Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Intracellular Staining by Flow Cytometry, Immunocytochemistry, Immunoprecipitation, CyTOF-ready

Cited:

Western Blot, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 23G9
View all ADAM15 Primary Antibodies »

Product Specifications

Immunogen

COS-7 African green monkey SV40 transformed kidney fibroblast-like cell line-derived recombinant human ADAM15
Asp207-Thr696
Accession # Q13444

Specificity

Detects the ectodomain of recombinant human (rh) ADAM15 in direct ELISAs and Western blots. In direct ELISAs, shows 100% cross‑reactivity with recombinant mouse (rm) ADAM15 but does not cross‑react with rhADAM8, 9, 17, 28, rmADAM9, or 10.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human ADAM15 Ectodomain Antibody

ADAM15 antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC).

ADAM15 in MCF‑7 Human Cell Line.

ADAM15 was detected in immersion fixed MCF-7 human breast cancer cell line using Human ADAM15 Ectodomain Monoclonal Antibody (Catalog # MAB935) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

ADAM15 antibody in Human Prostate Cancer Tissue by Immunohistochemistry (IHC-P).

ADAM15 in Human Prostate Cancer Tissue.

ADAM15 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 25 µg/mL Human ADAM15 Ectodomain Monoclonal Antibody (Catalog # MAB935) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of ADAM15 in PC‑3 cells (Positive) & MOLT‑4 cells (Negative).

ADAM15 was detected in immersion fixed PC‑3 human prostate cancer cells (Positive) & absent in MOLT‑4 human acute lymphoblastic leukemia cells (Negative) using Mouse Anti-Human ADAM15 Ectodomain Monoclonal Antibody (Catalog # MAB935) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to Cytoplasmic. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Human ADAM15 by Flow Cytometry

Detection of Human ADAM15 by Flow Cytometry

miR-147b down-regulates cell surface expression of ADAM15 in endothelial cells.At 48 h after transfection, live HUVECs were labeled with a monoclonal anti-ADAM15 ectodomain antibody and a secondary Cy3 conjugate. Cell surface expression was analyzed by flow cytometry (A–B). The bar graph shows quantitative cell surface ADAM15 expression in different treatment groups, each derived from 3 separate experiments (C). Data represent mean ± SEM. * P<0.05 vs. untreated control. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25333931), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human ADAM15 by Flow Cytometry

Detection of Human ADAM15 by Flow Cytometry

miR-147b down-regulates cell surface expression of ADAM15 in endothelial cells.At 48 h after transfection, live HUVECs were labeled with a monoclonal anti-ADAM15 ectodomain antibody and a secondary Cy3 conjugate. Cell surface expression was analyzed by flow cytometry (A–B). The bar graph shows quantitative cell surface ADAM15 expression in different treatment groups, each derived from 3 separate experiments (C). Data represent mean ± SEM. * P<0.05 vs. untreated control. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25333931), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human ADAM15 Ectodomain Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed MCF-7 human breast cancer cell line, PC‑3 human prostate cancer cells (Positive) & absent in MOLT‑4 human acute lymphoblastic leukemia cells (Negative)

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate cancer tissue

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human ADAM15, see our available Western blot detection antibodies

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: MCF‑7 human breast cancer cell line fixed with paraformaldehyde and permeabilized with saponin

Western Blot

1 µg/mL
Sample: Recombinant Human ADAM15 Western Blot Standard (Catalog # WBC027)
under non-reducing conditions only

Reviewed Applications

Read 2 reviews rated 5 using MAB935 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ADAM15

ADAM15 belongs to a family of transmembrane proteins that contain disintegrin and metalloprotease domains. Members of this family have been implicated in cell adhesion via integrin binding and shedding of cell surface molecules.

Long Name

A Disintegrin and Metalloprotease-like Domain 15

Alternate Names

MDC15, Metargidin

Entrez Gene IDs

8751 (Human); 11490 (Mouse)

Gene Symbol

ADAM15

UniProt

Q13444

Additional ADAM15 Products

Product Documents for Human ADAM15 Ectodomain Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human ADAM15 Ectodomain Antibody

For research use only

Related Research Areas

Metalloproteases and Regulators

Citations for Human ADAM15 Ectodomain Antibody

Customer Reviews for Human ADAM15 Ectodomain Antibody (2)

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2 Customer Ratings
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  • Human ADAM15 Ectodomain Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: THP-1 human acute monocytic leukemia cell line and Cell Lysates
    Species: Human
    Verified Customer | Posted 09/14/2019
    Human ADAM15 Ectodomain Antibody MAB935
  • Human ADAM15 Ectodomain Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Adult lung
    Species: Human
    Verified Customer | Posted 09/30/2018
    Human ADAM15 Ectodomain Antibody MAB935

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Protocols

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