Human ADAMTS1 Antibody Summary
Accession # Q9VHI8
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human ADAMTS1 by Western Blot.
Western blot shows lysates of human ovarian cancer tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ADAMTS1 Monoclonal Antibody (Catalog # MAB2197) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ADAMTS1 at approximately 60-70 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 2.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
ADAMTS1 (a disintegrin and metalloproteinase with thrombospondin motifs 1), also known as METH1, is the founding member of the family of secreted zinc proteases with a multi-domain structure (1‑3). The protein precursors consist of signal peptide and following domains: pro, catalytic, disintegrin-like, TS type 1 motif, cysteine‑rich, spacer and a variable number of TS type 1 motifs. Based on their substrate specificity, ADAMTS1 and associated family members may be key enzymes in degradation of cartilage leading to inflammation and arthritis (4). It is an active protease cleaving alpha -2-macroglobulin (5), aggrecan (6), and versican (7). Compared to ADAMTS4 (aggrecanase 1) and ADAMTS5 (aggrecanase 2), the aggrecanase activity of ADAMTS1 is lower. However, its activity can be enhanced by the binding of cofactor such as fibulin-1 (8). The aggrecanase activity can be inhibited using 5 mM 1,10 Phenanthroline. ADAMTS1 is essential for normal growth and the structure and function of the kidneys, adrenal glands and female reproductive organs (9). It also plays an important role in atherosclerosis (10). It has been shown to inhibit endothelial cell proliferation by direct binding and sequestration of VEGF165 and to inhibit fibroblast migration at high concentrations by binding to FGF-2 (11, 12). The purified rhADAMTS1 starts at the N-terminus of the catalytic domain and ends in the beginning of the spacer region.
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- Shindo, T., et al. (2000) J. Clin. Invest. 105:1345.
- Wight, T.N. (2005) Arterioscler Thromb. Vasc. Biol. 25:12.
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- Krampert, M. et al. (2005) J. Biol. Chem. 280:23844.
Citations for Human ADAMTS1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Breast cancer cells induce stromal fibroblasts to secrete ADAMTS1 for cancer invasion through an epigenetic change.
Authors: Tyan SW, Hsu CH, Peng KL
PLoS ONE, 2012;7(4):e35128.
Sample Types: Whole Cells
ADAMTS9 is a cell-autonomously acting, anti-angiogenic metalloprotease expressed by microvascular endothelial cells.
Authors: Koo BH, Coe DM, Dixon LJ, Somerville RP, Nelson CM, Wang LW, Young ME, Lindner DJ, Apte SS
Am. J. Pathol., 2010;176(3):1494-504.
Sample Types: Cell Lysates
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