Human Aldo-keto Reductase 1C3/AKR1C3 Antibody

Catalog # Availability Size / Price Qty
MAB7678
MAB7678-SP
Detection of Human Aldo-keto Reductase 1C3/AKR1C3 by Western Blot.
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Product Details
Citations (2)
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Human Aldo-keto Reductase 1C3/AKR1C3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Aldo-keto Reductase 1C3/AKR1C3 in ELISAs and Western Blot.
Source
Monoclonal Mouse IgG1 Clone # 871701
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Aldo-keto Reductase 1C3/AKR1C3
Asp2-Tyr323
Accession # P42330
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
5 µg/mL
See below
Immunohistochemistry
1-25 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Aldo-keto Reductase 1C3/AKR1C3 antibody by Western Blot. View Larger

Detection of Human Aldo-keto Reductase 1C3/AKR1C3 by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Aldo-keto Reductase 1C3/AKR1C3 Monoclonal Antibody (Catalog # MAB7678) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Aldo-keto Reductase 1C3/AKR1C3 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Aldo-keto Reductase 1C3/AKR1C3 antibody in LNCaP Human Cell Line by Immunocytochemistry (ICC). View Larger

Aldo-keto Reductase 1C3/AKR1C3 in LNCaP Human Cell Line. Aldo-keto Reductase 1C3/AKR1C3 was detected in immersion fixed LNCaP human prostate cancer cell line stimulated with 50 ng/mL Recombinant Human/Mouse/Rat ActivinA (Catalog # 338-AC) for 24 hrs using Mouse Anti-Human Aldo-keto Reductase 1C3/AKR1C3 Monoclonal Antibody (Catalog # MAB7678) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western Detection of Human Aldo-keto Reductase 1C3/AKR1C3 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human Aldo-keto Reductase 1C3/AKR1C3 by Simple WesternTM. Simple Western lane view shows lysates of A549 human lung carcinoma cell line and HepG2 human hepatocellular carcinoma cell line, loaded at 0.5 mg/mL. A specific band was detected for Aldo-keto Reductase 1C3/AKR1C3 at approximately 45 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human Aldo-keto Reductase 1C3/AKR1C3 Monoclonal Antibody (Catalog # MAB7678). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunohistochemistry Aldo-keto Reductase 1C3/AKR1C3 antibody in Human Prostate by Immunohistochemistry (IHC-P). View Larger

Aldo-keto Reductase 1C3/AKR1C3 in Human Prostate. Aldo-keto Reductase 1C3/AKR1C3 was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human Aldo-keto Reductase 1C3/AKR1C3 Monoclonal Antibody (Catalog # MAB7678) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Aldo-keto Reductase 1C3/AKR1C3

AKR1C3 (Aldo-Keto Reductase family 1 member C3; also 17-beta HSD 5, prostaglandin F synthase/PGFS and 3-alpha HSD type 2) is a 35-36 kDa member of the four gene 3-alpha HSD family, aldo-keto reductase superfamily of enzymes. It is expressed by multiple cell types, including prostate epithelium, T cells, and hepatocytes. AKR1C3 generates testosterone and progesterone, catalyzes the conversion of aldehydes and ketones into alcohols, and the mediates the reduction of prostaglandin D2 into PGF2. Human AKR1C3 is 323 amino acids (aa) in length. There are three potential isoform variants. One contains an alternative start site at Met120, a second shows a five aa substitution for aa 1-28, and a third possesses a 15 aa substitution for aa 124-323. Full-length human AKR1C3 shares 73% aa sequence identity with mouse AKR1C3.

Long Name
Aldo-keto Reductase Family 1, Member C3
Entrez Gene IDs
8644 (Human); 105349 (Mouse); 171516 (Rat)
Alternate Names
17-beta-hydroxysteroid dehydrogenase type 5; 3-alpha-HSD type 2; 3-alpha-hydroxysteroid dehydrogenase type 2; AKR1C3; Aldo-keto Reductase 1C3; aldo-keto reductase family 1 member C3; aldo-keto reductase family 1, member C3 (3-alpha hydroxysteroid dehydrogenase, type II); AldoketoReductase 1C3; Chlordecone reductase homolog HAKRb; DD3; DD-3; DD317-beta-HSD 5; DDH1; DDX; Dihydrodiol dehydrogenase 3; Dihydrodiol dehydrogenase type I; dihydrodiol dehydrogenase X; EC 1.1.1; EC 1.1.1.112,3-alpha-HSD type II, brain; EC 1.1.1.188; EC 1.1.1.213; EC 1.1.1.63; EC 1.1.1.64; EC 1.3.1.20; HA1753; HAKRB; HAKRBtype IIb 3-alpha hydroxysteroid dehydrogenase; HAKRe; HSD17B5; hydroxysteroid (17-beta) dehydrogenase 5; Indanol dehydrogenase; KIAA0119hluPGFS; PGFS; Prostaglandin F synthase; Testosterone 17-beta-dehydrogenase 5; Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase

Product Datasheets

Citations for Human Aldo-keto Reductase 1C3/AKR1C3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Aldo-Keto Reductase 1C3 Mediates Chemotherapy Resistance in Esophageal Adenocarcinoma via ROS Detoxification
    Authors: C Zhou, Z Wang, J Li, X Wu, N Fan, D Li, F Liu, PS Plum, S Hoppe, AM Hillmer, A Quaas, F Gebauer, SH Chon, CJ Bruns, Y Zhao
    Cancers, 2021;13(10):.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Androgenic potential of human fetal adrenals at the end of the first trimester
    Authors: I Savchuk, ML Morvan, JP Antignac, K Gemzell-Da, B LeBizec, O Soder, K Svechnikov
    Endocr Connect, 2017;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC

FAQs

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Secondary Antibodies

Reviews for Human Aldo-keto Reductase 1C3/AKR1C3 Antibody

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Human Aldo-keto Reductase 1C3/AKR1C3 Antibody
By Anonymous on 11/08/2021
Application: IHC Sample Tested: Prostate cancer Species: Human

Human Aldo-keto Reductase 1C3/AKR1C3 Antibody
By Anonymous on 01/10/2018
Application: WB Sample Tested: HEK293 human embryonic kidney cell line Species: Human