Human ADAM15 Ectodomain Antibody Summary
Accession # Q13444
under non-reducing conditions only
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
ADAM15 in MCF‑7 Human Cell Line. ADAM15 was detected in immersion fixed MCF‑7 human breast cancer cell line using Human ADAM15 Ectodomain Monoclonal Antibody (Catalog # MAB935) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
ADAM15 in Human Prostate Cancer Tissue.
ADAM15 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 25 µg/mL Human ADAM15 Ectodomain Monoclonal Antibody (Catalog # MAB935) overnight at
4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
ADAM15 belongs to a family of transmembrane proteins that contain disintegrin and metalloprotease domains. Members of this family have been implicated in cell adhesion via integrin binding and shedding of cell surface molecules.
Citations for Human ADAM15 Ectodomain Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
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MicroRNA-147b regulates vascular endothelial barrier function by targeting ADAM15 expression.
Authors: Chatterjee V, Beard R, Reynolds J, Haines R, Guo M, Rubin M, Guido J, Wu M, Yuan S
PLoS ONE, 2014;9(10):e110286.
Sample Types: Whole Cells
ADAM15 expression is downregulated in melanoma metastasis compared to primary melanoma.
Authors: Ungerer C, Doberstein K, Burger C, Hardt K, Boehncke WH, Bohm B, Pfeilschifter J, Dummer R, Mihic-Probst D, Gutwein P
Biochem. Biophys. Res. Commun., 2010;401(3):363-9.
Sample Types: Whole Cells
Homeostatic effects of the metalloproteinase disintegrin ADAM15 in degenerative cartilage remodeling.
Authors: Bohm BB, Aigner T, Roy B, Brodie TA, Blobel CP, Burkhardt H
Arthritis Rheum., 2005;52(4):1100-9.
Sample Types: Cell Lysates
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Cell and Tissue Staining Kits
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