Detects human AP-2 gamma in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human (rh) AP-2 alpha, beta, epsilon and less than 1% cross-reactivity with rhAP-2δ is observed.
Polyclonal Goat IgG
E. coli-derived recombinant human AP-2 gamma Leu128-Val223 Accession # Q92754
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
AP‑2 gamma in Human Breast Cancer Tissue.
AP‑2 gamma was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 10 µg/mL Goat Anti-Human AP‑2 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5059) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human AP‑2 gamma by Western Blot.
Western blot shows lysates of A431 human epithelial carcinoma cell line and human thymus tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human AP‑2 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5059) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for AP‑2 gamma at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: AP-2 gamma
AP-2 gamma (Activation protein 2 gamma; also ERF-1) is a 50-55 kDa member of the AP-2 transcription factor family. It is essential for kidney development and placentation of the embryo. In the nucleus, it forms homodimers and heterodimers with other AP-2 family members. Elevated AP-2 family members are highly suggestive of neoplasia. Human AP-2 gamma is 450 amino acids in length. It contains a repressor SUMOylation site as Lys10, a Gln/Pro-rich transactivation domain (aa 30‑119) and a helix-span-helix dimerization region (aa 293-424). One potential splice form exists that shows a four aa substitution for the N-terminal 16 aa, followed by a premature truncation after Gly130. Over aa 128-223, human AP-2 gamma shares 91% and 82% aa sequence identity with porcine and mouse AP-2 gamma, respectively.
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