Human ADAM23 Antibody

Catalog # Availability Size / Price Qty
MAB4974
MAB4974-SP
ADAM23 in Human Spleen.
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Product Details
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Reviews (3)

Human ADAM23 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ADAM23 in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 504425
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human ADAM23
Ser60-His585
Accession # O75077
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry ADAM23 antibody in Human Spleen by Immunohistochemistry (IHC-P). View Larger

ADAM23 in Human Spleen. ADAM23 was detected in immersion fixed paraffin-embedded sections of human spleen using 15 µg/mL Mouse Anti-Human ADAM23 Monoclonal Antibody (Catalog # MAB4974) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ADAM23

ADAM23 (a disintegrin and metalloprotease domain 23; also MDC3) is a 100 kDa member of the M12B peptidase family of enzymes. It is expressed on fetal neurons in the hippocampus and cerebellum, and serves as a counter-receptor for alpha v beta 3 integrin. The human ADAM23 proprecursor is a 773 amino acid (aa) type I transmembrane (TM) protein. It contains a 227 aa cleavable proregion (aa 60‑286) and a 506 aa extracellular domain (ECD) (aa 287‑792) that is part of a 70 kDa mature molecule. The ECD contains a nonfunctional metalloprotease domain (aa 299‑494), an integrin-binding disintegrin region (aa 511‑585), and a Cys‑rich domain (aa 589‑611). Two splice variants exist. One shows an in-frame 46 aa substitution for aa 787‑832 that generates a soluble form, while a second shows an in‑frame 31 aa TM substitution for aa 787‑817. Over aa 60‑585, human ADAM23 shares 92% aa sequence identity with mouse ADAM23.

Long Name
A Disintegrin and Metalloproteinase Domain 23
Entrez Gene IDs
8745 (Human); 23792 (Mouse); 301460 (Rat)
Alternate Names
a disintegrin and metalloproteinase domain 23; ADAM metallopeptidase domain 23; ADAM23; disintegrin and metalloproteinase domain-containing protein 23; MDC3; MDC-3; MDC3ADAM 23; MDC-L; Metalloproteinase-like, disintegrin-like, and cysteine-rich protein 3

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FAQs

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

Reviews for Human ADAM23 Antibody

Average Rating: 3.7 (Based on 3 Reviews)

5 Star
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4 Star
66.67%
3 Star
33.33%
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Human ADAM23 Antibody
By Anonymous on 03/11/2019
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human ADAM23 Antibody
By Anonymous on 11/08/2018
Application: ELISA Sample Tested: Serum and Plasma Species: Human and Mouse

Human ADAM23 Antibody
By Anonymous on 11/02/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human