ADRA1B, also known as the alpha -1B-adrenergic receptor, is a 90 kDa 7TM catecholamine receptor with selectivity for epinephrine and norepinephrine. Formation of homooligomers and heterooligomers with ADRA1A enables receptor internalization following ligand binding. ADRA1B functions in diverse settings including vasoconstriction and myocardial contractility, neuronal dopaminergic responses, dendritic cell migration and inflammatory responses, and neuroendocrine regulation of fertility. Human ADRA1B shares 95% amino acid sequence identity with mouse and rat ADRA1B.
Human alpha-1B Adrenergic R/ADRA1B Antibody
R&D Systems | Catalog # MAB4730
Key Product Details
Species Reactivity
Human
Applications
Intracellular Staining by Flow Cytometry, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 471802
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Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human ADRA1B
Met1-Phe520
Accession # P35368
Met1-Phe520
Accession # P35368
Specificity
Detects human ADRA1B. Stains human ADRA1B transfectants but not irrelevant transfectants.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human alpha-1B Adrenergic R/ADRA1B Antibody
Detection of ADRA1B in PC‑3 Human Cell Line by Flow Cytometry.
PC-3 human prostate cancer cell line was stained with Mouse Anti-Human ADRA1B Monoclonal Antibody (Catalog # MAB4730, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Applications for Human alpha-1B Adrenergic R/ADRA1B Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Sample: PC‑3 human prostate cancer cell line fixed with paraformaldehyde and permeabilized with saponin
Sample: PC‑3 human prostate cancer cell line fixed with paraformaldehyde and permeabilized with saponin
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: alpha-1B Adrenergic R/ADRA1B
Long Name
Alpha-1B Adrenergic Receptor
Alternate Names
a-1B AdrenergicR, ADRA1B, alpha1B AdrenergicR, ALPHA1BAR
Gene Symbol
ADRA1B
UniProt
Additional alpha-1B Adrenergic R/ADRA1B Products
Product Documents for Human alpha-1B Adrenergic R/ADRA1B Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human alpha-1B Adrenergic R/ADRA1B Antibody
For research use only
Related Research Areas
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