Human 11 beta-HSD2 Antibody (921215)

R&D Systems | Catalog # MAB8630

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Simple Western

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 921215
View all 11 beta-HSD2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human 11 beta ‑HSD2
Met105-Arg405
Accession # P80365

Specificity

Detects human 11 beta ‑HSD2 in direct ELISA and Western Blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human 11 beta-HSD2 Antibody (921215)

Detection of Human 11 beta ‑HSD2 antibody by Western Blot.

Detection of Human 11 beta ‑HSD2 by Western Blot.

Western blot shows lysates of human kidney tissue, human placenta tissue, and human colon tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human 11 beta -HSD2 Monoclonal Antibody (Catalog # MAB8630) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for 11 beta -HSD2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
11 beta -HSD2 antibody in Human Kidney by Immunohistochemistry (IHC-P).

11 beta ‑HSD2 in Human Kidney.

11 beta -HSD2 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human 11 beta -HSD2 Monoclonal Antibody (Catalog # MAB8630) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human 11 beta ‑HSD2 by Simple WesternTM.

Left: Simple Western lane view shows lysates of human placenta, loaded at 1 mg/ml. A specific band was detected for 11 beta ‑HSD2 at approximately 46 kDa (as indicated) using both 2 µg/ml and 10 µg/ml of Mouse Anti-Human 11 beta ‑HSD2 Monoclonal Antibody (Catalog # MAB8630) followed by HRP-conjugated Goat Anti-Mouse Secondary Antibody (Catalog # 042-205). This experiment was conducted under reducing conditions and using the 12-230kDa separation system. Right: Simple Western electropherogram showing the same Mouse Anti-Human 11 beta ‑HSD2 Monoclonal Antibody (Catalog # MAB8630) tested at 2 µg/ml (blue line) and 10 µg/ml (green line) in the human placenta.

Applications for Human 11 beta-HSD2 Antibody (921215)

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney

Simple Western

2-10 µg/mL
Sample: Human placenta

Western Blot

1 µg/mL
Sample: Human kidney tissue, human placenta tissue, and human colon tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: 11 beta-HSD2

The human enzyme HSD-2/11 beta-HSD2 is encoded by the HSD11B2 gene and codes for a 405 aa protein, with high expression in kidney, colon, pancreas and placenta1. HSD-2 modulates intracellular glucocorticoid levels by catalyzing the conversion of cortisol to the inactive metabolite cortisone2. HSD-2 is abundantly expressed in human placenta and controls fetal glucocorticoid levels to protect the fetus from glucocorticoid exposure. Similarly, HSD-2 expression in the distal nephron protects mineralcorticoid receptors from glucocorticoids and helps regulate blood pressure3. Exposure to major environmental pollutant like Cadmium, inhibits activity of HSD-2 which affects the synthesis of glucocorticoids in placenta and thus contribute to developing preeclamptic conditions.

Long Name

11 beta Hydroxysteroid Dehydrogenase 2

Alternate Names

11 betaHSD2, HSD11B2

Entrez Gene IDs

3291 (Human); 15484 (Mouse); 25117 (Rat)

Gene Symbol

HSD11B2

UniProt

P80365

Additional 11 beta-HSD2 Products

Product Documents for Human 11 beta-HSD2 Antibody (921215)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human 11 beta-HSD2 Antibody (921215)

For research use only

Citations for Human 11 beta-HSD2 Antibody (921215)

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Protocols

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