|Detection of Human Akt3 by Western Blot. Western blot shows lysates of DU145 human prostate carcinoma cell line and MDA‑MB‑231 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Human Akt3 Monoclonal Antibody (Catalog # MAB1463) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Akt3 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.|
Akt3 (v-akt murine thymoma viral oncogene homolog 3), also known as protein kinase B gamma (PKB gamma ) and RAC-gamma serine/threonine kinase (RAC-PK-gamma ), is a member of the Akt family that also includes Akt1 (PKB alpha ) and Akt2 (PKB beta ). These highly homologous kinases are central in such diverse cellular processes as glucose uptake, cell cycle progression, and apoptosis. All three Akts contain an amino-terminal pleckstrin homology domain, a central kinase domain, and a carboxyl-terminal regulatory domain.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided