Key Product Details

Species Reactivity

Human

Applications

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 856606
View all Angiopoietin-like Protein 6/ANGPTL6 Primary Antibodies »

Product Specifications

Immunogen

E. coli-expressed recombinant Human Angiopoietin-like Protein 6/ANGPTL6
Arg21-Leu470
Accession # Q8ROZ6

Specificity

Detects human Angiopoietin-like protein 6/ANGPTL6 in direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Angiopoietin‑like Protein 6/ANGPTL6 Antibody

Human Angiopoietin‑like Protein 6/ANGPTL6 ELISA Standard Curve.

Recombinant Human Angiopoietin‑like Protein 6/ANGPTL6 protein was serially diluted 2-fold and captured by Mouse Anti-Human Angiopoietin‑like Protein 6/ANGPTL6 Monoclonal Antibody (Catalog # MAB11306) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Angiopoietin‑like Protein 6/ANGPTL6 Monoclonal Antibody (MAB113061) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Human Angiopoietin‑like Protein 6/ANGPTL6 Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody when paired with Human Angiopoietin-like Protein 6/ANGPTL6 Monoclonal Antibody (Catalog # MAB113061). 

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Angiopoietin-like 6 DuoSet ELISA Kit (Catalog # DY7846-05) for convenient development of a sandwich ELISA.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Angiopoietin-like Protein 6/ANGPTL6

Angiopoietin-like 6 (ANGPTL6), also known as angiopoietin-related growth factor (AGF), is a secreted 50 kDa protein that contains a coiled-coil domain (aa 51-77,
126-164) and a fibrinogen-like domain (aa 238-456). A conserved Integrin-binding RGD motif is located within the fibrinogen-like domain. This enables ANGPTL6 to promote skin wound healing by mediating the adhesion and migration of keratinocytes, fibroblasts, and endothelial cells. ANGPTL6 also promotes the chemotaxis of vascular endothelial cells resulting in increased vascular permeability and angiogenesis. ANGPTL6 is also secreted by hepatocytes. It inhibits gluconeogenesis in these cells and promotes insulin sensitivity and energy expenditure in mice fed high fat diets. Serum levels of ANGPTL6 are elevated in metabolic syndrome, diabetes, and preeclampsia but are decreased in chronic renal failure. ANGPTL6 is additionally produced by several hematopoietic cell types including megakaryocytes, platelets, mast cells, and uterine NK cells. Mature mouse ANGPTL6 shares 75% and 95% sequence identity with human and rat ANGPTL6.

Alternate Names

AGF, Angiopoietin like Protein 6, ANGPTL6, ARP5

Entrez Gene IDs

83854 (Human); 70726 (Mouse); 298698 (Rat)

Gene Symbol

ANGPTL6

UniProt

Q8ROZ6

Additional Angiopoietin-like Protein 6/ANGPTL6 Products

Product Documents for Human Angiopoietin‑like Protein 6/ANGPTL6 Antibody

Certificate of Analysis

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Product Specific Notices for Human Angiopoietin‑like Protein 6/ANGPTL6 Antibody

For research use only

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