Hematopoietic Lineage Depletion

Lineage Depletion Antibodies & Kits

Lineage depletion antibodies and kits can be used to enrich for uncommitted cell populations.

Lineage Depletion Antibodies (Catalog # MLDP1 to MLDP8)

R&D Systems offers a range of lineage marker antibodies optimized to sufficiently bind 1 x 109 bone marrow derived cells. These antibodies can conveniently be used in conjunction with magnetic particle separation systems (Catalog # MAG997) or flow cytometric cell sorting to deplete lineage-committed cells for the enrichment of mouse uncommitted mesenchymal or hematopoietic stem cells.

Analyte Type Clone # Catalog #
Mouse B220/CD45R Monoclonal Rat IgG2B RA3-6B2 MLDP7
Mouse CD3 Monoclonal Rat IgG2B 17A2 MLDP1
Mouse CD4 Monoclonal Rat IgG2B GK1.5 MLDP2
Mouse CD5 Monoclonal Rat IgG2B 53-7.3 MLDP3
Mouse CD8 alpha Monoclonal Rat IgG2B 52-6.7 MLDP4
Mouse Integrin alpha M/CD Hb Monoclonal Rat IgG2B M1/70 MLDP5
Mouse Gr-1/Ly-6G Monoclonal Rat IgG2B RB6-8C5 MLDP6
Mouse TER-119 Monoclonal Rat IgG2B TER-119 MLDP8

MagCellect™ Mouse Hematopoietic Cell Lineage Depletion Kit** (Catalog # MAGM209)

Designed for the enrichment of hematopoietic non-lineage committed cells (lineage negative) from mouse bone marrow. Lineage committed cells (lineage positive) targeted for depletion include T cells, B cells, NK cells, monocytes/macrophages, granulocytes, and erythrocytes. The resulting cell population is highly enriched for SCF R/c-kit/CD117+ cells to a purity of 40-70% (depending on mouse strain) with less than 5% residual lineage positive cells. This kit is for use with the MagCellect Magnet; sold separately (Catalog # MAG997)

Kit Contents

  • 10X Buffer
  • Streptavidin Ferrofluid
  • Blocking Reagent
  • Anti-CD5
  • Anti-TER-119
  • Anti-CD11b
  • Anti-CD45R/B220
  • Anti-Ly-6G/Gr-1
Hematopoietic Lineage Depletion Enrichment of CD117+ Cells
Hematopoietic Lineage Depletion. Lineage marker reactivity on BALB/c bone marrow (BM) cells processed with the MagCellect Mouse Hematopoietic Cell Lineage Depletion Kit (Catalog # MAGM209). Histograms show reactivity of BM cells labeled with the cocktail of biotinylated antibodies included in the kit both before (green histogram) and after (open his­togram) magnetic depletion. Lineage marker reactivity was detected using Streptavidin-PE. Enrichment of CD117+ Cells. CD117 (c-kit) staining of bone marrow lineage negative cells isolated from BALB/c mice using the MagCellect Mouse Hematopoietic Cell Lineage Depletion Kit (Catalog # MAGM209). Histograms reflect reactivity of all viable cells with anti-CD117-PE (Catalog # FAB1356P; light green) or a matched isotype control antibody (open histogram). Inset shows CD117 staining of cells before depletion.

Mouse Hematopoietic Cell Lineage Depletion Protocol

**This product utilizes and/or contains technology licensed from Veridex, LLC, Raritan, New Jersey 08869, USA, which is covered by one or more claims of United States and International patents and/or pending patent applications.