ERK2 Antibody - BSA Free

Name: ERK2 Antibody - BSA Free
Brand: Novus Biologicals
SKU: NBP3-02974
Price: 429 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-02974

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

View all ERK2 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human ERK2 (NP_002736.3). LNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINLKARNYLLSLPHKNKVPWNRLFPNADSKALDLLDKMLTFNPHK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ERK2 Antibody - BSA Free

Western Blot: ERK2 AntibodyAzide and BSA Free [NBP3-02974]

Western Blot: ERK2 Antibody [NBP3-02974] - Western blot analysis of extracts from normal (control) and ERK2 knockout (KO) HeLa cells, using ERK2 antibody (NBP3-02974) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Immunocytochemistry/ Immunofluorescence: ERK2 Antibody - Azide and BSA Free [NBP3-02974]

Immunocytochemistry/Immunofluorescence: ERK2 Antibody [NBP3-02974] - Immunofluorescence analysis of U-2 OS cells using [KO Validated] ERK2 Rabbit pAb (NBP3-02974) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunohistochemistry-Paraffin: ERK2 Antibody - Azide and BSA Free [NBP3-02974]

Immunohistochemistry-Paraffin: ERK2 Antibody [NBP3-02974] - Immunohistochemistry of paraffin-embedded human liver using [KO Validated] ERK2 Rabbit pAb (NBP3-02974) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Western Blot: ERK2 AntibodyAzide and BSA Free [NBP3-02974]

Western Blot: ERK2 Antibody [NBP3-02974] - Western blot analysis of extracts of various cell lines, using ERK2 antibody (NBP3-02974) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Immunohistochemistry-Paraffin: ERK2 Antibody - Azide and BSA Free [NBP3-02974]

Immunohistochemistry-Paraffin: ERK2 Antibody [NBP3-02974] - Immunohistochemistry of paraffin-embedded rat spleen using [KO Validated] ERK2 Rabbit pAb (NBP3-02974) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Western Blot: ERK2 Antibody - Azide and BSA Free [ERK2] -

Western Blot: ERK2 Antibody - Azide and BSA Free [ERK2] - Western blot analysis of lysates from wild type (WT) and ERK2 knockout (KO) HeLa cells using [KO Validated] ERK2 Rabbit pAb at 1:1000 dilution incubated overnight at 4C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit
Exposure time: 90 s.

Western Blot: ERK2 Antibody - Azide and BSA Free [ERK2] -

Western Blot: ERK2 Antibody - Azide and BSA Free [ERK2] - Western blot analysis of various lysates using [KO Validated] ERK2 Rabbit pAb at 1:1000 dilution incubated overnight at 4C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit
Exposure time: 30 s.

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Applications for ERK2 Antibody - BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ERK2

Extracellular signal regulated kinase 2 (ERK 2) also known as maturation or mitogen

Long Name

Extracellular Signal-regulated Kinase 2

Alternate Names

ERT1, MAPK1, MAPK2, p41mapk, p42mapk, PRKM1, PRKM2

Gene Symbol

MAPK1

Additional ERK2 Products

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Product Specific Notices for ERK2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov