Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat, Feline, Ovine

Applications

Validated:

Immunohistochemistry, Western Blot, Blockade of Receptor-ligand Interaction

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation, Cell Culture

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Activin RIIB Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Activin RIIB
Ser19-Thr134
Accession # CAA54671

Specificity

Detects human Activin RIIB in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse Activin RIIB is observed, and less than 1% cross-reactivity with recombinant human (rh) Activin RIIA, rhActivin RIA, and rhActivin RIB is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human Activin RIIB Antibody

Activin RIIB antibody in Human Placenta by Immunohistochemistry (IHC-P).

Activin RIIB in Human Placenta.

Activin RIIB was detected in immersion fixed paraffin-embedded sections of human placenta (cross-section of chorionic villi) using 5 µg/mL Goat Anti-Human Activin RIIB Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF339) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Porcine Activin RIIB by Western Blot

Detection of Porcine Activin RIIB by Western Blot

Myostatin signaling in longissimus dorsi muscle of low-birth-weight (LBWT) and normal-birth-weight (NBWT) neonatal pigs. (A): Representative western blots from four pairs of LBWT and NBWT pigs (N, NBWT; L, LBWT). (B–G): Protein abundance of myostain, ALK5 and ActRIIB, and protein abundance phosphorylation of smad2/3. Abundance was normalized to alpha -tubulin and phosphorylation normalized to the corresponding non-phospho-proteins. Results are means ± SE. n = 12. Values with different letters differ significantly (P ≤ 0.05). Image collected and cropped by CiteAb from the following open publication (https://journal.frontiersin.org/article/10.3389/fphys.2017.00482/full), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Activin RIIB Antibody

Application
Recommended Usage

Blockade of Receptor-ligand Interaction

In a functional ELISA, 1-3 µg/mL of this antibody will block 50% of the binding of 30 ng/mL of Recombinant Biotinylated Human/Mouse/Rat Activin A to immobilized Recombinant Human Activin RIIB Fc Chimera (Catalog # 339-RB) coated at 2 µg/mL (100 µL/well). At 30 μg/mL, this antibody will block >90% of the binding.

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human placenta (cross-section of chorionic villi)

Western Blot

0.1 µg/mL
Sample: Recombinant Human Activin RIIB Fc Chimera (Catalog # 339-RB)

Reviewed Applications

Read 2 reviews rated 4.5 using AF339 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Activin RIIB

Activin isoforms and other members of the TGF-beta  superfamily exert their biological effects by binding to heteromeric complexes of a type I and a type II serine‑threonine kinase receptor, both of which are essential for signal transduction. Seven type I and five type II receptors, including the two type I and the two type II activin receptors, designated ActR-I(A), ActR-IB, ActR-II(A) and ActR-IIB, have been cloned from mammals. Through alternative mRNA splicing, multiple ActR-IIB isoforms can also be generated, adding to the complexity of the activin receptor system. Different activin isoforms bind with different high-affinities to the various type II isoforms. Type I activin receptors do not bind directly to activin, but will associate with the type II receptor-activin complex and initiate signal transduction. Besides the activin isoforms, ActR-II will also bind inhibin, BMP-2 and BMP-7 with lower affinities. ActR-I can also bind and form signaling complexes with the BMP‑2/7‑bound BMPR-II. Activin type II receptors are highly conserved. Human, mouse and rat type II activin receptors share greater than 98% amino acid sequence homology. Recombinant soluble activin type II receptors bind activin with high affinity, and are potent activin antagonists.

References

  1. Attisano, L. et al. (1996) Mol. and Cell Biol. 16:1066.
  2. Woodruff, T.K. (1998) Biochem. Pharmacology 55:953.

Long Name

Activin Receptor IIB

Alternate Names

ActivinRIIB, ACVR2B

Entrez Gene IDs

93 (Human); 11481 (Mouse)

Gene Symbol

ACVR2B

UniProt

CAA54671

Additional Activin RIIB Products

Product Documents for Human Activin RIIB Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Activin RIIB Antibody

For research use only

Related Research Areas

Activin Family

Citations for Human Activin RIIB Antibody

Customer Reviews for Human Activin RIIB Antibody (2)

4.5 out of 5
2 Customer Ratings
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  • Human Activin RIIB Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Human recombinant antibody
    Species: Human
    Verified Customer | Posted 04/07/2020
    Human Activin RIIB Antibody AF339
  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: bovine fetal ovary
    Species: Other
    Verified Customer | Posted 04/02/2015
    Localization of Activin RIIB in bovine fetal ovaries
    Human Activin RIIB Antibody AF339

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