ERK2 Antibody (2K9N9)

Name: ERK2 Antibody (2K9N9)
Brand: Novus Biologicals
SKU: NBP3-15826
Price: 389 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-15826

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2K9N9 expressed in HEK293

View all ERK2 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human ERK2ERK1/2 (P27361/P28482). FLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINMKARNYLQSLPSKTKVAWAKLFPKSD/LNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINLKARNYLLSLPHKNKVPWNRLFPNADSKALDLLDKMLTFNPHK

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ERK2 Antibody (2K9N9)

Immunohistochemistry: ERK2 Antibody (2K9N9) [ERK2] -

Immunohistochemistry: ERK2 Antibody (2K9N9) [ERK2] - Immunohistochemistry analysis of paraffin-embedded Mouse intestin tissue using ERK2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: ERK2 Antibody (2K9N9) [ERK2] -

Immunohistochemistry: ERK2 Antibody (2K9N9) [ERK2] - Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using ERK2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: ERK2 Antibody (2K9N9) [ERK2] -

Immunohistochemistry: ERK2 Antibody (2K9N9) [ERK2] - Immunohistochemistry analysis of paraffin-embedded Human colon tissue using ERK2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ERK2 Antibody (7I5L7) [NBP3-15826] -

Analysis of extracts of various cell lines using ERK2 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 1 s.

ERK2 Antibody (7I5L7) [NBP3-15826] -

Analysis of lysates from wild type (WT) and ERK2 knockout (KO) HeLa cells using ERK2 Rabbit mAb at 1:2000 dilution incubated at room temperature for 1.5 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 45 s.

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Applications for ERK2 Antibody (2K9N9)

Application

Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1:1000 - 1:4000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ERK2

Extracellular signal regulated kinase 2 (ERK 2) also known as maturation or mitogen

Long Name

Extracellular Signal-regulated Kinase 2

Alternate Names

ERT1, MAPK1, MAPK2, p41mapk, p42mapk, PRKM1, PRKM2

Gene Symbol

MAPK1

Additional ERK2 Products

Product Documents for ERK2 Antibody (2K9N9)

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Certificate of Analysis

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Product Specific Notices for ERK2 Antibody (2K9N9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.