Equine IL-1 beta/IL-1F2 Antibody (855403)

Name: Equine IL-1 beta/IL-1F2 Antibody (855403)
Brand: R&D Systems
SKU: MAB8049
Price: 529 USD
Availability: InStock

R&D Systems | Catalog # MAB8049

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Product Specifications
Scientific Data
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Key Product Details

Species Reactivity

Equine

Applications

Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 855403

View all IL-1 beta/IL-1F2 Primary Antibodies »

Product Specifications

Immunogen

E.coli-derived recombinant equine IL-1 beta
Ala116-Ala268 (Glu179Gly, Met188Thr, Thr194Ile, Ser245Lys and Arg256Gln)
Accession # Q28386

Specificity

Detects equine IL-1 beta in ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Equine IL-1 beta/IL-1F2 Antibody (855403)

Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutral-ization by Equine IL‑1 beta /IL‑1F2 Antibody.

Recombinant Equine IL-1 beta /IL-1F2 induces proliferation in the D10.G4.1 mouse helper T cell line in the presence of concanavalin A (1.25 µg/mL) in a dose-depend-ent manner (orange line), as measured by the Resazurin (Catalog # AR002). Under these conditions, proliferation elicited by IL-1 beta /IL-1F2 is neutralized (green line) by increasing concen-trations of Mouse Anti-Equine IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB8049). The ND₅₀ is typically 2-10 µg/mL.

Applications for Equine IL-1 beta/IL-1F2 Antibody (855403)

Application

Recommended Usage

Neutralization

Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J.A. et al. (1987) in Lymphokines and Interferons, aThe Neutralization Dose (ND₅₀) is typically 2-10 μg/mL in the presence of 100 pg/mL Recombinant Equine IL‑1 beta /IL‑1F2.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 27% amino acid (aa) identity in equine. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The equine IL-1 beta cDNA encodes a 268 aa precursor. A 115 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5-7). An alternatively spliced form of equine IL-1 beta has a deletion which encompasses the Caspase-1 cleavage site and potentially results in a membrane-associated form (8). The 17 kDa mature equine IL-1 beta shares 65%-75% aa sequence identity with canine, cotton rat, feline, human, mouse, porcine, rat, and rhesus IL-1 beta.