ERK2 Antibody (4C11) - BSA Free

Name: ERK2 Antibody (4C11) - BSA Free
Brand: Novus Biologicals
SKU: NB110-60506
Price: 389 USD
Availability: InStock

Novus Biologicals | Catalog # NB110-60506

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2A Clone # 4C11

Format

BSA Free

View all ERK2 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human ERK2 expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Theoretical MW

41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ERK2 Antibody (4C11) - BSA Free

Western Blot: ERK2 Antibody (4C11)BSA Free [NB110-60506]

Western Blot: ERK2 Antibody (4C11) [NB110-60506] - Analysis using ERK2 mouse mAb against Hela (1), NIH/3T3 (2), MCF-7 (3), HEK293 (4), Jurkat (5), A549 (6), NTERA-2 (7) and SMMC-7721 (8) cell lysate.

Immunocytochemistry/ Immunofluorescence: ERK2 Antibody (4C11) - BSA Free [NB110-60506]

Immunocytochemistry/Immunofluorescence: ERK2 Antibody (4C11) [NB110-60506] - Analysis of Eca-109 cells using ERK2 mouse mAb (green).

Immunohistochemistry-Paraffin: ERK2 Antibody (4C11) - BSA Free [NB110-60506]

Immunohistochemistry-Paraffin: ERK2 Antibody (4C11) [NB110-60506] - Analysis of human lung carcinoma (left) and breast carcinoma (right) showing cytoplasmic localization using ERK2 mouse mAb with DAB staining.

Applications for ERK2 Antibody (4C11) - BSA Free

Application

Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ERK2

ERK2 (also designated extracellular-signal-related kinase 2 or mitogen-activated protein kinase 1), with 360-amino acid protein (about 40kDa), belongs to the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of ERK2 requires its phosphorylation by upstream kinases. ERK2 is located in the cytoplasm of resting cells and translocates into the nucleus upon extracellular stimuli by active transport of a dimer. ERK2 is essential for placental development and ERK2 in the trophoblast compartment may be indispensable for the vascularization of the labyrinth.

Long Name

Extracellular Signal-regulated Kinase 2

Alternate Names

ERT1, MAPK1, MAPK2, p41mapk, p42mapk, PRKM1, PRKM2

Entrez Gene IDs

5594 (Human)

Gene Symbol

MAPK1

Additional ERK2 Products

Product Documents for ERK2 Antibody (4C11) - BSA Free

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Product Specific Notices for ERK2 Antibody (4C11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.