Cotton Rat IL-1 alpha /IL-1F1 Antibody Summary
Accession # AAK94011
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by IL‑1 alpha /IL‑1F1 and Neutral-ization by Cotton Rat IL‑1 alpha / IL‑1F1 Antibody. Recombinant Cotton Rat IL-1a/IL-1F1 (Catalog # 552-R1) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Cotton Rat IL-1a/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Cotton Rat IL-1a/IL-1F1 Monoclonal Antibody (Catalog # MAB552). The ND50is typically 1-5 µg/mL.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1 alpha/IL-1F1
Interleukin 1 (IL-1) is a name that designates two proteins, IL-1 alpha and IL-1 beta, that are the products of distinct genes, but show approximately 25% amino acid sequence identity and recognize the same cell surface receptors. Although IL-1 production is generally considered to be a consequence of inflammation, evidence suggests that IL-1 is also temporarily up‑regulated during bone formation and the menstrual cycle and can be induced in response to nervous system stimulation. In response to stimuli produced by inflammatory agents, infections, or microbial endotoxins, a dramatic increase in the production of IL-1 by macrophages and various other cells is seen. Cells in particular known to produce IL-1 include osteoblasts, monocytes, macrophages, keratinocytes, Kupffer cells, hepatocytes, thymic and salivary gland epithelium, Schwann cells, fibroblasts and glia (oligodendroglia, astrocytes and microglia).
IL-1 alpha and IL-1 beta are both synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17,000 Daltons. Neither precursor contains a typical hydrophobic signal peptide sequence and most of the precursor form of IL‑1 alpha remains in the cytosol of cells, although there is evidence for a membrane-bound form of the precursor form of IL-1 alpha. The IL-1 alpha precursor reportedly shows full biological activity in the EL-4 assay. Among various species, the amino acid sequence of mature IL-1 alpha is conserved 60% to 70% and human, porcine, rat and cotton rat IL-1 has been found to be biologically active on murine cell lines. Both forms of IL-1 bind to the same receptors, designated as type I and type II. Evidence suggests that only the type I receptor is capable of signal transduction and that the type II receptor may function as a decoy, binding IL-1 and thus preventing the binding of IL-1 to the type I receptor.
- Dower, S.K. and J.E. Sims (1994) Guidebook to Cytokines and their receptors, Nicole, N.A. (ed), Oxford University Press, New York, p. 17.
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