Human ADP-Sugar Pyrophosphatase/NUDT5 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human ADP-Sugar Pyrophosphatase/NUDT5 in direct ELISAs.
  • Source
    Monoclonal Mouse IgG2B Clone # 739044
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human ADP-Sugar Pyrophosphatase/NUDT5
    Glu2-Phe219
    Accession # Q9UKK9
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human ADP‑Sugar Pyrophosphatase/NUDT5 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and human liver tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human ADP-Sugar Pyrophosphatase/NUDT5 Monoclonal Antibody (Catalog # MAB6414) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ADP-Sugar Pyrophosphatase/NUDT5 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ADP-Sugar Pyrophosphatase/NUDT5

ADP-Sugar Pyrophosphatase (NUDT5) is a member of the nudix superfamily of enzymes. Members of this family are pyrophosphohydrolases that act upon substrates with the general structure of a nucleoside (Nu) diphosphate (di) linked to another moiety, X (NDP-X) to yield NMP plus P-X (1). Human NUDT5 is a homodimeric enzyme present in the cytosol of most cell types (2). Glu166 and three magnesium ions are important for stabilizing the transition state during the hydrolysis of ADPR (3). NUDT5 has been suggested to play a role in regulating the intracellular levels of ADPR by NO activation through ADP-ribosylation at cysteine residues of the enzyme in macrophages (4). It also may play defensive role against the mutagenesis induced by oxidized deoxyribonucleosides (5, 6).

  • References:
    1. McLennan, A.G.  (2006) Cell Mol. Life Sci. 63:123.
    2. Zha, M. et al. (2006) J. Mol. Biol. 364:1021.
    3. Zha, M. et al. (2008) J. Mol. Biol. 379:568.
    4. Yu, H.N. et al. (2007) Biochem. Biophys. Res. Comm. 354:764.
    5. Hori, M. et al. (2010) Free Radic. Biol. Med. 48:1197.
    6. Kamiya, H. et al. (2009) DNA Repair (Amst) 8:1250.
  • Entrez Gene IDs:
    11164 (Human)
  • Alternate Names:
    EC 3.6.1.-; EC 3.6.1.13; hYSAH1; nucleoside diphosphate linked moiety X-type motif 5; Nucleoside diphosphate-linked moiety X motif 5; nudix (nucleoside diphosphate linked moiety X)-type motif 5; Nudix motif 5; NUDT5; YSA1; YSA1H; YSA1HADP-sugar pyrophosphatase
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

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Mouse IgG VisUCyte HRP Polymer Antibody

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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

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Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

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Rat Anti-Mouse IgG2B PE-conjugated Antibody

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Mouse IgG2B Antibody

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