ADAM23 in Human Spleen. ADAM23 was detected in immersion fixed paraffin-embedded sections of human spleen using 15 µg/mL Mouse Anti-Human ADAM23 Monoclonal Antibody (Catalog # MAB4974) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
ADAM23 (a disintegrin and metalloprotease domain 23; also MDC3) is a 100 kDa member of the M12B peptidase family of enzymes. It is expressed on fetal neurons in the hippocampus and cerebellum, and serves as a counter-receptor for alpha v beta 3 integrin. The human ADAM23 proprecursor is a 773 amino acid (aa) type I transmembrane (TM) protein. It contains a 227 aa cleavable proregion (aa 60‑286) and a 506 aa extracellular domain (ECD) (aa 287‑792) that is part of a 70 kDa mature molecule. The ECD contains a nonfunctional metalloprotease domain (aa 299‑494), an integrin-binding disintegrin region (aa 511‑585), and a Cys‑rich domain (aa 589‑611). Two splice variants exist. One shows an in-frame 46 aa substitution for aa 787‑832 that generates a soluble form, while a second shows an in‑frame 31 aa TM substitution for aa 787‑817. Over aa 60‑585, human ADAM23 shares 92% aa sequence identity with mouse ADAM23.
A Disintegrin and Metalloproteinase Domain 23
Entrez Gene IDs:
8745 (Human); 23792 (Mouse); 301460 (Rat)
a disintegrin and metalloproteinase domain 23; ADAM metallopeptidase domain 23; disintegrin and metalloproteinase domain-containing protein 23; MDC3; MDC-3; MDC3ADAM 23; MDC-L; Metalloproteinase-like, disintegrin-like, and cysteine-rich protein 3
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