Detection of Human Aggrecan (ADAMTS5-cleaved) by Western Blot.
Western blot shows recombinant human Aggregan N-terminal and C-terminal fragments. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Aggrecan Monoclonal Antibody (Catalog # MAB64892) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for the Aggrecan N-terminal fragment at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Aggrecan, together with type II collagen, makes up to 90% of the dry weight of healthy cartilage. It hydrates the collagen network and thus provides cartilage with its properties of compressibility and elasticity. The N-terminal region of human Aggrecan (residues 20‑675) consists of two globular domains (G1 and G2), flanking an interglobular domain (IGD). The IGD contains the cleavage sites for matrix metalloproteinases and aggrecanases.
Have you used Human Aggrecan (ADAMTS5-cleaved) Antibody?
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.