Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry, Simple Western, Immunoprecipitation

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Aminopeptidase PILS/ARTS1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Aminopeptidase PILS/ARTS1
Ala37-Met941
Accession # EAW96077

Specificity

Detects human Aminopeptidase PILS/ARTS1 in direct ELISAs and Western blots. In direct ELISAs, approximately 45% cross‑reactivity with recombinant mouse ARTS1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Aminopeptidase PILS/ARTS1 Antibody

Detection of Human Aminopeptidase PILS/ARTS1 antibody by Western Blot.

Detection of Human Aminopeptidase PILS/ARTS1 by Western Blot.

Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line and HDLM-2 human Hodgkin's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Aminopeptidase PILS/ARTS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2334) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Aminopeptidase PILS/ARTS1 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Aminopeptidase PILS/ARTS1 antibody in A549 Human Cell Line by Immunocytochemistry (ICC).

Aminopeptidase PILS/ARTS1 in A549 Human Cell Line.

Aminopeptidase PILS/ARTS1 was detected in immersion fixed A549 human lung carcinoma cell line using Goat Anti-Human Aminopeptidase PILS/ARTS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2334) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Aminopeptidase PILS/ARTS1 antibody in Human Liver by Immunohistochemistry (IHC-P).

Aminopeptidase PILS/ARTS1 in Human Liver.

Aminopeptidase PILS/ARTS1 was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human Aminopeptidase PILS/ARTS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2334) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to hepatocyte cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human Aminopeptidase PILS/ARTS1 by Simple WesternTM.

Simple Western lane view shows lysates of COLO 205 human colorectal adenocarcinoma cell line and HDLM‑2 human Hodgkin’s lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Aminopeptidase PILS/ARTS1 at approximately 115 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Aminopeptidase PILS/ARTS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2334). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.
Detection of Aminopeptidase PILS/ARTS1 by Western Blot

Detection of Aminopeptidase PILS/ARTS1 by Western Blot

ERAP1 and ERAP2 expression quantitative trait loci. (A) Linkage disequilibrium (LD) plot of the selected SNPs in the ERAP1 and ERAP2 genes. The darkness of diamonds shows the strength of LD, darker colour means stronger LD, the number in the diamonds represent r2 between two SNPs. (B) eQTL analysis of rs27524 SNP in LCLs on ERAP1 (left) and on ERAP2 expression (right). Significance was calculated with one-way ANOVA with linear regression. (C) eQTL analysis of the ERAP1 haplotype. Significance was tested using Kruskal-Wallis test with Dunn’s multiple comparison post-test. (D) ERAP1 and ERAP2 protein (p)QTL effect in LCLs by western blot. (E) pQTL analysis of the ERAP1 haplotypes (left) and of ERAP2 SNP rs2549782 (right) in HL cell lines by western blot. The ratio indicates the relative ERAP1 or ERAP2 protein level normalized by the GAPDH protein level. (F) Representative images of immunohistochemical staining of ERAP1 and ERAP2 in HL tumor tissue sections of nodular sclerosis and mixed cellularity subtype. Arrows indicate Hodgkin Reed-Sternberg cells. Statistically significant changes are indicated by ** = p ≤ 0.01, and *** = p ≤ 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33499248), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Aminopeptidase PILS/ARTS1 by Western Blot

Detection of Aminopeptidase PILS/ARTS1 by Western Blot

ERAP1 and ERAP2 expression quantitative trait loci. (A) Linkage disequilibrium (LD) plot of the selected SNPs in the ERAP1 and ERAP2 genes. The darkness of diamonds shows the strength of LD, darker colour means stronger LD, the number in the diamonds represent r2 between two SNPs. (B) eQTL analysis of rs27524 SNP in LCLs on ERAP1 (left) and on ERAP2 expression (right). Significance was calculated with one-way ANOVA with linear regression. (C) eQTL analysis of the ERAP1 haplotype. Significance was tested using Kruskal-Wallis test with Dunn’s multiple comparison post-test. (D) ERAP1 and ERAP2 protein (p)QTL effect in LCLs by western blot. (E) pQTL analysis of the ERAP1 haplotypes (left) and of ERAP2 SNP rs2549782 (right) in HL cell lines by western blot. The ratio indicates the relative ERAP1 or ERAP2 protein level normalized by the GAPDH protein level. (F) Representative images of immunohistochemical staining of ERAP1 and ERAP2 in HL tumor tissue sections of nodular sclerosis and mixed cellularity subtype. Arrows indicate Hodgkin Reed-Sternberg cells. Statistically significant changes are indicated by ** = p ≤ 0.01, and *** = p ≤ 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33499248), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Aminopeptidase PILS/ARTS1 Antibody

Application
Recommended Usage

Immunocytochemistry

1-15 µg/mL
Sample: Immersion fixed A549 human lung carcinoma cell line

Immunohistochemistry

1-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Aminopeptidase PILS/ARTS1 (Catalog # 2334-ZN), see our available Western blot detection antibodies

Simple Western

10 µg/mL
Sample: COLO 205 human colorectal adenocarcinoma cells and HDLM‑2 human Hodgkin's lymphoma cells

Western Blot

1 µg/mL
Sample: COLO 205 human colorectal adenocarcinoma cell line and HDLM‑2 human Hodgkin's lymphoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Aminopeptidase PILS/ARTS1

The name of Aminopetidase PILS (Puromycin-Insensitive Leucyl-Specific) describes the two basic properties of this zinc metalloprotease in vitro (1). Also known as ALAP (Adipocyte-derived Leucin AiminoPeptidase), type 1 tumor necrosis factor receptor (TNFR) shedding aminopeptidase regulator and ER aminopeptidase ERAP1 or ERAAP, it is encoded by the ARTS1 gene (2‑4). Aminopeptidase PILS has been identified to regulate antigen presentation, promote TNFR1 ectodomain shedding and associate with hypertension (2‑5).

References

  1. Schomburg, L. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) pp. 311, Academic Press, San Diego.
  2. Cui, X. et al. (2002) J. Clin. Invest. 110:515.
  3. York, I.A. et al. (2002) Nat. Immunol. 3:1177.
  4. Serwold, T. et al. (2002) Nature 419:480.
  5. Yamamoto, N. et al. (2002) Hum. Mutat. 19:251.

Long Name

Aminopeptidase Puromycin-insensitive Leucyl-specific

Alternate Names

A-LAP, ARTS1, ERAAP1, ERAP1, PILS-AP

Entrez Gene IDs

51752 (Human); 80898 (Mouse); 102146570 (Cynomolgus Monkey)

Gene Symbol

ERAP1

UniProt

EAW96077

Additional Aminopeptidase PILS/ARTS1 Products

Product Documents for Human Aminopeptidase PILS/ARTS1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Aminopeptidase PILS/ARTS1 Antibody

For research use only

Related Research Areas

Metalloproteases and Regulators

Citations for Human Aminopeptidase PILS/ARTS1 Antibody

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Protocols

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