Cotton Rat IFN‑ gamma Antibody

Name: Cotton Rat IFN‑ gamma Antibody
Brand: R&D Systems
SKU: MAB565
Price: 529 USD
Availability: InStock

R&D Systems | Catalog # MAB565

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Product Specifications
Applications
Preparation & Storage
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Background
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Research Areas

Key Product Details

Species Reactivity

Cotton Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 133816

View all IFN-gamma Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant cotton rat IFN-gamma
His21-Ile170 (Gly150Arg, Tyr165Asn)
Accession # Q9QXX2

Specificity

Detects cotton rat IFN‑ gamma in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant canine, feline, human, mouse, rat, porcine, or rhesus macaque IFN-gamma is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Applications for Cotton Rat IFN‑ gamma Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Cotton Rat IFN-gamma (Catalog # 565-RG)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IFN-gamma

Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature cotton rat IFN-gamma exists as a noncovalently linked homodimer of 20‑25 kDa variably glycosylated subunits (3). It shares 43%‑50% amino acid sequence identity with bovine, canine, equine, feline, human, mouse, rat, rhesus, and porcine IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, up‑regulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).