Human Aggrecan (ADAMTS5-cleaved) Antibody

Catalog # Availability Size / Price Qty
Detection of Human Aggrecan (ADAMTS5-cleaved) by Western Blot.
1 Image
Product Details
Citations (1)
Supplemental Products

Human Aggrecan (ADAMTS5-cleaved) Antibody Summary

Species Reactivity
Detects human Aggrecan in direct ELISAs. It specifically recognizes the N-terminal fragment of recombinant human Aggrecan released following cleavage by ADAMTS5.
Monoclonal Mouse IgG2B Clone # 725913
Protein A or G purified from hybridoma culture supernatant
KLHG-conjugated human Aggrecan synthetic peptide
Accession # P16112
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Aggrecan (ADAMTS5-cleaved) antibody by Western Blot. View Larger

Detection of Human Aggrecan (ADAMTS5-cleaved) by Western Blot. Western blot shows recombinant human Aggregan N-terminal and C-terminal fragments. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Aggrecan Monoclonal Antibody (Catalog # MAB64892) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for the Aggrecan N-terminal fragment at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Aggrecan

Aggrecan, together with type II collagen, makes up to 90% of the dry weight of healthy cartilage. It hydrates the collagen network and thus provides cartilage with its properties of compressibility and elasticity. The N-terminal region of human Aggrecan (residues 20‑675) consists of two globular domains (G1 and G2), flanking an interglobular domain (IGD). The IGD contains the cleavage sites for matrix metalloproteinases and aggrecanases.

Entrez Gene IDs
176 (Human)
Alternate Names
ACAN; AGC1; AGC1SEDK; aggrecan core protein; Aggrecan; Cartilage-specific proteoglycan core protein; Chondroitin sulfate proteoglycan 1; Chondroitin sulfate proteoglycan core protein 1; CSPCP; CSPG1; CSPG1aggrecan 1; CSPGCP; large aggregating proteoglycan; MSK16; MSK16AGCAN; SEDK

Product Datasheets

You must select a language.


Citation for Human Aggrecan (ADAMTS5-cleaved) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. CircSPI1_005 ameliorates osteoarthritis by sponging miR-370-3p to regulate the expression of MAP3K9
    Authors: JL Zhou, S Deng, HS Fang, H Peng, QJ Hu
    International immunopharmacology, 2022;110(0):109064.
    Species: Mouse
    Sample Types: Cell Lysate
    Applications: Western Blot


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Aggrecan (ADAMTS5-cleaved) Antibody

There are currently no reviews for this product. Be the first to review Human Aggrecan (ADAMTS5-cleaved) Antibody and earn rewards!

Have you used Human Aggrecan (ADAMTS5-cleaved) Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review