Human ADAM15 Ectodomain Antibody

Catalog # Availability Size / Price Qty
MAB935-SP
MAB935-500
MAB935-100
ADAM15 in MCF‑7 Human Cell Line.
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Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (1)

Human ADAM15 Ectodomain Antibody Summary

Species Reactivity
Human
Specificity
Detects the ectodomain of recombinant human (rh) ADAM15 in direct ELISAs and Western blots. In direct ELISAs, shows 100% cross‑reactivity with recombinant mouse (rm) ADAM15 but does not cross-react with rhADAM8, 9, 17, 28, rmADAM9, or 10.
Source
Monoclonal Mouse IgG1 Clone # 23G9
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
COS-7 African green monkey SV40 transformed kidney fibroblast-like cell line-derived recombinant human ADAM15
Asp207-Thr696
Accession # Q13444
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human ADAM15 Western Blot Standard (Catalog # WBC027)
under non-reducing conditions only
Immunohistochemistry
8-25 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human ADAM15, see our available Western blot detection antibodies
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
MCF‑7 human breast cancer cell line fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunocytochemistry ADAM15 in MCF‑7 Human Cell Line. View Larger

ADAM15 in MCF‑7 Human Cell Line. ADAM15 was detected in immersion fixed MCF‑7 human breast cancer cell line using Human ADAM15 Ectodomain Monoclonal Antibody (Catalog # MAB935) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry ADAM15 in Human Prostate Cancer Tissue. View Larger

ADAM15 in Human Prostate Cancer Tissue. ADAM15 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 25 µg/mL Human ADAM15 Ectodomain Monoclonal Antibody (Catalog # MAB935) overnight at
4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ADAM15

ADAM15 belongs to a family of transmembrane proteins that contain disintegrin and metalloprotease domains. Members of this family have been implicated in cell adhesion via integrin binding and shedding of cell surface molecules.

Long Name
A Disintegrin and Metalloprotease-like Domain 15
Entrez Gene IDs
8751 (Human); 11490 (Mouse)
Alternate Names
ADAM 15; ADAM metallopeptidase domain 15; ADAM15; disintegrin-like, and cysteine-rich protein 15; EC 3.4.24; MDC15; MDC-15; Metargidin

Product Datasheets

Citations for Human ADAM15 Ectodomain Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. MicroRNA-147b regulates vascular endothelial barrier function by targeting ADAM15 expression.
    Authors: Chatterjee V, Beard R, Reynolds J, Haines R, Guo M, Rubin M, Guido J, Wu M, Yuan S
    PLoS ONE, 2014;9(10):e110286.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  2. ADAM15 expression is downregulated in melanoma metastasis compared to primary melanoma.
    Authors: Ungerer C, Doberstein K, Burger C, Hardt K, Boehncke WH, Bohm B, Pfeilschifter J, Dummer R, Mihic-Probst D, Gutwein P
    Biochem. Biophys. Res. Commun., 2010;401(3):363-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  3. Homeostatic effects of the metalloproteinase disintegrin ADAM15 in degenerative cartilage remodeling.
    Authors: Bohm BB, Aigner T, Roy B, Brodie TA, Blobel CP, Burkhardt H
    Arthritis Rheum., 2005;52(4):1100-9.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB

FAQs

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Cell and Tissue Staining Kits

ExactaChIP Chromatin Immunoprecipitation Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human ADAM15 Ectodomain Antibody

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Human ADAM15 Ectodomain Antibody
By Anonymous on 09/30/2018
Application: ICC/IF Sample Tested: Adult lung Species: Human