Human Adiponectin/Acrp30 Antibody AF1065: R&D Systems

Human Adiponectin/Acrp30 Antibody

(8 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Adiponectin/Acrp30 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross‑reactivity with recombinant mouse Adiponectin is observed and less than 5% cross-reactivity with recombinant rat Adiponectin is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Adiponectin/Acrp30
    Glu19-Asn244
    Accession # Q15848
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Adiponectin/Acrp30 by Western Blot. Western blot shows lysates of human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Adiponectin/Acrp30 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1065) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Adiponectin/Acrp30 at approximately 30-32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Adiponectin/Acrp30 by Simple WesternTM. Simple Western lane view shows lysates of human placenta tissue, loaded at 0.2 mg/mL. A specific band was detected for Adiponectin/Acrp30 at approximately 41 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Adiponectin/Acrp30 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1065) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Adiponectin/Acrp30
Adiponectin, also known as Acrp30, is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It is induced during adipocyte differentiation, and its secretion is stimulated by insulin. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. In this context, Adiponectin is an anti-inflammatory agent, but it exerts pro-inflammatory effects in nonmetabolic disorders such as rheumatoid arthritis and inflammatory bowel disease (1 - 3). Adiponectin interacts with the receptors AdipoR1 and AdipoR2, calreticulin, and Cadherin-13/T-Cadherin, as well as with several growth factors (4 - 7). Mature human Adiponectin consists of a 60 amino acid (aa) N-terminal collagenous region and a 137 aa C-terminal C1q-like globular domain which can be cleaved by a leukocyte-derived elastase (8 - 9). Mature human Adiponectin shares 83% and 85% amino acid (aa) sequence identity with mouse and rat Adiponectin, respectively. Adiponectin associates into trimers that may assemble into medium molecular weight (MMW) hexamers and then into > 300 kDa high molecular weight (HMW) oligomers (10 - 12). The glycosylation of four hydroxylated lysine residues in the collagenous domain is required for the intracellular formation of HMW complexes (13). The various multimeric forms of Adiponectin exhibit distinct tissue specific and gender specific profiles and activities (12, 14).
  • References:
    1. Lara-Castro, C. et al. (2007) Curr. Opin. Lipidol. 18:263.
    2. Tilg, H. and A.R. Moschen (2006) Nat. Rev. Immunol. 6:772.
    3. Fantuzzi, G. (2008) J. Allergy Clin. Immunol. 121:326.
    4. Yamauchi, T. et al. (2007) Nat. Med. 13:332.
    5. Takemura, Y. et al. (2007) J. Clin. Invest. 117:375.
    6. Hug, C. et al. (2004) Proc. Natl. Acad. Sci. 101:10308.
    7. Wang, Y. et al. (2005) J. Biol. Chem. 280:18341.
    8. Maeda, K. et al. (1996) Biochem. Biophys. Res. Commun. 221:286.
    9. Waki, H. et al. (2005) Endocrinology 146:790.
    10. Waki, H. et al. (2003) J. Biol. Chem. 278:40352.
    11. Tsao, T.S. et al. (2003) J. Biol. Chem. 278:50810.
    12. Wang, Y. et al. (2008) Biochem. J. 409:623.
    13. Wang, H. et al. (2006) J. Biol. Chem. 281:16391.
    14. Pajvani, U.B. et al. (2003) J. Biol. Chem. 278:9073.
  • Entrez Gene IDs:
    9370 (Human); 11450 (Mouse); 246253 (Rat)
  • Alternate Names:
    ACDC; Acrp30; ACRP30ADPN; adipocyte, C1Q and collagen domain containing; Adiponectin; adiponectin, C1Q and collagen domain containing; AdipoQ; ADIPQTL1; ApM1; apM-1; APM1APM-1; C1q and collagen domain-containing protein; GBP28; GBP28apM1; Gelatin-binding protein
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Species
Applications
Sample Type
  1. Sustained High Levels of Both Total and High Molecular Weight Adiponectin in Plasma during the Convalescent Phase of Haemorrhagic Fever with Renal Syndrome Are Associated with Disease Severity
    Authors: K Tang, C Zhang, Y Zhang, Y Zhang, R Zhuang, B Jin, Y Ma
    J Immunol Res, 2017;2017(0):6468097.
    Species: Human
    Sample Type: Plasma
    Application: WB
  2. Impact of 3-Amino-1,2,4-Triazole (3-AT)-Derived Increase in Hydrogen Peroxide Levels on Inflammation and Metabolism in Human Differentiated Adipocytes
    Authors: FJ Ruiz-Ojeda, C Gomez-Llor, CM Aguilera, A Gil, AI Rupérez
    PLoS ONE, 2016;11(3):e0152550.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. Heavy Metal Ion Regulation of Gene Expression: MECHANISMS BY WHICH LEAD INHIBITS OSTEOBLASTIC BONE-FORMING ACTIVITY THROUGH MODULATION OF THE Wnt/beta-CATENIN SIGNALING PATHWAY.
    Authors: Beier E, Sheu T, Dang D, Holz J, Ubayawardena R, Babij P, Puzas J
    J Biol Chem, 2015;290(29):18216-26.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  4. Adiponectin and functional adiponectin receptor 1 are expressed by airway epithelial cells in chronic obstructive pulmonary disease.
    Authors: Miller M, Cho JY, Pham A, Ramsdell J, Broide DH
    J. Immunol., 2009;182(1):684-91.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  5. Urinary adiponectin excretion: a novel marker for vascular damage in type 2 diabetes.
    Authors: von Eynatten M, Liu D, Hock C, Oikonomou D, Baumann M, Allolio B, Korosoglou G, Morcos M, Campean V, Amann K, Lutz J, Heemann U, Nawroth PP, Bierhaus A, Humpert PM
    Diabetes, 2009;58(9):2093-9.
    Species: Human
    Sample Type: Urine
    Application: WB
  6. Decreased high-molecular-weight adiponectin in gestational diabetes: implications for the pathophysiology of Type 2 diabetes.
    Authors: Retnakaran R, Connelly PW, Maguire G, Sermer M, Zinman B, Hanley AJ
    Diabet. Med., 2007;24(3):245-52.
    Species: Human
    Sample Type: Serum
    Application: WB
  7. Improved ELISA for selective measurement of adiponectin multimers and identification of adiponectin in human cerebrospinal fluid.
    Authors: Ebinuma H, Miida T, Yamauchi T, Hada Y, Hara K, Kubota N, Kadowaki T
    Clin. Chem., 2007;53(8):1541-4.
    Species: Human
    Sample Type: CSF
    Application: Native SDS-Page
  8. Tumor necrosis factor alpha and adiponectin in bone marrow interstitial fluid from patients with acute myeloid leukemia inhibit normal hematopoiesis.
    Authors: Iversen PO, Wiig H
    Clin. Cancer Res., 2005;11(19):6793-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

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