Human AP-2 gamma Antibody

Catalog # Availability Size / Price Qty
AF5059
AF5059-SP
AP‑2 gamma  in Human Breast Cancer Tissue.
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Product Details
Citations (3)
FAQs
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Human AP-2 gamma Antibody Summary

Species Reactivity
Human
Specificity
Detects human AP-2 gamma in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) AP-2 alpha is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human AP-2 gamma
Leu128-Val223
Accession # Q92754
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry AP‑2? antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

AP‑2 gamma in Human Breast Cancer Tissue. AP-2 gamma was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 10 µg/mL Goat Anti-Human AP-2 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5059) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western Blot Detection of Human AP‑2? antibody by Western Blot. View Larger

Detection of Human AP‑2 gamma by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line and human thymus tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human AP-2 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5059) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for AP-2 gamma at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AP-2 gamma

AP-2 gamma (Activation protein 2 gamma; also ERF-1) is a 50-55 kDa member of the AP-2 transcription factor family. It is essential for kidney development and placentation of the embryo. In the nucleus, it forms homodimers and heterodimers with other AP-2 family members. Elevated AP-2 family members are highly suggestive of neoplasia. Human AP-2 gamma is 450 amino acids in length. It contains a repressor SUMOylation site as Lys10, a Gln/Pro-rich transactivation domain (aa 30‑119) and a helix-span-helix dimerization region (aa 293-424). One potential splice form exists that shows a four aa substitution for the N-terminal 16 aa, followed by a premature truncation after Gly130. Over aa 128-223, human AP-2 gamma shares 91% and 82% aa sequence identity with porcine and mouse AP-2 gamma, respectively.

Long Name
Activating Enhancer-binding Protein gamma
Entrez Gene IDs
7022 (Human); 21420 (Mouse); 362280 (Rat)
Alternate Names
Activating enhancer-binding protein 2 gamma; AP2 gamma; AP-2 gamma; AP2-GAMMA; ERF1; estrogen receptor factor 1; hAP-2g; TFAP2C; TFAP2G; transcription factor AP-2 gamma (activating enhancer binding protein 2 gamma); transcription factor AP-2 gamma (activating enhancer-binding protein 2 gamma); transcription factor AP-2 gamma; Transcription factor ERF-1

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Citations for Human AP-2 gamma Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Stem cell-derived synthetic embryos self-assemble by exploiting cadherin codes and cortical tension
    Authors: M Bao, J Cornwall-S, E Sanchez-Va, DY Chen, J De Jonghe, S Shadkhoo, F Hollfelder, M Thomson, DM Glover, M Zernicka-G
    Nature Cell Biology, 2022;24(9):1341-1349.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Trophoblast organoids as a model for maternal-fetal interactions during human placentation
    Authors: MY Turco, L Gardner, RG Kay, RS Hamilton, M Prater, MS Hollinshea, A McWhinnie, L Esposito, R Fernando, H Skelton, F Reimann, FM Gribble, A Sharkey, SGE Marsh, S O'Rahilly, M Hemberger, GJ Burton, A Moffett
    Nature, 2018;0(0):.
    Species: Human
    Sample Types: Trophoblast Organoids
    Applications: IHC
  3. Elf5-centered transcription factor hub controls trophoblast stem cell self-renewal and differentiation through stoichiometry-sensitive shifts in target gene networks.
    Authors: Latos P, Sienerth A, Murray A, Senner C, Muto M, Ikawa M, Oxley D, Burge S, Cox B, Hemberger M
    Genes Dev, 2015;29(23):2435-48.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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