Human Aminopeptidase PILS/ARTS1 Antibody Summary
Accession # EAW96077
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Aminopeptidase PILS/ARTS1 in A549 Human Cell Line. Aminopeptidase PILS/ARTS1 was detected in immersion fixed A549 human lung carcinoma cell line using Goat Anti-Human Aminopeptidase PILS/ARTS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2334) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Aminopeptidase PILS/ARTS1 in Human Liver. Aminopeptidase PILS/ARTS1 was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human Aminopeptidase PILS/ARTS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2334) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to hepatocyte cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human Aminopeptidase PILS/ARTS1 by Western Blot. Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line and HDLM-2 human Hodgkin's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Aminopeptidase PILS/ARTS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2334) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Aminopeptidase PILS/ARTS1 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Aminopeptidase PILS/ARTS1
The name of Aminopetidase PILS (Puromycin-Insensitive Leucyl-Specific) describes the two basic properties of this zinc metalloprotease in vitro (1). Also known as ALAP (Adipocyte-derived Leucin AiminoPeptidase), type 1 tumor necrosis factor receptor (TNFR) shedding aminopeptidase regulator and ER aminopeptidase ERAP1 or ERAAP, it is encoded by the ARTS1 gene (2‑4). Aminopeptidase PILS has been identified to regulate antigen presentation, promote TNFR1 ectodomain shedding and associate with hypertension (2‑5).
- Schomburg, L. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) pp. 311, Academic Press, San Diego.
- Cui, X. et al. (2002) J. Clin. Invest. 110:515.
- York, I.A. et al. (2002) Nat. Immunol. 3:1177.
- Serwold, T. et al. (2002) Nature 419:480.
- Yamamoto, N. et al. (2002) Hum. Mutat. 19:251.
Citations for Human Aminopeptidase PILS/ARTS1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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The partial dissociation of MHC class I bound peptides exposes their N terminus to trimming by endoplasmic reticulum aminopeptidase 1
Authors: A Papakyriak, E Reeves, M Beton, H Mikolajek, L Douglas, G Cooper, T Elliott, JM Werner, E James
J. Biol. Chem., 2018;0(0):.
Sample Types: Cell Lysates
Applications: Western Blot
Inositol-Requiring Enzyme 1-Mediated Downregulation of MicroRNA (miR)-146a and miR-155 in Primary Dermal Fibroblasts across ThreeTNFRSF1AMutations Results in Hyperresponsiveness to Lipopolysaccharide
Authors: SR Harrison, T Scambler, L Oubussad, C Wong, M Wittmann, MF McDermott, S Savic
Front Immunol, 2018;9(0):173.
Sample Types: Whole Cells
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