Detects cotton rat IL‑1 alpha /IL‑1F1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross‑reactivity with recombinant rat (rr) IL-1 alpha is observed and 20% cross-reactivity with recombinant mouse (rm) IL-1 alpha is observed. In Western blots, no cross-reactivity with recombinant human IL-1 alpha, rmIL-1 alpha, recombinant porcine IL-1 alpha, rrIL-1 beta, and rrIL-18 is observed.
Monoclonal Mouse IgG2A Clone # 140221
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant cotton rat IL‑1 alpha /IL‑1F1 Ser115-Pro269 Accession # AAK94011
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Recombinant Cotton Rat IL‑1 alpha /IL‑1F1 (Catalog # 552-R1)
Measured by its ability to neutralize IL‑1 alpha /IL‑1F1-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J. A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 50 pg/mL Recombinant Cotton Rat IL‑1 alpha /IL‑1F1 and 1.25 µg/mL concanavalin A.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by IL‑1 alpha /IL‑1F1 and Neutralization by Cotton Rat IL‑1 alpha / IL‑1F1 Antibody.
Recombinant Cotton Rat IL‑1 alpha /IL‑1F1 (Catalog # 552-R1) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Cotton Rat IL‑1 alpha /IL‑1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Cotton Rat IL‑1 alpha /IL‑1F1 Monoclonal Antibody (Catalog # MAB552). The ND50 is typically 1-5 µg/mL in the presence of concanavalin A (1.25 µg/mL).
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1 alpha/IL-1F1
IL-1 alpha (Interleukin-1 alpha) is a cytokine that is induced during microbial exposure and promotes the release of multiple inflammatory mediators and acute phase proteins. It also supports hematopoietic stem cell expansion, promotes neutrophil mobilization from the bone marrow, and promotes osteoclast formation. IL-1 alpha signals through a receptor complex composed of IL-1 RI and IL-1 RAcP. It also binds the decoy receptor IL-1 RII which associates with IL-1 RAcP but does not transduce signaling.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.