Detection of ADRA1B in PC‑3 Human Cell Line by Flow Cytometry.
PC‑3 human prostate cancer cell line was stained with Mouse Anti-Human ADRA1B Monoclonal Antibody (Catalog # MAB4730, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha-1B Adrenergic R/ADRA1B
ADRA1B, also known as the alpha -1B-adrenergic receptor, is a 90 kDa 7TM catecholamine receptor with selectivity for epinephrine and norepinephrine. Formation of homooligomers and heterooligomers with ADRA1A enables receptor internalization following ligand binding. ADRA1B functions in diverse settings including vasoconstriction and myocardial contractility, neuronal dopaminergic responses, dendritic cell migration and inflammatory responses, and neuroendocrine regulation of fertility. Human ADRA1B shares 95% amino acid sequence identity with mouse and rat ADRA1B.
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