Human Adiponectin/Acrp30 Antibody Summary
Accession # Q15848
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Adiponectin/Acrp30 by Western Blot. Western blot shows lysates of human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Adiponectin/Acrp30 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1065) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Adiponectin/Acrp30 at approximately 30-32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Adiponectin/Acrp30 by Simple WesternTM.
Simple Western lane view shows lysates of human placenta tissue, loaded at 0.2 mg/mL. A specific band was detected for Adiponectin/Acrp30 at approximately 41 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Adiponectin/Acrp30 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1065) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Adiponectin, also known as Acrp30, is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It is induced during adipocyte differentiation, and its secretion is stimulated by insulin. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. In this context, Adiponectin is an anti-inflammatory agent, but it exerts pro-inflammatory effects in nonmetabolic disorders such as rheumatoid arthritis and inflammatory bowel disease (1-3). Adiponectin interacts with the receptors AdipoR1 and AdipoR2, calreticulin, and Cadherin-13/T-Cadherin, as well as with several growth factors (4-7). Mature human Adiponectin consists of a 60 amino acid (aa) N-terminal collagenous region and a 137 aa C-terminal C1q-like globular domain which can be cleaved by a leukocyte-derived elastase (8-9). Mature human Adiponectin shares 83% and 85% amino acid (aa) sequence identity with mouse and rat Adiponectin, respectively. Adiponectin associates into trimers that may assemble into medium molecular weight (MMW) hexamers and then into > 300 kDa high molecular weight (HMW) oligomers (10-12). The glycosylation of four hydroxylated lysine residues in the collagenous domain is required for the intracellular formation of HMW complexes (13). The various multimeric forms of Adiponectin exhibit distinct tissue specific and gender specific profiles and activities (12, 14).
- Lara-Castro, C. et al. (2007) Curr. Opin. Lipidol. 18:263.
- Tilg, H. and A.R. Moschen (2006) Nat. Rev. Immunol. 6:772.
- Fantuzzi, G. (2008) J. Allergy Clin. Immunol. 121:326.
- Yamauchi, T. et al. (2007) Nat. Med. 13:332.
- Takemura, Y. et al. (2007) J. Clin. Invest. 117:375.
- Hug, C. et al. (2004) Proc. Natl. Acad. Sci. 101:10308.
- Wang, Y. et al. (2005) J. Biol. Chem. 280:18341.
- Maeda, K. et al. (1996) Biochem. Biophys. Res. Commun. 221:286.
- Waki, H. et al. (2005) Endocrinology 146:790.
- Waki, H. et al. (2003) J. Biol. Chem. 278:40352.
- Tsao, T.S. et al. (2003) J. Biol. Chem. 278:50810.
- Wang, Y. et al. (2008) Biochem. J. 409:623.
- Wang, H. et al. (2006) J. Biol. Chem. 281:16391.
- Pajvani, U.B. et al. (2003) J. Biol. Chem. 278:9073.
Citations for Human Adiponectin/Acrp30 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 8
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Sustained High Levels of Both Total and High Molecular Weight Adiponectin in Plasma during the Convalescent Phase of Haemorrhagic Fever with Renal Syndrome Are Associated with Disease Severity
Authors: K Tang, C Zhang, Y Zhang, Y Zhang, R Zhuang, B Jin, Y Ma
J Immunol Res, 2017;2017(0):6468097.
Sample Types: Plasma
Impact of 3-Amino-1,2,4-Triazole (3-AT)-Derived Increase in Hydrogen Peroxide Levels on Inflammation and Metabolism in Human Differentiated Adipocytes
Authors: FJ Ruiz-Ojeda, C Gomez-Llor, CM Aguilera, A Gil, AI Rupérez
PLoS ONE, 2016;11(3):e0152550.
Sample Types: Cell Lysates
Heavy Metal Ion Regulation of Gene Expression: MECHANISMS BY WHICH LEAD INHIBITS OSTEOBLASTIC BONE-FORMING ACTIVITY THROUGH MODULATION OF THE Wnt/beta-CATENIN SIGNALING PATHWAY.
Authors: Beier E, Sheu T, Dang D, Holz J, Ubayawardena R, Babij P, Puzas J
J Biol Chem, 2015;290(29):18216-26.
Sample Types: Cell Lysates
Urinary adiponectin excretion: a novel marker for vascular damage in type 2 diabetes.
Authors: von Eynatten M, Liu D, Hock C, Oikonomou D, Baumann M, Allolio B, Korosoglou G, Morcos M, Campean V, Amann K, Lutz J, Heemann U, Nawroth PP, Bierhaus A, Humpert PM
Sample Types: Urine
Adiponectin and functional adiponectin receptor 1 are expressed by airway epithelial cells in chronic obstructive pulmonary disease.
Authors: Miller M, Cho JY, Pham A, Ramsdell J, Broide DH
J. Immunol., 2009;182(1):684-91.
Sample Types: Whole Tissue
Applications: IHC Paraffin-embedded
Decreased high-molecular-weight adiponectin in gestational diabetes: implications for the pathophysiology of Type 2 diabetes.
Authors: Retnakaran R, Connelly PW, Maguire G, Sermer M, Zinman B, Hanley AJ
Diabet. Med., 2007;24(3):245-52.
Sample Types: Serum
Improved ELISA for selective measurement of adiponectin multimers and identification of adiponectin in human cerebrospinal fluid.
Authors: Ebinuma H, Miida T, Yamauchi T, Hada Y, Hara K, Kubota N, Kadowaki T
Clin. Chem., 2007;53(8):1541-4.
Sample Types: CSF
Applications: Native SDS-Page
Tumor necrosis factor alpha and adiponectin in bone marrow interstitial fluid from patients with acute myeloid leukemia inhibit normal hematopoiesis.
Authors: Iversen PO, Wiig H
Clin. Cancer Res., 2005;11(19):6793-9.
Sample Types: Whole Cells
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