. 80:227.
    5. Kukielka, G.L. et al. (1995) Circulation 92:1866.
    6. Murakami, M. et al. (1993) Science 260:1808.
    7. Muller-Newen, G. (2003) Sci. STKE 2003:PE40.
    8. Mitsuyama, K. et al. (2006) Clin. Exp. Immunol. 143:125.
  • Long Name:
    Interleukin 6
  • Entrez Gene IDs:
    3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 100034196 (Equine); 493687 (Feline); 100008733 (Rabbit)
  • Alternate Names:
    B cell stimulatory factor-2; B-cell differentiation factor; BSF-2; BSF2CTL differentiation factor; CDF; HGFHSFIFNB2Hybridoma growth factor; IFN-beta 2; IFN-beta-2; IL6; IL-6; IL-6B-cell stimulatory factor 2; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 1 of 1

  1. Autocrine effects of interleukin-6 mediate acute-phase proinflammatory and tissue-reparative transcriptional responses of canine bladder mucosa.
    Authors: Wood MW, Breitschwerdt EB, Gookin JL
    Infect. Immun., 2011;79(2):708-15.
    Species: Canine
    Sample Type: Whole Tissue
    Application: IHC Frozen
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Biotinylated Control

Ctrl BAF108 17
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Molecular Weight Markers
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Full-Range Molecular Weight Marker, Biotinylated

MW001
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Staining Reagents
Description Application Cat# Citations Images  

Streptavidin-Phycoerythrin

Flow F0040 5
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Streptavidin-Alkaline Phosphatase

ELISA(Det) AR001 1
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NorthernLights Streptavidin NL557

Flow, IHC, ICC NL999 4
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NorthernLights Streptavidin NL493

Flow, IHC, ICC NL997 1
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Avidin-HRP

WB A-115 1
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NorthernLights Streptavidin NL637

Flow, IHC, ICC NL998 1
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Streptavidin-Allophycocyanin

Flow F0050 1
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Avidin-Fluorescein

Flow F0030
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Supplemental Cell Selection Products
Description Application Cat# Citations Images  

MagCellect Streptavidin Ferrofluid

MAG999B 4
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MagCellect Streptavidin Ferrofluid

MAG999 4
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Supplemental ELISA Products
Description Application Cat# Citations Images  

Streptavidin-HRP (set of 5 vials enough for 75 plates total)

ELISA DY998 33
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EvenCoat Streptavidin Coated Plates, 15 Pack

ELISA CP004
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EvenCoat Streptavidin Coated Plates, 5 Pack

ELISA CP003 2
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