Human ADAMTS1 Antibody

(2 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human ADAMTS1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human ADAMTS-L1.2 is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 268104
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human ADAMTS1
    Phe253-Cys724 (predicted)
    Accession # Q9VHI8
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human ADAMTS1 by Western Blot.
Western blot shows lysates of human ovarian cancer tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ADAMTS1 Monoclonal Antibody (Catalog # MAB2197) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ADAMTS1 at approximately 60-70 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 2.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ADAMTS1

ADAMTS1 (a disintegrin and metalloproteinase with thrombospondin motifs 1), also known as METH1, is the founding member of the family of secreted zinc proteases with a multi-domain structure (1‑3). The protein precursors consist of signal peptide and following domains: pro, catalytic, disintegrin-like, TS type 1 motif, cysteine‑rich, spacer and a variable number of TS type 1 motifs. Based on their substrate specificity, ADAMTS1 and associated family members may be key enzymes in degradation of cartilage leading to inflammation and arthritis (4). It is an active protease cleaving alpha -2-macroglobulin (5), aggrecan (6), and versican (7). Compared to ADAMTS4 (aggrecanase 1) and ADAMTS5 (aggrecanase 2), the aggrecanase activity of ADAMTS1 is lower. However, its activity can be enhanced by the binding of cofactor such as fibulin-1 (8). The aggrecanase activity can be inhibited using 5 mM 1,10 Phenanthroline. ADAMTS1 is essential for normal growth and the structure and function of the kidneys, adrenal glands and female reproductive organs (9). It also plays an important role in atherosclerosis (10). It has been shown to inhibit endothelial cell proliferation by direct binding and sequestration of VEGF165 and to inhibit fibroblast migration at high concentrations by binding to FGF-2 (11, 12). The purified rhADAMTS1 starts at the N-terminus of the catalytic domain and ends in the beginning of the spacer region.

  • References:
    1. Vazquez, F. et al. (1999) J. Biol. Chem. 274:23349.
    2. Kuno, K. et al. (1997) J. Biol. Chem. 272:556.
    3. Porter, S. et al. (2005) Biochem. J. 386:15.
    4. Nagase, H. and M. Kashiwagi (2003) Arthritis Res. Ther. 5:94.
    5. Kuno, K. et al. (1999) J. Biol. Chem. 274:18821.
    6. Kuno, K. et al. (2000) FEBS Lett. 478:241.
    7. Russel, D. L. et al. (2003) J. Biol. Chem. 278:42330.
    8. Lee, N., et al. (2005) J. Biol. Chem. 280:34796.
    9. Shindo, T., et al. (2000) J. Clin. Invest. 105:1345.
    10. Wight, T.N. (2005) Arterioscler Thromb. Vasc. Biol. 25:12.
    11. Luque, A. et al. (2003) J. Biol. Chem. 278:23656.
    12. Krampert, M. et al. (2005) J. Biol. Chem. 280:23844.
  • Long Name:
    A Disintegrin-like and Metalloproteinase Domain with Thrombospondin Motifs 1
  • Entrez Gene IDs:
    9510 (Human)
  • Alternate Names:
    a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 1; ADAM metallopeptidase with thrombospondin type 1 motif, 1; ADAM-TS 1; ADAMTS1; ADAM-TS1; ADAMTS-1; C3-C5; METH1; METH-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Breast cancer cells induce stromal fibroblasts to secrete ADAMTS1 for cancer invasion through an epigenetic change.
    Authors: Tyan SW, Hsu CH, Peng KL
    PLoS ONE, 2012;7(4):e35128.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  2. ADAMTS9 is a cell-autonomously acting, anti-angiogenic metalloprotease expressed by microvascular endothelial cells.
    Authors: Koo BH, Coe DM, Dixon LJ, Somerville RP, Nelson CM, Wang LW, Young ME, Lindner DJ, Apte SS
    Am. J. Pathol., 2010;176(3):1494-504.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
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ExactaChIP Chromatin IP Buffer Panel

ChIP ECP001 2
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Mouse IgG2B Isotype Control

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Rat Anti-Mouse IgG2B APC-conjugated Antibody

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Mouse IgG VisUCyte HRP Polymer Antibody

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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

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Rat Anti-Mouse IgG2B PE-conjugated Antibody

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Mouse IgG2B Antibody

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