Human Angiopoietin-like Protein 6/ANGPTL6 Antibody Summary
Accession # Q8ROZ6
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Angiopoietin-like 6 DuoSet ELISA Kit (Catalog # DY7846-05) for convenient development of a sandwich ELISA.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Human Angiopoietin‑like Protein 6/ANGPTL6 ELISA Standard Curve. Recombinant Human Angiopoietin‑like Protein 6/ANGPTL6 protein was serially diluted 2-fold and captured by Mouse Anti-Human Angiopoietin‑like Protein 6/ANGPTL6 Monoclonal Antibody (MAB11306) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Angiopoietin‑like Protein 6/ANGPTL6 Monoclonal Antibody (Catalog # MAB113061) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Angiopoietin-like Protein 6/ANGPTL6
Angiopoietin-like 6 (ANGPTL6), also
known as angiopoietin-related growth factor (AGF), is a secreted 50 kDa protein
that contains a coiled-coil domain (aa 51-77,
126-164) and a fibrinogen-like domain (aa 238-456). A conserved Integrin-binding RGD motif is located within the fibrinogen-like domain. This enables ANGPTL6 to promote skin wound healing by mediating the adhesion and migration of keratinocytes, fibroblasts, and endothelial cells. ANGPTL6 also promotes the chemotaxis of vascular endothelial cells resulting in increased vascular permeability and angiogenesis. ANGPTL6 is also secreted by hepatocytes. It inhibits gluconeogenesis in these cells and promotes insulin sensitivity and energy expenditure in mice fed high fat diets. Serum levels of ANGPTL6 are elevated in metabolic syndrome, diabetes, and preeclampsia but are decreased in chronic renal failure. ANGPTL6 is additionally produced by several hematopoietic cell types including megakaryocytes, platelets, mast cells, and uterine NK cells. Mature mouse ANGPTL6 shares 75% and 95% sequence identity with human and rat ANGPTL6.
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