Human ADP-Sugar Pyrophosphatase/NUDT5 Antibody (739044)

R&D Systems | Catalog # MAB6414

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 739044
View all ADP-Sugar Pyrophosphatase/NUDT5 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human ADP-Sugar Pyrophosphatase/NUDT5
Glu2-Phe219
Accession # Q9UKK9

Specificity

Detects human ADP-Sugar Pyrophosphatase/NUDT5 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human ADP-Sugar Pyrophosphatase/NUDT5 Antibody (739044)

Detection of Human ADP-Sugar Pyrophosphatase/NUDT5 antibody by Western Blot.

Detection of Human ADP‑Sugar Pyrophosphatase/NUDT5 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and human liver tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human ADP-Sugar Pyrophosphatase/NUDT5 Monoclonal Antibody (Catalog # MAB6414) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ADP-Sugar Pyrophosphatase/NUDT5 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human ADP-Sugar Pyrophosphatase/NUDT5 Antibody (739044)

Application
Recommended Usage

Western Blot

0.2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and human liver tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ADP-Sugar Pyrophosphatase/NUDT5

ADP-Sugar Pyrophosphatase (NUDT5) is a member of the nudix superfamily of enzymes. Members of this family are pyrophosphohydrolases that act upon substrates with the general structure of a nucleoside (Nu) diphosphate (di) linked to another moiety, X (NDP-X) to yield NMP plus P-X (1). Human NUDT5 is a homodimeric enzyme present in the cytosol of most cell types (2). Glu166 and three magnesium ions are important for stabilizing the transition state during the hydrolysis of ADPR (3). NUDT5 has been suggested to play a role in regulating the intracellular levels of ADPR by NO activation through ADP-ribosylation at cysteine residues of the enzyme in macrophages (4). It also may play defensive role against the mutagenesis induced by oxidized deoxyribonucleosides (5, 6).

References

  1. McLennan, A.G.  (2006) Cell Mol. Life Sci. 63:123.
  2. Zha, M. et al. (2006) J. Mol. Biol. 364:1021.
  3. Zha, M. et al. (2008) J. Mol. Biol. 379:568.
  4. Yu, H.N. et al. (2007) Biochem. Biophys. Res. Comm. 354:764.
  5. Hori, M. et al. (2010) Free Radic. Biol. Med. 48:1197.
  6. Kamiya, H. et al. (2009) DNA Repair (Amst) 8:1250.

Alternate Names

Nudix motif 5, YSA1, YSA1H

Entrez Gene IDs

11164 (Human)

Gene Symbol

NUDT5

UniProt

Q9UKK9

Additional ADP-Sugar Pyrophosphatase/NUDT5 Products

Product Documents for Human ADP-Sugar Pyrophosphatase/NUDT5 Antibody (739044)

Certificate of Analysis

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Product Specific Notices for Human ADP-Sugar Pyrophosphatase/NUDT5 Antibody (739044)

For research use only

Related Research Areas

Phosphatases and Regulators

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