Cotton Rat CCL3/MIP-1 alpha Antibody

(1 citations)   
  • Species Reactivity
    Cotton Rat
  • Specificity
    Detects cotton rat CCL3/MIP-1 alpha in direct ELISAs. In direct ELISAs, this antibody shows approximately 50% cross-reactivity with recombinant human CCL3/MIP‑1 alpha and recombinant cotton rat CCL4/MIP-1 beta and no cross-reactivity with recombinant cotton rat CCL5, recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, recombinant mouse CCL1, 2, 3, 4, 5, 6, 7, 8, 9/10/MIP-1 gamma, 11, 12, 17, 19, 20, 21, 22, 24, 25, 27, 28, or recombinant rat CCL20.
  • Source
    Monoclonal Mouse IgG2A Clone # 175024
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant cotton rat CCL3/MIP-1 alpha
    Ala24-Ala92
    Accession # AAL26704
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Cotton Rat CCL3/MIP‑1 alpha (Catalog # 1061-RM)
  • Neutralization
    Measured by its ability to neutralize CCL3/MIP‑1 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR5. The Neutralization Dose (ND50) is typically 0.05-0.3 µg/mL in the presence of 10 ng/mL Recombinant Cotton Rat CCL3/MIP‑1 alpha.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by CCL3/MIP‑1 alpha and Neutralization by Cotton Rat CCL3/MIP‑1 alpha Antibody. Recombinant Cotton Rat CCL3/MIP‑1 alpha (Catalog # 1061-RM) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Cotton Rat CCL3/MIP‑1 alpha (10 ng/mL) is neutralized (green line) by increasing concentrations of Cotton Rat CCL3/MIP‑1 alpha Monoclonal Antibody (Catalog # MAB1061). The ND50 is typically 0.05-0.3 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL3/MIP-1 alpha

MIP-1 alpha is a beta family (CC) chemokine and has been designated CCL3. MIP-1 alpha and CCL4/MIP-1 beta, two closely related but distinct proteins, were originally purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Cotton rat CCL3 cDNA encodes a 92 amino acid (aa) precursor protein with a 23 aa putative signal peptide. Mature cotton rat CCL3 shares approximately 70% amino acid identity with human CCL3. CCL3 is expressed in a wide variety of cells, including lymphocytes, fibroblasts, epithelial cells, and monocytes/macrophages. CCL3 has been shown to play an important role in the recruitment of mononuclear cells. Additionally, CCL3 has been reported to have chemoattractant and adhesive effects on lymphocytes, preferentially promoting the chemotaxis of Th1 cells. CCL3 has also been shown to attract B cells, eosinophils, and dendritic cells. In addition, CCL3 augments cytolytic activity of NK cells (1). CCL3 has been identified as a stem cell inhibitory factor that can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. It has been demonstrated that CCL3 can bind the chemokine receptors CCR1 and CCR5 (2).

  • References:
    1. Robertson, M. (2002) J. Leukoc. Biol. 71:173.
    2. Zlotnik, A. et al. (2000) Immunity 12:121.
  • Entrez Gene IDs:
    6348 (Human); 20302 (Mouse); 25542 (Rat); 448787 (Canine)
  • Alternate Names:
    C-C motif chemokine 3; MIP1-(a); AI323804; CCL3; chemokine (C-C motif) ligand 3; G0S19-1; LD78a; LD78alpha; MIP1 alpha; MIP-1 alpha; Mip1a; MIP-1alpha; MIP1-alpha; Scya3
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Prothymosin alpha lacking the nuclear localization signal as an effective gene therapeutic strategy in collagen-induced arthritis.
    Authors: Shiau AL, Chen SY, Chang MY
    J. Immunol., 2007;178(7):4688-94.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
Isotype Controls
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Mouse IgG2A Isotype Control

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Mouse IgG2A Isotype Control

Ctrl MAB003R
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Secondary Antibodies
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Mouse IgG HRP-conjugated Antibody

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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse IgG Antibody

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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Rat Anti-Mouse IgG2A PE-conjugated Antibody

Flow F0129 1  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Rat Anti-Mouse IgG2A APC-conjugated Antibody

Flow F0130  
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Rat Anti-Mouse IgG2A PerCP-conjugated Antibody

Flow F0131  
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Mouse IgG2A Antibody

Flow MAB0032  
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