Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 370207
View all Angiopoietin-like Protein 3/ANGPTL3 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Angiopoietin-like 3
Ser17-Glu460
Accession # Q9Y5C1

Specificity

Detects human ANGPTL3 in direct ELISAs and Western blots. In direct ELISAs, this antibody shows approximately 40% cross‑reactivity with recombinant mouse (rm) ANGPTL3 and no cross-reactivity with rhAng-1, rhAng-2, or rhAng-4.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human Angiopoietin-like 3 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human Angiopoietin-like 3 (Catalog # 3829-AN)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Angiopoietin-like Protein 3/ANGPTL3

Angiopoietin-like 3 (ANGPTL3) is a secreted glycoprotein that belongs to the angiopoietin family. It is expressed in the liver and circulates as 70 kDa, 50 kDa, and 32 kDa species. These include full length ANGPTL3 as well as the proteolytically separated N- and C-terminal fragments which contain a coiled coil domain and fibrinogen-like domain, respectively. ANGPTL3 promotes an increase in circulating triglyceride levels by directly inhibiting lipoprotein lipase. Mature human ANGPTL3 shares 77% amino acid sequence identity with mouse ANGPTL3.

Alternate Names

AGNPT5, Ang-5, Angiopoietin-5, ANGPTL3

Entrez Gene IDs

27329 (Human); 30924 (Mouse); 102136264 (Cynomolgus Monkey)

Gene Symbol

ANGPTL3

UniProt

Q9Y5C1 (Human)

Additional Angiopoietin-like Protein 3/ANGPTL3 Products

Product Documents for Human Angiopoietin-like 3 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Angiopoietin-like 3 Antibody

For research use only

Citations for Human Angiopoietin-like 3 Antibody

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