Human Activin RIIB Antibody

(4 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Activin RIIB in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse Activin RIIB is observed, and less than 1% cross-reactivity with recombinant human (rh) Activin RIIA, rhActivin RIA, and rhActivin RIB is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Activin RIIB
    Ser19-Thr134
    Accession # CAA54671
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human Activin RIIB Fc Chimera (Catalog # 339-RB)
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Blockade of Receptor-ligand Interaction
    In a functional ELISA, 1-3 µg/mL of this antibody will block 50% of the binding of 30 ng/mL of Recombinant Biotinylated Human/Mouse/Rat Activin A to immobilized Recombinant Human Activin RIIB Fc Chimera (Catalog # 339-RB) coated at 2 µg/mL (100 µL/well). At 30 μg/mL, this antibody will block >90% of the binding.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Activin RIIB in Human Placenta. Activin RIIB was detected in immersion fixed paraffin-embedded sections of human placenta (cross-section of chorionic villi) using 5 µg/mL Goat Anti-Human Activin RIIB Antigen Affinity-purified Poly­clonal Antibody (Catalog # AF339) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Activin RIIB

Activin isoforms and other members of the TGF-beta  superfamily exert their biological effects by binding to heteromeric complexes of a type I and a type II serine‑threonine kinase receptor, both of which are essential for signal transduction. Seven type I and five type II receptors, including the two type I and the two type II activin receptors, designated ActR-I(A), ActR-IB, ActR-II(A) and ActR-IIB, have been cloned from mammals. Through alternative mRNA splicing, multiple ActR-IIB isoforms can also be generated, adding to the complexity of the activin receptor system. Different activin isoforms bind with different high-affinities to the various type II isoforms. Type I activin receptors do not bind directly to activin, but will associate with the type II receptor-activin complex and initiate signal transduction. Besides the activin isoforms, ActR-II will also bind inhibin, BMP-2 and BMP-7 with lower affinities. ActR-I can also bind and form signaling complexes with the BMP‑2/7‑bound BMPR-II. Activin type II receptors are highly conserved. Human, mouse and rat type II activin receptors share greater than 98% amino acid sequence homology. Recombinant soluble activin type II receptors bind activin with high affinity, and are potent activin antagonists.

  • References:
    1. Attisano, L. et al. (1996) Mol. and Cell Biol. 16:1066.
    2. Woodruff, T.K. (1998) Biochem. Pharmacology 55:953.
  • Long Name:
    Activin Receptor IIB
  • Entrez Gene IDs:
    93 (Human)
  • Alternate Names:
    activin A receptor, type IIB; Activin receptor type IIB; activin receptor type-2B; Activin RIIB; ActivinRIIB; ACTRIIB; ActR-IIB; ACVR2B; EC 2.7.11; EC 2.7.11.30; MGC116908
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. Postnatal regulation of germ cells by activin: the establishment of the initial follicle pool.
    Authors: Bristol-Gould SK, Kreeger PK, Selkirk CG, Kilen SM, Cook RW, Kipp JL, Shea LD, Mayo KE, Woodruff TK
    Dev. Biol., 2006;298(1):132-48.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  2. Activin and inhibin receptor gene expression in the ewe pituitary throughout the oestrous cycle.
    Authors: Fafioffe A, Ethier JF, Fontaine J, JeanPierre E, Taragnat C, Dupont J
    J. Endocrinol., 2004;182(1):55-68.
    Species: Ovine
    Sample Type: Tissue Homogenates
    Application: WB
  3. Follicle-restricted compartmentalization of transforming growth factor beta superfamily ligands in the feline ovary.
    Authors: Bristol SK, Woodruff TK
    Biol. Reprod., 2004;70(3):846-59.
    Species: Feline
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  4. Regulation of endocytosis of activin type II receptors by a novel PDZ protein through Ral/Ral-binding protein 1-dependent pathway.
    Authors: Matsuzaki T, Hanai S, Kishi H, Liu Z, Bao Y, Kikuchi A, Tsuchida K, Sugino H
    J. Biol. Chem., 2002;277(21):19008-18.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: IP
Cell and Tissue Staining Kits
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Anti-Goat HRP-DAB Cell & Tissue Staining Kit

CTS008 13
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Normal Goat IgG Control

Ctrl AB-108-C 191  
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Goat IgG HRP-conjugated Antibody

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Goat IgG HRP-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

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Goat IgG (H+L) APC-conjugated Antibody

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Goat IgG Horseradish Peroxidase-conjugated Antibody

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Donkey Anti-Goat IgG Antibody

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Donkey Anti-Goat IgG Biotinylated Antibody

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Goat IgG (H+L) Fluorescein-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

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Chicken Anti-Goat IgG Biotinylated Antibody

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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

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